{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:46:12Z","timestamp":1767858372618,"version":"3.49.0"},"reference-count":37,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T00:00:00Z","timestamp":1557187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018M3D3A1A01055732"],"award-info":[{"award-number":["2018M3D3A1A01055732"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018M3D3A1A01056181"],"award-info":[{"award-number":["2018M3D3A1A01056181"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1C1B6004086"],"award-info":[{"award-number":["2018R1C1B6004086"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korea Research Institute of Bioscience and Biotechnology Research Initiative Program","award":["KGM5401911"],"award-info":[{"award-number":["KGM5401911"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The microbial assimilation of one-carbon (C1) gases is a topic of interest, given that products developed using this pathway have the potential to act as promising substrates for the synthesis of valuable chemicals via enzymatic oxidation or C\u2013C bonding. Despite extensive studies on C1 gas assimilation pathways, their key enzymes have yet to be subjected to high-throughput evolution studies on account of the lack of an efficient analytical tool for C1 metabolites. To address this challenging issue, we attempted to establish a fine-tuned single-cell\u2013level biosensor system constituting a combination of transcription factors (TFs) and several C1-converting enzymes that convert target compounds to the ligand of a TF. This enzymatic conversion broadens the detection range of ligands by the genetic biosensor systems. In this study, we presented new genetic enzyme screening systems (GESSs) to detect formate, formaldehyde, and methanol from specific enzyme activities and pathways, named FA-GESS, Frm-GESS, and MeOH-GESS, respectively. All the biosensors displayed linear responses to their respective C1 molecules, namely, formate (1.0\u2013250 mM), formaldehyde (1.0\u201350 \u03bcM), and methanol (5\u2013400 mM), and they did so with high specificity. Consequently, the helper enzymes, including formaldehyde dehydrogenase and methanol dehydrogenase, were successfully combined to constitute new versatile combinations of the C1-biosensors.<\/jats:p>","DOI":"10.3390\/ijms20092253","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T08:19:59Z","timestamp":1557389999000},"page":"2253","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["C1 Compound Biosensors: Design, Functional Study, and Applications"],"prefix":"10.3390","volume":"20","author":[{"given":"Jin-Young","family":"Lee","sequence":"first","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bong Hyun","family":"Sung","sequence":"additional","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"},{"name":"Department of Biosystems &amp; Bioengineering, KRIBB School of Biotechnology, University of Science &amp; Technology, Daejeon 34113, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So-Hyung","family":"Oh","sequence":"additional","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kil Koang","family":"Kwon","sequence":"additional","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2255-3418","authenticated-orcid":false,"given":"Hyewon","family":"Lee","sequence":"additional","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haseong","family":"Kim","sequence":"additional","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"},{"name":"Department of Biosystems &amp; Bioengineering, KRIBB School of Biotechnology, University of Science &amp; Technology, Daejeon 34113, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0423-9057","authenticated-orcid":false,"given":"Dae-Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"},{"name":"Department of Biosystems &amp; Bioengineering, KRIBB School of Biotechnology, University of Science &amp; Technology, Daejeon 34113, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7307-237X","authenticated-orcid":false,"given":"Soo-Jin","family":"Yeom","sequence":"additional","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4539-9812","authenticated-orcid":false,"given":"Seung-Goo","family":"Lee","sequence":"additional","affiliation":[{"name":"Synthetic Biology &amp; Bioengineering Research Center, KRIBB, Daejeon 34141, Korea"},{"name":"Department of Biosystems &amp; Bioengineering, KRIBB School of Biotechnology, University of Science &amp; Technology, Daejeon 34113, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4001","DOI":"10.1021\/es504242n","article-title":"Methane as a resource: Can the methanotrophs add value?","volume":"49","author":"Strong","year":"2015","journal-title":"Environ. 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