{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T20:12:16Z","timestamp":1770754336948,"version":"3.50.0"},"reference-count":33,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T00:00:00Z","timestamp":1570579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009887","name":"Regione Siciliana","doi-asserted-by":"publisher","award":["CheMISt-CUPG77B17000110001"],"award-info":[{"award-number":["CheMISt-CUPG77B17000110001"]}],"id":[{"id":"10.13039\/501100009887","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>NLRP3 (NOD-like receptor family, pyrin domain-containing protein 3) activation has been linked to several chronic pathologies, including atherosclerosis, type-II diabetes, fibrosis, rheumatoid arthritis, and Alzheimer\u2019s disease. Therefore, NLRP3 represents an appealing target for the development of innovative therapeutic approaches. A few companies are currently working on the discovery of selective modulators of NLRP3 inflammasome. Unfortunately, limited structural data are available for this target. To date, MCC950 represents one of the most promising noncovalent NLRP3 inhibitors. Recently, a possible region for the binding of MCC950 to the NLRP3 protein was described but no details were disclosed regarding the key interactions. In this communication, we present an in silico multiple approach as an insight useful for the design of novel NLRP3 inhibitors. In detail, combining different computational techniques, we propose consensus-retrieved protein residues that seem to be essential for the binding process and for the stabilization of the protein\u2013ligand complex.<\/jats:p>","DOI":"10.3390\/ijms20204974","type":"journal-article","created":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T03:14:33Z","timestamp":1570590873000},"page":"4974","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["In Silico Insights towards the Identification of NLRP3 Druggable Hot Spots"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3490-1179","authenticated-orcid":false,"given":"Nedra","family":"Mekni","sequence":"first","affiliation":[{"name":"Drug Discovery Unit, Fondazione Ri.MED, 90133 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4087-2554","authenticated-orcid":false,"given":"Maria","family":"De Rosa","sequence":"additional","affiliation":[{"name":"Drug Discovery Unit, Fondazione Ri.MED, 90133 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0712-8440","authenticated-orcid":false,"given":"Chiara","family":"Cipollina","sequence":"additional","affiliation":[{"name":"Drug Discovery Unit, Fondazione Ri.MED, 90133 Palermo, Italy"},{"name":"Istituto per la Ricerca e l\u2019Innovazione Biomedica (IRIB)-Consiglio Nazionale delle Ricerche, 90146 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5032-4888","authenticated-orcid":false,"given":"Maria Rita","family":"Gulotta","sequence":"additional","affiliation":[{"name":"Drug Discovery Unit, Fondazione Ri.MED, 90133 Palermo, Italy"},{"name":"Dipartimento STEBICEF, Universit\u00e0 degli Studi di Palermo, 90100 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0917-2226","authenticated-orcid":false,"given":"Giada","family":"De Simone","sequence":"additional","affiliation":[{"name":"Drug Discovery Unit, Fondazione Ri.MED, 90133 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8986-307X","authenticated-orcid":false,"given":"Jessica","family":"Lombino","sequence":"additional","affiliation":[{"name":"Drug Discovery Unit, Fondazione Ri.MED, 90133 Palermo, Italy"},{"name":"Dipartimento STEBICEF, Universit\u00e0 degli Studi di Palermo, 90100 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Padova","sequence":"additional","affiliation":[{"name":"Drug Discovery Unit, Fondazione Ri.MED, 90133 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2181-2468","authenticated-orcid":false,"given":"Ugo","family":"Perricone","sequence":"additional","affiliation":[{"name":"Drug Discovery Unit, Fondazione Ri.MED, 90133 Palermo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/nrd.2018.97","article-title":"Targeting the NLRP3 Inflammasome in Inflammatory Diseases","volume":"17","author":"Mangan","year":"2018","journal-title":"Nat. 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