{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:13:35Z","timestamp":1790511215105,"version":"4.1.0"},"reference-count":67,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2019,7,14]],"date-time":"2019-07-14T00:00:00Z","timestamp":1563062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>(1) In vitro, bone marrow-derived stromal cells (BMSCs) demonstrate inter-donor phenotypic variability, which presents challenges for the development of regenerative therapies. Here, we investigated whether the frequency of putative BMSC sub-populations within the freshly isolated mononuclear cell fraction of bone marrow is phenotypically predictive for the in vitro derived stromal cell culture. (2) Vertebral body, iliac crest, and femoral head bone marrow were acquired from 33 patients (10 female and 23 male, age range 14\u201391). BMSC sub-populations were identified within freshly isolated mononuclear cell fractions based on cell-surface marker profiles. Stromal cells were expanded in monolayer on tissue culture plastic. Phenotypic assessment of in vitro derived cell cultures was performed by examining growth kinetics, chondrogenic, osteogenic, and adipogenic differentiation. (3) Gender, donor age, and anatomical site were neither predictive for the total yield nor the population doubling time of in vitro derived BMSC cultures. The abundance of freshly isolated progenitor sub-populations (CD45\u2212CD34\u2212CD73+, CD45\u2212CD34\u2212CD146+, NG2+CD146+) was not phenotypically predictive of derived stromal cell cultures in terms of growth kinetics nor plasticity. BMSCs derived from iliac crest and vertebral body bone marrow were more responsive to chondrogenic induction, forming superior cartilaginous tissue in vitro, compared to those isolated from femoral head. (4) The identification of discrete progenitor populations in bone marrow by current cell-surface marker profiling is not predictive for subsequently derived in vitro BMSC cultures. Overall, the iliac crest and the vertebral body offer a more reliable tissue source of stromal progenitor cells for cartilage repair strategies compared to femoral head.<\/jats:p>","DOI":"10.3390\/ijms20143454","type":"journal-article","created":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T04:55:27Z","timestamp":1563166527000},"page":"3454","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Phenotypic Characterization of Bone Marrow Mononuclear Cells and Derived Stromal Cell Populations from Human Iliac Crest, Vertebral Body and Femoral Head"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2412-7474","authenticated-orcid":false,"given":"Marietta","family":"Herrmann","sequence":"first","affiliation":[{"name":"AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland"},{"name":"IZKF Group Tissue Regeneration in Musculoskeletal Diseases, University Clinics Wuerzburg, 97070 Wuerzburg, Germany"},{"name":"Orthopedic Center for Musculoskeletal Research, University of Wuerzburg, 97076 Wuerzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Hildebrand","sequence":"additional","affiliation":[{"name":"AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Menzel","sequence":"additional","affiliation":[{"name":"AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niamh","family":"Fahy","sequence":"additional","affiliation":[{"name":"AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland"},{"name":"Department of Orthopaedics, Erasmus MC, University Medical Center Rotterdam, 3015 Rotterdam, The Netherlands"},{"name":"Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical Center Rotterdam, 3015 Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Alini","sequence":"additional","affiliation":[{"name":"AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siegmund","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery, Regensburg University Medical Center, 93053 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorin","family":"Benneker","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery and Traumatology, Spine Unit, Inselspital, Bern University Hospital, 3010 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2478-2804","authenticated-orcid":false,"given":"Sophie","family":"Verrier","sequence":"additional","affiliation":[{"name":"AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9538-1517","authenticated-orcid":false,"given":"Martin J.","family":"Stoddart","sequence":"additional","affiliation":[{"name":"AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer J.","family":"Bara","sequence":"additional","affiliation":[{"name":"AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland"},{"name":"Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1242\/dev.16.3.381","article-title":"Osteogenesis in transplants of bone marrow cells","volume":"16","author":"Friedenstein","year":"1966","journal-title":"Development"},{"key":"ref_2","first-page":"267","article-title":"Fibroblast precursors in normal and irradiated mouse hematopoietic organs","volume":"4","author":"Friedenstein","year":"1976","journal-title":"Exp. 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