{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:13:29Z","timestamp":1789913609479,"version":"4.0.1"},"reference-count":41,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T00:00:00Z","timestamp":1566950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundaci\u00f3n S\u00e9neca, Regi\u00f3n de Murcia (Spain)","award":["J\u00f3venes L\u00edderes en Investigaci\u00f3n 20646\/JLI\/18"],"award-info":[{"award-number":["J\u00f3venes L\u00edderes en Investigaci\u00f3n 20646\/JLI\/18"]}]},{"name":"Spanish Ministry of Economy and Competitiveness","award":["CTQ2017-87974-R"],"award-info":[{"award-number":["CTQ2017-87974-R"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Food peptides contain a very wide range of diversified structures, which explains their diverse range of functional activities. Proatherogenic endothelium is related to vasoconstriction, inflammation, and oxidative stress. In this line, four synthetic bioactive peptides from dry-cured pork ham, previously identified according to their Angiotensin I Converting Enzyme (ACE) inhibitory capacity and high bioavailability, were tested. Among them, KPVAAP displayed an estimated IC50 of 59.22 \u00b5M for human ACE inhibition, and docking simulations demonstrated the consistency of the noncompetitive binding with the protein. The addition of synthetic peptides to human endothelial cells significantly prevents the expression of genes related to endothelial dysfunction and inflammation (eNOS, ICAM-1, VCAM-1, IL-6) and lowers NF-\u03baB activation (all p &lt; 0.05). In silico dockings showed that the four bioactive peptides interact with the regulatory subunit NEMO of the NF-\u03baB transcription factor at the same site as other characterized inhibitors (CC2-LZ region). This is the first study linking experimental and computational approaches that shows NF-\u03baB to be the target of biopeptides of food origin. These multifunctional peptides from dry-cured pork ham make them good candidates for further research into their therapeutic or preventive use to attenuate the inflammatory atherosclerotic process.<\/jats:p>","DOI":"10.3390\/ijms20174204","type":"journal-article","created":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T11:23:18Z","timestamp":1566991398000},"page":"4204","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Multifunctional Peptides from Spanish Dry-Cured Pork Ham: Endothelial Responses and Molecular Modeling Studies"],"prefix":"10.3390","volume":"20","author":[{"given":"Sara Mar\u00eda","family":"Mart\u00ednez-S\u00e1nchez","sequence":"first","affiliation":[{"name":"Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, UCAM Universidad Cat\u00f3lica San Antonio de Murcia, Campus de los Jer\u00f3nimos s\/n, Guadalupe 30107, Murcia, Spain"},{"name":"Departamento Tecnolog\u00eda de la Alimentaci\u00f3n y Nutrici\u00f3n, UCAM Universidad Cat\u00f3lica San Antonio de Murcia, Campus de los Jer\u00f3nimos s\/n, Guadalupe 30107, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4468-7898","authenticated-orcid":false,"given":"Horacio","family":"P\u00e9rez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Bioinformatics and High Performance Computing Research Group (BIO-HPC), Computer Engineering Department, Universidad Cat\u00f3lica de Murcia (UCAM), Guadalupe 30107, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6649-2920","authenticated-orcid":false,"given":"Jos\u00e9","family":"Antonio Gabald\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento Tecnolog\u00eda de la Alimentaci\u00f3n y Nutrici\u00f3n, UCAM Universidad Cat\u00f3lica San Antonio de Murcia, Campus de los Jer\u00f3nimos s\/n, Guadalupe 30107, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Abell\u00e1n-Alem\u00e1n","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Riesgo Cardiovascular, UCAM Universidad Cat\u00f3lica San Antonio de Murcia, Campus de los Jer\u00f3nimos s\/n, Guadalupe 30107, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1948-1022","authenticated-orcid":false,"given":"Silvia","family":"Montoro-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, UCAM Universidad Cat\u00f3lica San Antonio de Murcia, Campus de los Jer\u00f3nimos s\/n, Guadalupe 30107, Murcia, Spain"},{"name":"C\u00e1tedra de Riesgo Cardiovascular, UCAM Universidad Cat\u00f3lica San Antonio de Murcia, Campus de los Jer\u00f3nimos s\/n, Guadalupe 30107, Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/apha.12646","article-title":"Endothelial dysfunction and vascular disease\u2014A 30th anniversary update","volume":"219","author":"Vanhoutte","year":"2017","journal-title":"Acta Physiol. 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