{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T13:47:56Z","timestamp":1788184076038,"version":"build-2803163510"},"reference-count":74,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T00:00:00Z","timestamp":1558656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Department of Education and Research","award":["PRIN 20158EB2CM"],"award-info":[{"award-number":["PRIN 20158EB2CM"]}]},{"name":"Italian Ministry of Health","award":["Ricerca Corrente (2017-2018)"],"award-info":[{"award-number":["Ricerca Corrente (2017-2018)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A number of factors can trigger amyotrophic lateral sclerosis (ALS), although its precise pathogenesis is still uncertain. In a previous study done by us, poisonous liquoral levels of hydrogen sulphide (H2S) in sporadic ALS patients were reported. In the same study very high concentrations of H2S in the cerebral tissues of the familial ALS (fALS) model of the SOD1G93A mouse, were measured. The objective of this study was to test whether decreasing the levels of H2S in the fALS mouse could be beneficial. Amino-oxyacetic acid (AOA)\u2014a systemic dual inhibitor of cystathionine-\u03b2-synthase and cystathionine-\u03b3 lyase (two key enzymes in the production of H2S)\u2014was administered to fALS mice. AOA treatment decreased the content of H2S in the cerebral tissues, and the lifespan of female mice increased by approximately ten days, while disease progression in male mice was not affected. The histological evaluation of the spinal cord of the females revealed a significant increase in GFAP positivity and a significant decrease in IBA1 positivity. In conclusion, the results of the study indicate that, in the animal model, the inhibition of H2S production is more effective in females. The findings reinforce the need to adequately consider sex as a relevant factor in ALS.<\/jats:p>","DOI":"10.3390\/ijms20102550","type":"journal-article","created":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T11:20:46Z","timestamp":1558696846000},"page":"2550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Impact of Pharmacological Inhibition of Hydrogen Sulphide Production in the SOD1G93A-ALS Mouse Model"],"prefix":"10.3390","volume":"20","author":[{"given":"Alida","family":"Spalloni","sequence":"first","affiliation":[{"name":"Department of Experimental Neuroscience, Molecular Neurobiology Unit, IRCCS Fondazione Santa Lucia, 00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Greco","sequence":"additional","affiliation":[{"name":"Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"},{"name":"Institute of Biochemistry and Clinical Biochemistry, Universit\u00e0 Cattolica del Sacro Cuore, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4896-803X","authenticated-orcid":false,"given":"Giulia","family":"Ciriminna","sequence":"additional","affiliation":[{"name":"Department of Experimental Neuroscience, Molecular Neurobiology Unit, IRCCS Fondazione Santa Lucia, 00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Corasolla Carregari","sequence":"additional","affiliation":[{"name":"Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"},{"name":"Institute of Biochemistry and Clinical Biochemistry, Universit\u00e0 Cattolica del Sacro Cuore, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Marini","sequence":"additional","affiliation":[{"name":"Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"},{"name":"Institute of Biochemistry and Clinical Biochemistry, Universit\u00e0 Cattolica del Sacro Cuore, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7141-1817","authenticated-orcid":false,"given":"Luisa","family":"Pieroni","sequence":"additional","affiliation":[{"name":"Department of Experimental Neuroscience, Proteomics and Metabonomics Unit, IRCCS Fondazione Santa Lucia, 00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola B.","family":"Mercuri","sequence":"additional","affiliation":[{"name":"Department of Systems Medicine, Policlinico Universitario \u201cTor Vergata\u201d, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"},{"name":"Department of Experimental Neuroscience, Experimental Neurology Unit, IRCCS Fondazione Santa Lucia, 00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Urbani","sequence":"additional","affiliation":[{"name":"Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"},{"name":"Institute of Biochemistry and Clinical Biochemistry, Universit\u00e0 Cattolica del Sacro Cuore, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Longone","sequence":"additional","affiliation":[{"name":"Department of Experimental Neuroscience, Molecular Neurobiology Unit, IRCCS Fondazione Santa Lucia, 00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1136\/jnnp-2014-308946","article-title":"The expanding syndrome of amyotrophic lateral sclerosis: A clinical and molecular odyssey","volume":"86","author":"Turner","year":"2015","journal-title":"J. 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