{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T03:19:20Z","timestamp":1776136760691,"version":"3.50.1"},"reference-count":56,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T00:00:00Z","timestamp":1566345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["6200020055A"],"award-info":[{"award-number":["6200020055A"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Snake venom contains large amounts of active proteins and peptides. In this study, a novel snake protein, metalloproteinase SP, was successfully isolated from the venom of Agkistrodon acutus by multi-gel chromatography. The isolated protein exhibits anti-platelet aggregation activity. Animal experiments showed that it exhibited defibration, anticoagulation, and antithrombotic effects and contributes to improved blood rheology and antiplatelet aggregation. In vivo experiments demonstrated that it prolonged clotting time, partial thromboplastin time, prothrombin time, thrombin time, fibrinogen time and reduced fibrinogen content of mice. Also, metalloproteinase SP inhibited carrageenan-induced tail thrombosis, ADP-induced acute pulmonary embolism, and ADP, Arachidonic acid (AA), or collagen-induced platelet aggregation. In vitro experiments showed that the protein cleaved the \u03b1, \u03b2, and \u03b3 chains of fibrinogen. Metabolomic analysis upon metalloproteinase SP treatment revealed that 14 metabolites, which are mainly involved in phenylalanine, tyrosine, and tryptophan biosynthesis, responded to metalloproteinase SP treatment. In summary, the isolated snake venom protein inhibits formation of acute pulmonary embolism probably through regulating and restoring perturbed energy, lipid, and amino acid metabolism.<\/jats:p>","DOI":"10.3390\/ijms20174088","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T11:19:06Z","timestamp":1566386346000},"page":"4088","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Isolation of a Novel Metalloproteinase from Agkistrodon Venom and Its Antithrombotic Activity Analysis"],"prefix":"10.3390","volume":"20","author":[{"given":"Jin","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojian","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China"},{"name":"Chinese Medicine College, China Pharmaceutical University, Nanjing 210009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China"},{"name":"Chinese Medicine College, China Pharmaceutical University, Nanjing 210009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/j.thromres.2014.08.014","article-title":"Thrombosis: A major contributor to global disease burden","volume":"134","author":"Raskob","year":"2014","journal-title":"Thromb. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4115","DOI":"10.1113\/jphysiol.2011.212886","article-title":"Ischaemic stroke: A thrombo-inflammatory disease","volume":"589","author":"Nieswandt","year":"2011","journal-title":"J. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1182\/blood-2014-03-562876","article-title":"Aspirin-triggered 15-epi-lipoxin A 4 regulates neutrophil-platelet aggregation and attenuates acute lung injury in mice","volume":"124","author":"Mallavia","year":"2014","journal-title":"Blood"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1146\/annurev.genom.9.081307.164356","article-title":"The toxicogenomic multiverse: Convergent recruitment of proteins into animal venoms","volume":"10","author":"Fry","year":"2009","journal-title":"Annu. Rev. Genom. Hum. G."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1177\/0300060514521154","article-title":"Absence of association between atherosclerotic cerebral infarction and TNFSF4\/TNFRSF4 single nucleotide polymorphisms rs1234313, rs1234314 and rs17568 in a Chinese population","volume":"42","author":"Huang","year":"2014","journal-title":"J. Int. Med. Res."},{"key":"ref_6","first-page":"2","article-title":"Thrombin-like enzymes in snake venoms","volume":"Volume 39","author":"Kini","year":"2010","journal-title":"Toxins and Hemostasis"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2793","DOI":"10.1182\/blood.V96.8.2793","article-title":"Analysis of fibrin formation and proteolysis during intravenous administration of ancrod","volume":"96","author":"Dempfle","year":"2000","journal-title":"Blood"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Bocian, A., Urbanik, M., Hus, K., \u0141yskowski, A., Petrilla, V., Andrej\u010d\u00e1kov\u00e1, Z., Petrillov\u00e1, M., and Leg\u00e1th, J. (2016). Proteomic analyses of Agkistrodon contortrix venom using 2D electrophoresis and MS techniques. Toxins, 8.","DOI":"10.3390\/toxins8120372"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jprot.2013.10.036","article-title":"Venomics of new world pit vipers: Genus-wide comparisons of venom proteomes across Agkistrodon","volume":"96","author":"Lomonte","year":"2014","journal-title":"J. Proteomics"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.ejps.2013.02.002","article-title":"Biochemical properties and comparative pharmacology of a coagulant from Deinagkistrodon acutus snake venom","volume":"49","author":"Tang","year":"2013","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_11","first-page":"1","article-title":"Antiplatelet aggregation and antithrombosis efficiency of peptides in the snake venom of Deinagkistrodon acutusr, isolation, identification, and evaluation","volume":"5","author":"Ding","year":"2015","journal-title":"Evid-Based Compl. Alt."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"10846","DOI":"10.1038\/srep10846","article-title":"A novel direct factor Xa inhibitory peptide with anti-platelet aggregation activity from Agkistrodon acutus venom hydrolysates","volume":"5","author":"Ming","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1007\/s00204-012-0957-5","article-title":"Crystal structure and activating effect on RyRs of AhV_TL-I, a glycosylated thrombin-like enzyme from Agkistrodon halys snake venom","volume":"87","author":"Zeng","year":"2013","journal-title":"Arch. Toxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1007\/s00018-003-3325-z","article-title":"Snake venom thrombin-like enzymes: From reptilase to now","volume":"61","author":"Castro","year":"2004","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ge, Y.H., Chen, Y.Y., Zhou, G.S., Liu, X., Tang, Y.P., Liu, R., Liu, P., Li, N., Yang, J., and Wang, J. (2018). Novel antithrombotic protease from marine worm sipunculus nudus. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19103023"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1042\/BJ20060302","article-title":"Anticoagulant proteins from snake venoms: Structure, function and mechanism","volume":"397","author":"Kini","year":"2006","journal-title":"Biochem. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.toxicon.2010.05.012","article-title":"Comparative study of anticoagulant and procoagulant properties of 28 snake venoms from families Elapidae, Viperidae, and purified Russell\u2019s viper venom-factor X activator (RVV-X)","volume":"56","author":"Suntravat","year":"2010","journal-title":"Toxicon"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1146\/annurev-pharmtox-010814-124438","article-title":"New approaches to inhibiting platelets and coagulation","volume":"55","author":"Depta","year":"2015","journal-title":"Annu. Rev. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3030","DOI":"10.1007\/s00018-006-6315-0","article-title":"Snake venom components and their applications in biomedicine","volume":"63","author":"Koh","year":"2006","journal-title":"Cell Mol. Life Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1016\/S0076-6879(73)27039-8","article-title":"Automated edman degradation: The protein sequenator","volume":"27","author":"Niall","year":"1973","journal-title":"Method Enzymol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/00004311-200404230-00003","article-title":"Basics of coagulation pathways","volume":"42","author":"Breen","year":"2004","journal-title":"Int. Anesthesiol. Clin."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Corte, A.L.C.L., Philippou, H., and Ari\u00ebns, R.A.S. (2011). Role of fibrin structure in thrombosis and vascular disease. Adv. Protein Chem. Str., 75\u2013127.","DOI":"10.1016\/B978-0-12-381262-9.00003-3"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4609","DOI":"10.1182\/blood-2010-07-297671","article-title":"Visualization and identification of the structures formed during early stages of fibrin polymerization","volume":"117","author":"Chernysh","year":"2011","journal-title":"Blood"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1046\/j.1365-2141.1997.8532481.x","article-title":"Blood viscosity and risk of cardiovascular events: The Edinburgh Artery Study","volume":"96","author":"Lowe","year":"2015","journal-title":"Brit. J. Haematol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4176","DOI":"10.1182\/blood-2005-05-2180","article-title":"Genetic variation in the fibrinogen gamma gene increases the risk for deep venous thrombosis by reducing plasma fibrinogen gamma\u2019 levels","volume":"106","author":"Rosendaal","year":"2005","journal-title":"Blood"},{"key":"ref_26","first-page":"27","article-title":"Red blood cell rheological properties in various rat hypertension models","volume":"26","author":"Hacioglu","year":"2002","journal-title":"Clin. Hemorheol. Micro."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/j.1749-6632.2001.tb03521.x","article-title":"Platelet-fibrinogen interactions","volume":"936","author":"Bennett","year":"2010","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.bpc.2004.07.029","article-title":"The mechanical properties of fibrin for basic scientists and clinicians","volume":"112","author":"Weisel","year":"2004","journal-title":"Biophys. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1161\/01.ATV.0000241589.52950.4c","article-title":"Altered fibrin architecture is associated with hypofibrinolysis and premature coronary atherothrombosis","volume":"26","author":"Collet","year":"2006","journal-title":"Arterioscl. Throm. Vas."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1160\/TH07-06-0427","article-title":"Fibrinogen Hershey IV: A novel dysfibrinogen with a gammaV411I mutation in the integrin alpha(IIb)beta(3) binding site","volume":"99","author":"Collet","year":"2008","journal-title":"J. Thromb. Haemost."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"34812","DOI":"10.1074\/jbc.M306504200","article-title":"Integrin alpha IIb beta 3-dependent calcium signals regulate platelet-fibrinogen interactions under flow involvement of phospholipase C gamma 2","volume":"278","author":"Goncalves","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1097\/00001721-200209000-00008","article-title":"Analyzing fibrin clot structure using a microplate reader","volume":"13","author":"Wolberg","year":"2002","journal-title":"Blood Coagul Fibrin."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1111\/j.1538-7836.2007.02406.x","article-title":"Elevated plasma fibrinogen gamma\u2019 concentration is associated with myocardial infarction: Effects of variation in fibrinogen genes and environmental factors","volume":"5","author":"Mannila","year":"2010","journal-title":"J. Thromb. Haemost."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1055\/s-0038-1648479","article-title":"Blood viscosity and cardiovascular disease","volume":"67","author":"Lowe","year":"1992","journal-title":"J. Thromb. Haemost."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0021-9150(92)90234-8","article-title":"The possible role of hemorheology in atherothrombogenesis","volume":"94","author":"Koenig","year":"1992","journal-title":"Atherosclerosis"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"26150","DOI":"10.1016\/S0021-9258(18)35729-6","article-title":"Lys-plasminogen is a significant intermediate in the activation of Glu-plasminogen during fibrinolysis in vitro","volume":"267","author":"Fredenburgh","year":"1992","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0014-2999(03)01528-0","article-title":"Inhibitory activity of kinetin on free radical formation of activated platelets in vitro and on thrombus formation in vivo","volume":"465","author":"Hsiao","year":"2003","journal-title":"Eur. J. Pharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1111\/j.1538-7836.2007.02461.x","article-title":"Platelet activation in acute pulmonary embolism","volume":"5","author":"Chung","year":"2007","journal-title":"J. Thromb. Haemost."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/B978-0-12-801401-1.00004-9","article-title":"Metabolomics in dyslipidemia","volume":"66","author":"Chen","year":"2014","journal-title":"Adv. Clin. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1021\/pr400872j","article-title":"Metabolomics reveals metabolite changes in acute pulmonary embolism","volume":"13","author":"Bujak","year":"2014","journal-title":"J. Proteome Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1111\/jth.13937","article-title":"Metabolomic analysis of 92 pulmonary embolism patients from a nested case-control study identifies metabolites associated with adverse clinical outcomes","volume":"16","author":"Zeleznik","year":"2017","journal-title":"J. Thromb. Haemost."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1021\/acsinfecdis.6b00124","article-title":"Tetrahydrobiopterin supplementation improves phenylalanine metabolism in a murine model of severe malaria","volume":"2","author":"Alkaitis","year":"2016","journal-title":"ACS Infect. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"19238","DOI":"10.1039\/C8RA00978C","article-title":"High-throughput metabolomics used to identify potential therapeutic targets of Guizhi Fuling Wan against endometriosis of cold coagulation and blood stasis","volume":"8","author":"Wu","year":"2018","journal-title":"RSC Adv."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1097\/01.fjc.0000154372.03531.e1","article-title":"Trans regulation of the \u03b12-adrenergic signal transduction pathway by chroni-blockade","volume":"45","author":"Schwencke","year":"2005","journal-title":"J. Cardiovasc. Pharm."},{"key":"ref_45","first-page":"80","article-title":"The role of histidine in metabolism","volume":"2","author":"Li","year":"1985","journal-title":"Prog. Physiol. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1002\/pmic.200500234","article-title":"Rapid protein identification using monolithic enzymatic microreactor and LC-ESI-MS\/MS","volume":"6","author":"Duan","year":"2006","journal-title":"Proteomics"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.3109\/13880209.2013.790065","article-title":"Study on hemostatic activities of the rhizome of Paris bashanensis","volume":"51","author":"Wang","year":"2013","journal-title":"Pharm. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1007\/s00109-011-0800-0","article-title":"A novel role of andrographolide, an NF-kappa B inhibitor on inhibition of platelet activation: The pivotal mechanisms of endothelial nitric oxide synthase\/cyclic GMP","volume":"89","author":"Lu","year":"2011","journal-title":"J. Mol. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.thromres.2006.10.012","article-title":"Gender and inter-species influence on coagulation tests of rats and mice","volume":"120","author":"Lemini","year":"2007","journal-title":"Thromb. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.jep.2016.04.016","article-title":"A new anti-fibrinolytic hemostatic compound 8-O-acetyl shanzhiside methylester extracted from Lamiophlomis rotata","volume":"187","author":"Fan","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.3390\/molecules16021378","article-title":"Effects of rutin and hesperidin and their Al (III) and Cu (II) complexes on in vitro plasma coagulation assays","volume":"16","author":"Vesna","year":"2011","journal-title":"Molecules"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1046\/j.1365-2141.2000.01953.x","article-title":"Antithrombotic effect of rutaecarpine, an alkaloid isolated from Evodia rutaecarpa on platelet plug formation in in vivo experiments","volume":"110","author":"Sheu","year":"2000","journal-title":"British J. Haematol."},{"key":"ref_53","first-page":"170","article-title":"Purification, biochemical properties and antithrombotic effect of a novel Streptomyces enzyme on carrageenan-induced mice tail thrombosis model","volume":"129","author":"Simkhada","year":"2012","journal-title":"J. Thromb. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/s11239-009-0341-3","article-title":"Factor Xa-like and fibrin (ogen)olytic activities of a serine protease from Hippasa agelenoidesspider venom gland extract","volume":"29","author":"Devaraja","year":"2010","journal-title":"J. Thromb. Thrombolys"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.ijbiomac.2015.04.075","article-title":"Antiplatelet and antithrombotic activity of a fibrin (ogen)olytic protease from Bacillus cereus strain FF01","volume":"79","author":"Majumdar","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.foodchem.2017.07.106","article-title":"A comparative UPLC-Q\/TOF-MS-based metabolomics approach for distinguishing Zingiber officinale Roscoe of two geographical origins","volume":"240","author":"Mais","year":"2017","journal-title":"Food Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/17\/4088\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:12:48Z","timestamp":1760188368000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/17\/4088"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,21]]},"references-count":56,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2019,9]]}},"alternative-id":["ijms20174088"],"URL":"https:\/\/doi.org\/10.3390\/ijms20174088","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,21]]}}}