{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:39:59Z","timestamp":1790692799682,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T00:00:00Z","timestamp":1557792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CAPES-COFECUB","award":["ZIKANET project"],"award-info":[{"award-number":["ZIKANET project"]}]},{"name":"Research Federation BioST, Universit\u00e9 de La R\u00e9union","award":["ZIKAPAMIRE"],"award-info":[{"award-number":["ZIKAPAMIRE"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJMS"],"abstract":"<jats:p>Zika virus (ZIKV) and Dengue virus (DENV) are mosquito-borne viruses of the Flavivirus genus that could cause congenital microcephaly and hemorrhage, respectively, in humans, and thus present a risk to global public health. A preventive vaccine against ZIKV remains unavailable, and no specific antiviral drugs against ZIKV and DENV are licensed. Medicinal plants may be a source of natural antiviral drugs which mostly target viral entry. In this study, we evaluate the antiviral activity of Doratoxylum apetalum, an indigenous medicinal plant from the Mascarene Islands, against ZIKV and DENV infection. Our data indicated that D. apetalum exhibited potent antiviral activity against a contemporary epidemic strain of ZIKV and clinical isolates of four DENV serotypes at non-cytotoxic concentrations in human cells. Time-of-drug-addition assays revealed that D. apetalum extract acts on ZIKV entry by preventing the internalisation of virus particles into the host cells. Our data suggest that D. apetalum-mediated ZIKV inhibition relates to virus particle inactivation. We suggest that D. apetalum could be a promising natural source for the development of potential antivirals against medically important flaviviruses.<\/jats:p>","DOI":"10.3390\/ijms20102382","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T10:42:33Z","timestamp":1557830553000},"page":"2382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Doratoxylon apetalum, an Indigenous Medicinal Plant from Mascarene Islands, Is a Potent Inhibitor of Zika and Dengue Virus Infection in Human Cells"],"prefix":"10.3390","volume":"20","author":[{"given":"Juliano G.","family":"Haddad","sequence":"first","affiliation":[{"name":"Universit\u00e9 de la R\u00e9union, INSERM U1187, CNRS UMR 9192, IRD UMR 249, Unit\u00e9 Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, 94791 Sainte Clotilde, La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3542-1353","authenticated-orcid":false,"given":"Andrea Cristine","family":"Koishi","sequence":"additional","affiliation":[{"name":"Laboratorio de Virologia Molecular, Instituto Carlos Chagas, ICC\/FIOCRUZ\/PR, Curitiba, Parana 81350-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3648-7362","authenticated-orcid":false,"given":"Arnaud","family":"Gaudry","sequence":"additional","affiliation":[{"name":"Bioactive natural products unit, School of Pharmaceutical Sciences, EPGL, University of Geneva, Rue Michel-Servet 1, CH-1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Nunes Duarte dos Santos","sequence":"additional","affiliation":[{"name":"Laboratorio de Virologia Molecular, Instituto Carlos Chagas, ICC\/FIOCRUZ\/PR, Curitiba, Parana 81350-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0915-8635","authenticated-orcid":false,"given":"Wildriss","family":"Viranaicken","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de la R\u00e9union, INSERM U1187, CNRS UMR 9192, IRD UMR 249, Unit\u00e9 Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, 94791 Sainte Clotilde, La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8926-4050","authenticated-orcid":false,"given":"Philippe","family":"Despr\u00e8s","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de la R\u00e9union, INSERM U1187, CNRS UMR 9192, IRD UMR 249, Unit\u00e9 Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, 94791 Sainte Clotilde, La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4673-6614","authenticated-orcid":false,"given":"Chaker","family":"El Kalamouni","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de la R\u00e9union, INSERM U1187, CNRS UMR 9192, IRD UMR 249, Unit\u00e9 Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, 94791 Sainte Clotilde, La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1038\/s41467-019-09391-8","article-title":"Structure mapping of dengue and Zika viruses reveals functional long-range interactions","volume":"10","author":"Huber","year":"2019","journal-title":"Nat. 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