{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:34:46Z","timestamp":1790418886618,"version":"4.1.0"},"reference-count":60,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2019,10,15]],"date-time":"2019-10-15T00:00:00Z","timestamp":1571097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PO FESR Sicilia 2007\u20132013","award":["CUP G73F11000050004"],"award-info":[{"award-number":["CUP G73F11000050004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Background: Obesity is a pathological condition that has reached epidemic proportions; hence, it is necessary to find novel strategies aimed at fighting this disease. The present study was designed to evaluate the effect of a flavonoid-rich extract of orange (Citrus sinensis) juice (OJe) in diet-induced obese zebrafish. Methods: Adult zebrafish were divided into four diet groups: (i) normally fed (NF); (ii) overfed (OF); (iii) NF supplemented with OJe (5 mL\/L in fish water; NF + OJe); and (iv) OF supplemented with OJe (OF + OJe). Each week, body weight (BW) and body mass index (BMI) were measured, and, at the end of the fifth week, euthanized zebrafish were processed for both microscopic evaluations and qPCR analyses. Results: In OF zebrafish, OJe significantly decreased both BW and BMI values and lowered the visceral adipose tissue, while it had little effect in the NF group. Moreover, it significantly reduced adipocyte cell size in both NF and OF groups in both visceral and subcutaneous adipose tissues, as well as their number in OF fish. Finally, OJe modulated some obesity-related genes, such as leptin A, ghrelin, orexin, pro-opiomelanocortin (POMC), and neuropeptide Y (NPY), in both gut and brain. Conclusion: This study adds new insights into the anti-obesity properties of orange juice and its flavonoids, suggesting their role as weight management agents through a lipolytic action linked to a restoration of metabolism-regulating gene expression.<\/jats:p>","DOI":"10.3390\/ijms20205116","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T03:32:54Z","timestamp":1571196774000},"page":"5116","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Effects of a Flavonoid-Rich Extract from Citrus sinensis Juice on a Diet-Induced Obese Zebrafish"],"prefix":"10.3390","volume":"20","author":[{"given":"Giuseppe","family":"Montalbano","sequence":"first","affiliation":[{"name":"Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5144-8719","authenticated-orcid":false,"given":"Manuela","family":"Mania","sequence":"additional","affiliation":[{"name":"Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Guerrera","sequence":"additional","affiliation":[{"name":"Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaria","family":"Laur\u00e0","sequence":"additional","affiliation":[{"name":"Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Abbate","sequence":"additional","affiliation":[{"name":"Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Levanti","sequence":"additional","affiliation":[{"name":"Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8670-3123","authenticated-orcid":false,"given":"Alessandro","family":"Maugeri","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonino","family":"German\u00e0","sequence":"additional","affiliation":[{"name":"Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6492-7820","authenticated-orcid":false,"given":"Michele","family":"Navarra","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17034","DOI":"10.1038\/nrdp.2017.34","article-title":"Obesity","volume":"3","author":"Hu","year":"2017","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_2","unstructured":"World Health Organization (2018, February 16). World Health Organization Obesity and Overweight Fact Sheet. Available online: http:\/\/www.who.int\/mediacentre\/factsheets\/fs311\/en\/."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1242\/dmm.011346","article-title":"The emerging use of zebrafish to model metabolic disease","volume":"6","author":"Seth","year":"2013","journal-title":"Dis. Model. Mech."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.bbr.2013.08.012","article-title":"Developing \u2018integrative\u2019 zebrafish models of behavioral and metabolic disorders","volume":"256","author":"Nguyen","year":"2013","journal-title":"Behav. Brain Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Oka, T., Nishimura, Y., Zang, L., Hirano, M., Shimada, Y., Wang, Z., Umemoto, N., Kuroyanagi, J., Nishimura, N., and Tanaka, T. (2010). Diet-induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. BMC Physiol., 10.","DOI":"10.1186\/1472-6793-10-21"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1038\/nrd4627","article-title":"Zebrafish as tools for drug discovery","volume":"14","author":"MacRae","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1042\/BJ20100293","article-title":"Zebrafish: Gaining popularity in lipid research","volume":"429","author":"Salo","year":"2010","journal-title":"Biochem. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.aanat.2017.03.005","article-title":"Expression and distribution of leptin and its receptors in the digestive tract of DIO (diet-induced obese) zebrafish","volume":"212","author":"Mania","year":"2017","journal-title":"Ann. Anat."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.aanat.2015.11.003","article-title":"Morphological differences in adipose tissue and changes in BDNF\/Trkb expression in brain and gut of a diet induced obese zebrafish model","volume":"204","author":"Montalbano","year":"2016","journal-title":"Ann. Anat."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1007\/s12020-018-1653-x","article-title":"Melatonin treatment suppresses appetite genes and improves adipose tissue plasticity in diet-induced obese zebrafish","volume":"62","author":"Montalbano","year":"2018","journal-title":"Endocrine"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1080\/10408398.2011.625574","article-title":"Cranberry and recurrent cystitis: More than marketing?","volume":"54","author":"Micali","year":"2014","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.phanu.2015.03.002","article-title":"Role of natural antioxidants and potential use of bergamot in treating rheumatoid arthritis","volume":"3","author":"Marino","year":"2015","journal-title":"PharmaNutrition"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cirmi, S., Ferlazzo, N., Lombardo, G.E., Maugeri, A., Calapai, G., Gangemi, S., and Navarra, M. (2016). Chemopreventive Agents and Inhibitors of Cancer Hallmarks: May Citrus Offer New Perspectives?. Nutrients, 8.","DOI":"10.3390\/nu8110698"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Cirmi, S., Ferlazzo, N., Lombardo, G.E., Ventura-Spagnolo, E., Gangemi, S., Calapai, G., and Navarra, M. (2016). Neurodegenerative Diseases: Might Citrus Flavonoids Play a Protective Role?. Molecules, 21.","DOI":"10.3390\/molecules21101312"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1459497","DOI":"10.1155\/2017\/1459497","article-title":"The Beneficial Effects of Quercetin, Curcumin, and Resveratrol in Obesity","volume":"2017","author":"Zhao","year":"2017","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Sun, N.N., Wu, T.Y., and Chau, C.F. (2016). Natural Dietary and Herbal Products in Anti-Obesity Treatment. Molecules, 21.","DOI":"10.3390\/molecules21101351"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1179\/1476830513Y.0000000099","article-title":"A natural solution for obesity: Bioactives for the prevention and treatment of weight gain. A review","volume":"18","author":"Schellekens","year":"2015","journal-title":"Nutr. Neurosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1039\/C7FO02066J","article-title":"Citrus peel extracts attenuated obesity and modulated gut microbiota in mice with high-fat diet-induced obesity","volume":"9","author":"Tung","year":"2018","journal-title":"Food Funct."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1146\/annurev-nutr-071715-050718","article-title":"Citrus Flavonoids as Regulators of Lipoprotein Metabolism and Atherosclerosis","volume":"36","author":"Mulvihill","year":"2016","journal-title":"Annu. Rev. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kawser Hossain, M., Abdal Dayem, A., Han, J., Yin, Y., Kim, K., Kumar Saha, S., Yang, G.M., Choi, H.Y., and Cho, S.G. (2016). Molecular Mechanisms of the Anti-Obesity and Anti-Diabetic Properties of Flavonoids. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17040569"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Moseti, D., Regassa, A., and Kim, W.K. (2016). Molecular Regulation of Adipogenesis and Potential Anti-Adipogenic Bioactive Molecules. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17010124"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1080\/10408398.2013.862611","article-title":"100% Citrus juice: Nutritional contribution, dietary benefits, and association with anthropometric measures","volume":"57","author":"Rampersaud","year":"2017","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/1475-2891-11-107","article-title":"100% orange juice consumption is associated with better diet quality, improved nutrient adequacy, decreased risk for obesity, and improved biomarkers of health in adults: National Health and Nutrition Examination Survey, 2003\u20132006","volume":"11","author":"Nicklas","year":"2012","journal-title":"Nutr. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3624094","DOI":"10.1155\/2018\/3624094","article-title":"Clinical Pharmacology of Citrus aurantium and Citrus sinensis for the Treatment of Anxiety","volume":"2018","author":"Mannucci","year":"2018","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"420","DOI":"10.3389\/fphar.2017.00420","article-title":"Anticancer Potential of Citrus Juices and Their Extracts: A Systematic Review of Both Preclinical and Clinical Studies","volume":"8","author":"Cirmi","year":"2017","journal-title":"Front. Pharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.3945\/ajcn.111.031088","article-title":"Effects of red orange juice intake on endothelial function and inflammatory markers in adult subjects with increased cardiovascular risk","volume":"95","author":"Buscemi","year":"2012","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1038\/ijo.2009.266","article-title":"Blood orange juice inhibits fat accumulation in mice","volume":"34","author":"Titta","year":"2010","journal-title":"Int. J. Obes."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.3945\/jn.115.213660","article-title":"Normal or High Polyphenol Concentration in Orange Juice Affects Antioxidant Activity, Blood Pressure, and Body Weight in Obese or Overweight Adults","volume":"145","author":"Aguilera","year":"2015","journal-title":"J. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.1080\/14786419.2014.1000897","article-title":"Clinical evaluation of Moro (Citrus sinensis (L.) Osbeck) orange juice supplementation for the weight management","volume":"29","author":"Cardile","year":"2015","journal-title":"Nat. Prod. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1672567","DOI":"10.1155\/2017\/1672567","article-title":"Effect of Red Orange Juice Consumption on Body Composition and Nutritional Status in Overweight\/Obese Female: A Pilot Study","volume":"2017","author":"Azzini","year":"2017","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.nut.2016.12.020","article-title":"Orange juice allied to a reduced-calorie diet results in weight loss and ameliorates obesity-related biomarkers: A randomized controlled trial","volume":"38","author":"Ribeiro","year":"2017","journal-title":"Nutrition"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"354","DOI":"10.3389\/fnins.2017.00354","article-title":"Hypothalamic Integration of Metabolic, Endocrine, and Circadian Signals in Fish: Involvement in the Control of Food Intake","volume":"11","author":"Delgado","year":"2017","journal-title":"Front. Neurosci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/7854_2016_51","article-title":"Roles for Orexin\/Hypocretin in the Control of Energy Balance and Metabolism","volume":"33","author":"Goforth","year":"2017","journal-title":"Curr. Top. Behav. Neurosci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/j.lwt.2014.02.060","article-title":"Citrus bioactive phenolics: Role in the obesity treatment","volume":"59","author":"Nakajima","year":"2014","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"957031","DOI":"10.1155\/2015\/957031","article-title":"Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress","volume":"2015","author":"Ferlazzo","year":"2015","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.etap.2016.03.005","article-title":"Natural iron chelators: Protective role in A549 cells of flavonoids-rich extracts of Citrus juices in Fe(3+)-induced oxidative stress","volume":"43","author":"Ferlazzo","year":"2016","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.jff.2016.12.038","article-title":"A flavonoid-rich extract of orange juice reduced oxidative stress in an experimental model of inflammatory bowel disease","volume":"30","author":"Fusco","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Citraro, R., Navarra, M., Leo, A., Donato Di Paola, E., Santangelo, E., Lippiello, P., Aiello, R., Russo, E., and De Sarro, G. (2016). The Anticonvulsant Activity of a Flavonoid-Rich Extract from Orange Juice Involves both NMDA and GABA-Benzodiazepine Receptor Complexes. Molecules, 21.","DOI":"10.3390\/molecules21091261"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1186\/s12967-017-1343-0","article-title":"Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes","volume":"15","author":"Lasa","year":"2017","journal-title":"J. Transl. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3576","DOI":"10.1039\/C7FO00679A","article-title":"Screening of potential anti-adipogenic effects of phenolic compounds showing different chemical structure in 3T3-L1 preadipocytes","volume":"8","author":"Lasa","year":"2017","journal-title":"Food Funct."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.jnutbio.2012.03.014","article-title":"Nobiletin improves obesity and insulin resistance in high-fat diet-induced obese mice","volume":"24","author":"Lee","year":"2013","journal-title":"J. Nutr. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nature06902","article-title":"Dynamics of fat cell turnover in humans","volume":"453","author":"Spalding","year":"2008","journal-title":"Nature"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s00394-018-1663-8","article-title":"Differential effects of dietary flavonoids on adipogenesis","volume":"58","author":"Khalilpourfarshbafi","year":"2019","journal-title":"Eur. J. Nutr."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1152\/physrev.00031.2014","article-title":"Ghrelin, CCK, GLP-1, and PYY (3\u201336): Secretory Controls and Physiological Roles in Eating and Glycemia in Health, Obesity, and After RYGB","volume":"97","author":"Steinert","year":"2017","journal-title":"Physiol. Rev."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1021\/acs.jafc.7b05104","article-title":"Natural Dietary Products and Their Effects on Appetite Control","volume":"66","author":"Suh","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/nrendo.2016.222","article-title":"The cellular and molecular bases of leptin and ghrelin resistance in obesity","volume":"13","author":"Cui","year":"2017","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"186","DOI":"10.14310\/horm.2002.1672","article-title":"Effects of ghrelin in energy balance and body weight homeostasis","volume":"15","author":"Mihalache","year":"2016","journal-title":"Hormones"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"60","DOI":"10.2174\/2212798406666140825120623","article-title":"Hesperidin potentiates ghrelin signaling","volume":"6","author":"Suzuki","year":"2014","journal-title":"Recent Pat. Food Nutr. Agric."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/B978-0-12-394623-2.00006-8","article-title":"Energy expenditure: Role of orexin","volume":"89","author":"Teske","year":"2012","journal-title":"Vitam. Horm."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.yhbeh.2014.06.004","article-title":"Orexin A enhances locomotor activity and induces anxiogenic-like action in the goldfish, Carassius auratus","volume":"66","author":"Nakamachi","year":"2014","journal-title":"Horm. Behav."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/ijo.2017.276","article-title":"Neuromodulation of orexin neurons reduces diet-induced adiposity","volume":"42","author":"Zink","year":"2018","journal-title":"Int. J. Obes."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1210\/en.2006-1389","article-title":"Role of the central melanocortin circuitry in adaptive thermogenesis of brown adipose tissue","volume":"148","author":"Murphy","year":"2007","journal-title":"Endocrinology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1172\/JCI92035","article-title":"Inflammatory mechanisms linking obesity and metabolic disease","volume":"127","author":"Saltiel","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ferlazzo, N., Cirmi, S., Calapai, G., Ventura-Spagnolo, E., Gangemi, S., and Navarra, M. (2016). Anti-Inflammatory Activity of Citrus bergamia Derivatives: Where Do We Stand?. Molecules, 21.","DOI":"10.3390\/molecules21101273"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Musumeci, L., Maugeri, A., Cirmi, S., Lombardo, G.E., Russo, C., Gangemi, S., Calapai, G., and Navarra, M. (2019). Citrus fruits and their flavonoids in inflammatory bowel disease: An overview. Nat. Prod. Res., 1\u201315.","DOI":"10.1080\/14786419.2019.1601196"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Maugeri, A., Cirmi, S., Minciullo, P.L., Gangemi, S., Calapai, G., Mollace, V., and Navarra, M. (2019). Citrus fruits and inflammaging: A systematic review. Phytochem. Rev.","DOI":"10.1007\/s11101-019-09613-3"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"203","DOI":"10.3389\/fphar.2016.00203","article-title":"Anti-inflammatory and Antioxidant Effects of Flavonoid-Rich Fraction of Bergamot Juice (BJe) in a Mouse Model of Intestinal Ischemia\/Reperfusion Injury","volume":"7","author":"Impellizzeri","year":"2016","journal-title":"Front. Pharmacol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1080\/01635581.2015.1073760","article-title":"Flavonoid Fraction of Citrus reticulata Juice Reduces Proliferation and Migration of Anaplastic Thyroid Carcinoma Cells","volume":"67","author":"Celano","year":"2015","journal-title":"Nutr. Cancer"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Navarra, M., Femia, A.P., Romagnoli, A., Tortora, K., Luceri, C., Cirmi, S., Ferlazzo, N., and Caderni, G. (2019). A flavonoid-rich extract from bergamot juice prevents carcinogenesis in a genetic model of colorectal cancer, the Pirc rat (F344\/NTac-Apc(am1137)). Eur. J. Nutr.","DOI":"10.1007\/s00394-019-01948-z"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"RC31","DOI":"10.1007\/BF03349220","article-title":"Growth inhibition of medullary thyroid carcinoma cells by pyrazolo-pyrimidine derivates","volume":"30","author":"Morisi","year":"2007","journal-title":"J. Endocrinol. Investig."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/20\/5116\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:26:38Z","timestamp":1760189198000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/20\/5116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,15]]},"references-count":60,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2019,10]]}},"alternative-id":["ijms20205116"],"URL":"https:\/\/doi.org\/10.3390\/ijms20205116","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,15]]}}}