{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T22:35:14Z","timestamp":1785278114097,"version":"3.55.0"},"reference-count":98,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2019,4,22]],"date-time":"2019-04-22T00:00:00Z","timestamp":1555891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["141919\/2016-7"],"award-info":[{"award-number":["141919\/2016-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["14\/13783-6"],"award-info":[{"award-number":["14\/13783-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["12\/13961-6"],"award-info":[{"award-number":["12\/13961-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88881.187095\/2018-01"],"award-info":[{"award-number":["88881.187095\/2018-01"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cancer cachexia is a multifactorial syndrome that leads to significant weight loss. Cachexia affects 50%\u201380% of cancer patients, depending on the tumor type, and is associated with 20%\u201340% of cancer patient deaths. Besides the efforts to identify molecular mechanisms of skeletal muscle atrophy\u2014a key feature in cancer cachexia\u2014no effective therapy for the syndrome is currently available. MicroRNAs are regulators of gene expression, with therapeutic potential in several muscle wasting disorders. We performed a meta-analysis of previously published gene expression data to reveal new potential microRNA\u2013mRNA networks associated with muscle atrophy in cancer cachexia. We retrieved 52 differentially expressed genes in nine studies of muscle tissue from patients and rodent models of cancer cachexia. Next, we predicted microRNAs targeting these differentially expressed genes. We also include global microRNA expression data surveyed in atrophying skeletal muscles from previous studies as background information. We identified deregulated genes involved in the regulation of apoptosis, muscle hypertrophy, catabolism, and acute phase response. We further predicted new microRNA\u2013mRNA interactions, such as miR-27a\/Foxo1, miR-27a\/Mef2c, miR-27b\/Cxcl12, miR-27b\/Mef2c, miR-140\/Cxcl12, miR-199a\/Cav1, and miR-199a\/Junb, which may contribute to muscle wasting in cancer cachexia. Finally, we found drugs targeting MSTN, CXCL12, and CAMK2B, which may be considered for the development of novel therapeutic strategies for cancer cachexia. Our study has broadened the knowledge of microRNA-regulated networks that are likely associated with muscle atrophy in cancer cachexia, pointing to their involvement as potential targets for novel therapeutic strategies.<\/jats:p>","DOI":"10.3390\/ijms20081962","type":"journal-article","created":{"date-parts":[[2019,4,22]],"date-time":"2019-04-22T11:02:53Z","timestamp":1555930973000},"page":"1962","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["The Pathway to Cancer Cachexia: MicroRNA-Regulated Networks in Muscle Wasting Based on Integrative Meta-Analysis"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0649-8279","authenticated-orcid":false,"given":"Paula Paccielli","family":"Freire","sequence":"first","affiliation":[{"name":"Department of Morphology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-619, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geysson Javier","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-619, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4803-0933","authenticated-orcid":false,"given":"Sarah Santiloni","family":"Cury","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-619, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diogo","family":"de Moraes","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-619, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakeline Santos","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-619, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grasieli","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-619, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maeli","family":"Dal-Pai-Silva","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-619, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3775-3797","authenticated-orcid":false,"given":"Patr\u00edcia Pintor","family":"dos Reis","sequence":"additional","affiliation":[{"name":"Department of Surgery and Orthopedics, Faculty of Medicine, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-687, Brazil"},{"name":"Experimental Research Unity, Faculty of Medicine, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-687, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4901-7714","authenticated-orcid":false,"given":"Robson Francisco","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biosciences, S\u00e3o Paulo State University (UNESP), Botucatu, S\u00e3o Paulo 18.618-619, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1038\/nrc3829","article-title":"Cancer cachexia: Understanding the molecular basis","volume":"14","author":"Busquets","year":"2014","journal-title":"Nat. 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