{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:59:51Z","timestamp":1792184391225,"version":"4.4.0"},"reference-count":78,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Forage legumes are an important livestock nutritional resource, which includes essential metals, such as copper. Particularly, the high prevalence of hypocuprosis causes important economic losses to Argentinian cattle agrosystems. Copper deficiency in cattle is partially due to its low content in forage produced by natural grassland, and is exacerbated by flooding conditions. Previous results indicated that incorporation of Lotus spp. into natural grassland increases forage nutritional quality, including higher copper levels. However, the biological processes and molecular mechanisms involved in copper uptake by Lotus spp. remain poorly understood. Here, we identify four genes that encode putative members of the Lotus copper transporter family, denoted COPT in higher plants. A heterologous functional complementation assay of the Saccharomyces cerevisiae ctr1\u2206ctr3\u2206 strain, which lacks the corresponding yeast copper transporters, with the putative Lotus COPT proteins shows a partial rescue of the yeast phenotypes in restrictive media. Under partial submergence conditions, the copper content of L. japonicus plants decreases and the expression of two Lotus COPT genes is induced. These results strongly suggest that the Lotus COPT proteins identified in this work function in copper uptake. In addition, the fact that environmental conditions affect the expression of certain COPT genes supports their involvement in adaptive mechanisms and envisages putative biotechnological strategies to improve cattle copper nutrition.<\/jats:p>","DOI":"10.3390\/ijms20133136","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T08:19:13Z","timestamp":1561623553000},"page":"3136","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of the Copper Transporters from Lotus spp. and Their Involvement under Flooding Conditions"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7413-3541","authenticated-orcid":false,"given":"Francisco J.","family":"Escaray","sequence":"first","affiliation":[{"name":"Instituto Tecnol\u00f3gico de Chascom\u00fas (INTECh), UNSAM\/CONICET, Avda. Intendente Marino Km. 8.2, Chascom\u00fas, Buenos Aires 7130, Argentina"},{"name":"Departament de Bioqu\u00edmica i Biologia Molecular, Estructura de Recerca Interdisciplinar en Biotecnologiaia i Biomedicina (ERI BIOTECMED), Universitat de Val\u00e8ncia. Burjassot, 46100 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian J.","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Instituto Tecnol\u00f3gico de Chascom\u00fas (INTECh), UNSAM\/CONICET, Avda. Intendente Marino Km. 8.2, Chascom\u00fas, Buenos Aires 7130, Argentina"},{"name":"Instituto de Fisiolog\u00eda Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), La Plata, Buenos Aires 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo J.","family":"Copello","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u0131\u0301mica y Metabolismo del F\u00e1rmaco (IQUIMEFA), Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Ciudad Aut\u00f3noma de Buenos Aires, Buenos Aires C113AAD, Argentina"},{"name":"Departamento de Qu\u00edmica Anal\u00edtica y Fisicoqu\u00edmica, Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires (UBA), Ciudad Aut\u00f3noma de Buenos Aires, Buenos Aires C113AAD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergi","family":"Puig","sequence":"additional","affiliation":[{"name":"Instituto de Agroqu\u00edmica y Tecnolog\u00eda de los Alimentos, Centro Superior de Investigaciones Cient\u00edficas, IATA-CSIC, Paterna, 46980 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lola","family":"Pe\u00f1arrubia","sequence":"additional","affiliation":[{"name":"Departament de Bioqu\u00edmica i Biologia Molecular, Estructura de Recerca Interdisciplinar en Biotecnologiaia i Biomedicina (ERI BIOTECMED), Universitat de Val\u00e8ncia. Burjassot, 46100 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar A.","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Instituto Tecnol\u00f3gico de Chascom\u00fas (INTECh), UNSAM\/CONICET, Avda. Intendente Marino Km. 8.2, Chascom\u00fas, Buenos Aires 7130, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Perea-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Tecnol\u00f3gico de Chascom\u00fas (INTECh), UNSAM\/CONICET, Avda. Intendente Marino Km. 8.2, Chascom\u00fas, Buenos Aires 7130, Argentina"},{"name":"Instituto de Agroqu\u00edmica y Tecnolog\u00eda de los Alimentos, Centro Superior de Investigaciones Cient\u00edficas, IATA-CSIC, Paterna, 46980 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,6,27]]},"reference":[{"key":"ref_1","unstructured":"Marschner, P. (2012). 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