{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:46:53Z","timestamp":1790887613440,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T00:00:00Z","timestamp":1558051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The cloning, purification, and initial characterization of the \u03b2-carbonic anhydrase (CA, EC 4.2.1.1) from the genome of the opportunistic pathogen Malassezia restricta (MreCA), which a fungus involved in dandruff and seborrheic dermatitis (SD), is reported. MreCA is a protein consisting of 230 amino acid residues and shows high catalytic activity for the hydration of CO2 into bicarbonate and protons, with the following kinetic parameters: kcat of 1.06 \u00d7 106 s\u22121 and kcat\/KM of 1.07 \u00d7 108 M\u22121 s\u22121. It is also sensitive to inhibition by the sulfonamide acetazolamide (KI of 50.7 nM). Phylogenetically, MreCA and other CAs from various Malassezia species seem to be on a different branch, distinct from that of other \u03b2-CAs found in fungi, such as Candida spp., Saccharomyces cerevisiae, Aspergillus fumigatus, and Sordaria macrospora, with only Cryptococcus neoformans and Ustilago maydis enzymes clustering near MreCA. The further characterization of this enzyme and the identification of inhibitors that may interfere with its life cycle might constitute new strategies for fighting dandruff and SD.<\/jats:p>","DOI":"10.3390\/ijms20102447","type":"journal-article","created":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T11:06:46Z","timestamp":1558091206000},"page":"2447","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Cloning, Purification, and Characterization of a \u03b2-Carbonic Anhydrase from Malassezia restricta, an Opportunistic Pathogen Involved in Dandruff and Seborrheic Dermatitis"],"prefix":"10.3390","volume":"20","author":[{"given":"Sonia","family":"Del Prete","sequence":"first","affiliation":[{"name":"Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Vullo","sequence":"additional","affiliation":[{"name":"Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Universit\u00e0 degli Studi di Firenze, Via U. Schiff 6, 50019 Sesto Fiorentino, Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Ghobril","sequence":"additional","affiliation":[{"name":"L\u2019Or\u00e9al Research and Innovation, 93601 Aulnay-sous-Bois, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Hitce","sequence":"additional","affiliation":[{"name":"L\u2019Or\u00e9al Research and Innovation, 93601 Aulnay-sous-Bois, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Clavaud","sequence":"additional","affiliation":[{"name":"L\u2019Or\u00e9al Research and Innovation, 93601 Aulnay-sous-Bois, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Marat","sequence":"additional","affiliation":[{"name":"L\u2019Or\u00e9al Research and Innovation, 93601 Aulnay-sous-Bois, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3314-2411","authenticated-orcid":false,"given":"Clemente","family":"Capasso","sequence":"additional","affiliation":[{"name":"Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-0323","authenticated-orcid":false,"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Universit\u00e0 degli Studi di Firenze, Via U. Schiff 6, 50019 Sesto Fiorentino, Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4421","DOI":"10.1021\/cr200176r","article-title":"Multiple binding modes of inhibitors to carbonic anhydrases: How to design specific drugs targeting 15 different isoforms?","volume":"112","author":"Alterio","year":"2012","journal-title":"Chem. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1080\/17460441.2019.1567488","article-title":"Novel approaches for designing drugs that interfere with pH regulation","volume":"14","author":"Berrino","year":"2019","journal-title":"Expert Opin. 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