{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:15:05Z","timestamp":1760188505864,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T00:00:00Z","timestamp":1559606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["271604"],"award-info":[{"award-number":["271604"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601222"],"award-info":[{"award-number":["81601222"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To unravel the mechanisms behind the higher resistance to light damage of juvenile (JR) versus adult (AR) rats, Sprague Dawley rats were exposed to a bright luminous environment of 10, 000 lux. The light-induced retinopathy (LIR) was assessed with histology, electroretinography and immunohistochemistry (IHC). In JR, 2 days of exposure induced the typical LIR, while &gt;3 days added little LIR. IHC revealed a subtle migration of microglia (Iba1 marker) from the inner to the outer retina after 3 days of exposure in JR contrasting with the stronger reaction seen after 1 day in AR. Similarly, in JR, the M\u00fcller cells expressed less intense GFAP, CNTF and FGF2 staining compared to AR. Our results suggest that in JR the degree of retinal damage is not proportional to the duration of light exposure (i.e., dose-independent retinopathy), contrasting with the dose-dependent LIR reported in AR. The immature immune system in JR may explain the delayed and\/or weaker inflammatory response compared to AR, a finding that would also point to the devastating contribution of the immune system in generating the LIR phenotype, a claim also advanced to explain the pathophysiology of other retinal degenerative disorders such as Age-related Macular Degeneration, Diabetic Retinopathy and Retinitis Pigmentosa.<\/jats:p>","DOI":"10.3390\/ijms20112744","type":"journal-article","created":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T09:37:58Z","timestamp":1559727478000},"page":"2744","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evidences Suggesting that Distinct Immunological and Cellular Responses to Light Damage Distinguishes Juvenile and Adult Rat Retinas"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7233-0138","authenticated-orcid":false,"given":"Anna","family":"Polosa","sequence":"first","affiliation":[{"name":"Dept of Ophthalmology &amp; Neurology-Neurosurgery, McGill University\/Montreal Children\u2019s Hospital Research Institute, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shasha","family":"Lv","sequence":"additional","affiliation":[{"name":"Dept of Ophthalmology &amp; Neurology-Neurosurgery, McGill University\/Montreal Children\u2019s Hospital Research Institute, Montr\u00e9al, QC H4A 3J1, Canada"},{"name":"Health Science Center, Xi\u2019an Jiaotong University, 76 West Yanta Road, Xi\u2019an 710061, Shaanxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wassila","family":"Ait Igrine","sequence":"additional","affiliation":[{"name":"Dept of Ophthalmology &amp; Neurology-Neurosurgery, McGill University\/Montreal Children\u2019s Hospital Research Institute, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura-Alexie","family":"Chevrolat","sequence":"additional","affiliation":[{"name":"Dept of Ophthalmology &amp; Neurology-Neurosurgery, McGill University\/Montreal Children\u2019s Hospital Research Institute, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyba","family":"Bessaklia","sequence":"additional","affiliation":[{"name":"Dept of Ophthalmology &amp; Neurology-Neurosurgery, McGill University\/Montreal Children\u2019s Hospital Research Institute, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Lachapelle","sequence":"additional","affiliation":[{"name":"Dept of Ophthalmology &amp; Neurology-Neurosurgery, McGill University\/Montreal Children\u2019s Hospital Research Institute, Montr\u00e9al, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,4]]},"reference":[{"key":"ref_1","first-page":"782","article-title":"Extreme retinal remodeling triggered by light damage: Implications for age related macular degeneration","volume":"14","author":"Marc","year":"2008","journal-title":"Mol. Vis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.3109\/02713681003682925","article-title":"Brief exposure to damaging light causes focal recruitment of macrophages, and long-term destabilization of photoreceptors in the albino rat retina","volume":"35","author":"Rutar","year":"2010","journal-title":"Curr. Eye Res."},{"key":"ref_3","first-page":"450","article-title":"Retinal damage by light in rats","volume":"5","author":"Noell","year":"1966","journal-title":"Invest. Ophthalmol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3202","DOI":"10.1167\/iovs.05-1515","article-title":"Light-induced retinopathy: Comparing adult and juvenile rats","volume":"47","author":"Joly","year":"2006","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.exer.2008.06.002","article-title":"Topography of retinal damage in light-exposed albino rats","volume":"87","author":"Tanito","year":"2008","journal-title":"Exp. Eye Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Polosa, A., Liu, W., and Lachapelle, P. (2016). Retinotopic distribution of structural and functional damages following bright light exposure of juvenile rats. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146979"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1167\/iovs.06-1205","article-title":"Neuroprotection in the juvenile rat model of light-induced retinopathy: Evidence suggesting a role for fgf-2 and cntf","volume":"48","author":"Joly","year":"2007","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9282","DOI":"10.1523\/JNEUROSCI.18-22-09282.1998","article-title":"Intraocular gene transfer of ciliary neurotrophic factor prevents death and increases responsiveness of rod photoreceptors in the retinal degeneration slow mouse","volume":"18","author":"Cayouette","year":"1998","journal-title":"J. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"9228","DOI":"10.1523\/JNEUROSCI.22-21-09228.2002","article-title":"Microglia-muller glia cell interactions control neurotrophic factor production during light-induced retinal degeneration","volume":"22","author":"Harada","year":"2002","journal-title":"J. Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0014-4835(02)00332-9","article-title":"Activated microglia in human retinitis pigmentosa, late-onset retinal degeneration, and age-related macular degeneration","volume":"76","author":"Gupta","year":"2003","journal-title":"Exp. Eye Res."},{"key":"ref_11","first-page":"503725","article-title":"Infiltration of proinflammatory m1 macrophages into the outer retina precedes damage in a mouse model of age-related macular degeneration","volume":"2013","author":"Saeed","year":"2013","journal-title":"Int. J. Inflam."},{"key":"ref_12","first-page":"3799","article-title":"Light-induced degeneration and microglial response in the retina of an epibenthonic pigmented teleost: Age-dependent photoreceptor susceptibility to cell death","volume":"215","author":"Blasco","year":"2012","journal-title":"J. Exp. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1002\/jnr.10253","article-title":"Cytokines, chemokines, and cytokine receptors in human microglia","volume":"69","author":"Lee","year":"2002","journal-title":"J. Neurosci. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1126\/science.996550","article-title":"Cones survive rods in the light-damaged eye of the albino rat","volume":"194","author":"Cicerone","year":"1976","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1002\/aja.1001270305","article-title":"The development of the retina of the albino rat","volume":"127","author":"Braekevelt","year":"1970","journal-title":"Am. J. Anat."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1167\/iovs.07-0916","article-title":"Early manifestations of postnatal hyperoxia on the retinal structure and function of the neonatal rat","volume":"49","author":"Dorfman","year":"2008","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1080\/02713683.2016.1255336","article-title":"Light-induced retinopathy: Young age protects more than ocular pigmentation","volume":"42","author":"Polosa","year":"2017","journal-title":"Curr. Eye Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Polosa, A., Bessaklia, H., and Lachapelle, P. (2016). Strain differences in light-induced retinopathy. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0158082"},{"key":"ref_19","first-page":"442","article-title":"The photopic electroretinogram in congenital stationary night blindness with myopia","volume":"24","author":"Lachapelle","year":"1983","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/S1350-9462(98)00006-8","article-title":"Oscillatory potentials in the retina: What do they reveal","volume":"17","author":"Wachtmeister","year":"1998","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Heckenlively, J.R., and Arden, G.B. (2006). The oscillatory potentials of the electroretinogram. Principles and Practice of Clinical Electrophysiology of Vision, The MIT Press. Chapter 43.","DOI":"10.7551\/mitpress\/5557.003.0060"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1002\/cne.902630407","article-title":"Cellular degeneration and synaptogenesis in the developing retina of the rat","volume":"263","author":"Horsburgh","year":"1987","journal-title":"J. Comp. Neurol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Rapp, L., and Williams, T. (1980). A parametric study of retinal light damage in albino and pigmented rats. The Effects of Constant Light on Visual Processes, Springer.","DOI":"10.1007\/978-1-4684-7257-8_6"},{"key":"ref_24","first-page":"3511","article-title":"DNA damage and repair in light-induced photoreceptor degeneration","volume":"43","author":"Gordon","year":"2002","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"ref_25","first-page":"765","article-title":"Expression of ccl2 and its receptor in activation and migration of microglia and monocytes induced by photoreceptor apoptosis","volume":"23","author":"Feng","year":"2017","journal-title":"Mol. Vis."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Jiao, H., Natoli, R., Valter, K., Provis, J.M., and Rutar, M. (2015). Spatiotemporal cadence of macrophage polarisation in a model of light-induced retinal degeneration. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0143952"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"17897","DOI":"10.1038\/s41598-017-18236-7","article-title":"Involvement of innate immune system in late stages of inherited photoreceptor degeneration","volume":"7","author":"Sudharsan","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"eaap8492","DOI":"10.1126\/sciadv.aap8492","article-title":"Repopulating retinal microglia restore endogenous organization and function under cx3cl1-cx3cr1 regulation","volume":"4","author":"Zhang","year":"2018","journal-title":"Sci. Adv."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1023\/A:1022511918823","article-title":"Dark adaptation is faster in pigmented than albino rats","volume":"106","author":"Behn","year":"2003","journal-title":"Doc. Ophthalmol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1167\/iovs.10-6197","article-title":"Immunohistochemical evidence of synaptic retraction, cytoarchitectural remodeling, and cell death in the inner retina of the rat model of oygen-induced retinopathy (oir)","volume":"52","author":"Dorfman","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/11\/2744\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:56:05Z","timestamp":1760187365000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/11\/2744"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,4]]},"references-count":30,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2019,6]]}},"alternative-id":["ijms20112744"],"URL":"https:\/\/doi.org\/10.3390\/ijms20112744","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2019,6,4]]}}}