{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T11:20:36Z","timestamp":1769599236794,"version":"3.49.0"},"reference-count":46,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2015\/19\/N\/NZ5\/02257"],"award-info":[{"award-number":["2015\/19\/N\/NZ5\/02257"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We aimed to identify differences in mutational status between follicular thyroid adenoma (FTA) and follicular thyroid cancer (FTC). The study included 35 patients with FTA and 35 with FTC. DNA was extracted from formalin-fixed paraffin-embedded (FFPE) samples from thyroidectomy. Next-generation sequencing (NGS) was performed with the 50-gene Ion AmpliSeq Cancer Hotspot Panel v2. Potentially pathogenic mutations were found in 14 (40%) FTA and 24 (69%) FTC patients (OR (95%CI) = 3.27 (1.22\u22128.75)). The number of mutations was higher in patients with FTC than FTA (p-value = 0.03). SMAD4 and STK11 mutations were present only in patients with FTA, while defects in FBXW7, JAK3, KIT, NRAS, PIK3CA, SMARCB1, and TP53 were detected exclusively in FTC patients. TP53 mutations increased the risk of FTC; OR (95%CI) = 29.24 (1.64\u2013522.00); p-value = 0.001. FLT3-positivity was higher in FTC than in the FTA group (51.4% vs. 28.6%; p-value = 0.051). The presence of FLT3 and TP53 with no RET mutations increased FTC detectability by 17.1%, whereas the absence of FLT3 and TP53 with a presence of RET mutations increased FTA detectability by 5.7%. TP53 and FLT3 are candidate markers for detecting malignancy in follicular lesions. The best model to predict FTA and FTC may consist of FLT3, TP53, and RET mutations considered together.<\/jats:p>","DOI":"10.3390\/ijms20133126","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T02:43:41Z","timestamp":1561603421000},"page":"3126","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Differences in Mutational Profile between Follicular Thyroid Carcinoma and Follicular Thyroid Adenoma Identified Using Next Generation Sequencing"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4824-8131","authenticated-orcid":false,"given":"Martyna","family":"Borowczyk","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Diseases, Poznan University of Medical Sciences, 60-355 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewelina","family":"Szczepanek-Parulska","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Diseases, Poznan University of Medical Sciences, 60-355 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Szymon","family":"D\u0119bicki","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Diseases, Poznan University of Medical Sciences, 60-355 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart\u0142omiej","family":"Budny","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Diseases, Poznan University of Medical Sciences, 60-355 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederik A.","family":"Verburg","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, University Hospital Marburg, 35043 Marburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"Filipowicz","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Diseases, Poznan University of Medical Sciences, 60-355 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1811-2583","authenticated-orcid":false,"given":"Barbara","family":"Wi\u0119ckowska","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Statistics, Poznan University of Medical Sciences, 60-806 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u0142gorzata","family":"Janicka-Jedy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Poznan University of Medical Sciences, 60-355 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidia","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Hematology and Bone Marrow Transplantation, Poznan University of Medical Sciences, 60-569 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5377-8834","authenticated-orcid":false,"given":"Katarzyna","family":"Ziemnicka","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Diseases, Poznan University of Medical Sciences, 60-355 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6296-7220","authenticated-orcid":false,"given":"Marek","family":"Rucha\u0142a","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Diseases, Poznan University of Medical Sciences, 60-355 Pozna\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1634\/theoncologist.2010-0405","article-title":"Follicular adenoma and carcinoma of the thyroid gland","volume":"16","author":"McHenry","year":"2011","journal-title":"Oncologist"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/s12943-018-0866-1","article-title":"Thyroid cancers of follicular origin in a genomic light: In-depth overview of common and unique molecular marker candidates","volume":"17","author":"Pstrag","year":"2018","journal-title":"Mol. 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