{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:14:27Z","timestamp":1789535667220,"version":"build-2803163510"},"reference-count":76,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,6,24]],"date-time":"2019-06-24T00:00:00Z","timestamp":1561334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["19-12338S"],"award-info":[{"award-number":["19-12338S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004240","name":"Akademie V\u011bd \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["MSM200201702"],"award-info":[{"award-number":["MSM200201702"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Bordetella pertussis is a Gram-negative strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Previously, we have shown that RNA chaperone Hfq is required for virulence of B. pertussis. Furthermore, microarray analysis revealed that a large number of genes are affected by the lack of Hfq. This study represents the first attempt to characterize the Hfq regulon in bacterial pathogen using an integrative omics approach. Gene expression profiles were analyzed by RNA-seq and protein amounts in cell-associated and cell-free fractions were determined by LC-MS\/MS technique. Comparative analysis of transcriptomic and proteomic data revealed solid correlation (r2 = 0.4) considering the role of Hfq in post-transcriptional control of gene expression. Importantly, our study confirms and further enlightens the role of Hfq in pathogenicity of B. pertussis as it shows that \u0394hfq strain displays strongly impaired secretion of substrates of Type III secretion system (T3SS) and substantially reduced resistance to serum killing. On the other hand, significantly increased production of proteins implicated in transport of important metabolites and essential nutrients observed in the mutant seems to compensate for the physiological defect introduced by the deletion of the hfq gene.<\/jats:p>","DOI":"10.3390\/ijms20123073","type":"journal-article","created":{"date-parts":[[2019,6,24]],"date-time":"2019-06-24T02:37:40Z","timestamp":1561343860000},"page":"3073","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Comparative Integrated Omics Analysis of the Hfq Regulon in Bordetella pertussis"],"prefix":"10.3390","volume":"20","author":[{"given":"Ana","family":"Dienstbier","sequence":"first","affiliation":[{"name":"Laboratory of post-transcriptional control of gene expression, Institute of Microbiology v.v.i., 14220 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8646-859X","authenticated-orcid":false,"given":"Fabian","family":"Amman","sequence":"additional","affiliation":[{"name":"Institute for Theoretical Chemistry, University of Vienna, W\u00e4hringer Stra\u00dfe 17, A-1090 Vienna, Austria"},{"name":"Division of Cell and Developmental Biology, Medical University of Vienna, Schwarzspanierstra\u00dfe 17, A-1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"\u0160tipl","sequence":"additional","affiliation":[{"name":"Laboratory of post-transcriptional control of gene expression, Institute of Microbiology v.v.i., 14220 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denisa","family":"Petr\u00e1\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of post-transcriptional control of gene expression, Institute of Microbiology v.v.i., 14220 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branislav","family":"Ve\u010derek","sequence":"additional","affiliation":[{"name":"Laboratory of post-transcriptional control of gene expression, Institute of Microbiology v.v.i., 14220 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1128\/CMR.18.2.326-382.2005","article-title":"Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to bordetella pertussis and other bordetella subspecies","volume":"18","author":"Mattoo","year":"2005","journal-title":"Clin. 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