{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:30:18Z","timestamp":1790800218518,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T00:00:00Z","timestamp":1572307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science &amp; Technology of Taiwan, R.O.C","award":["107-2221-E-155 -021 -"],"award-info":[{"award-number":["107-2221-E-155 -021 -"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Graphene oxide (GO) composites with various metal nanoparticles (NPs) are attracting increasing interest owing to their broad scope in biomedical applications. Here, microwave-assisted chemical reduction was used to deposit nano-silver and zinc oxide NPs (Ag and ZnO NPs) on the surface of reduced GO (rGO) at the following weight percentages: 5.34% Ag\/rGO, 7.49% Ag\/rGO, 6.85% ZnO\/rGO, 16.45% ZnO\/rGO, 3.47\/34.91% Ag\/ZnO\/rGO, and 7.08\/15.28% Ag\/ZnO\/rGO. These materials were tested for antibacterial activity, and 3.47\/34.91% Ag\/ZnO\/rGO and 7.08\/15.28% Ag\/ZnO\/rGO exhibited better antibacterial activity than the other tested materials against the gram-negative bacterium Escherichia coli K12. At 1000 ppm, both these Ag\/ZnO\/rGO composites had better killing properties against both E. coli K12 and the gram-positive bacterium Staphylococcus aureus SA113 than Ag\/rGO and ZnO\/rGO did. RedoxSensor flow cytometry showed that 3.47\/34.91% Ag\/ZnO\/rGO and 7.08\/15.28% Ag\/ZnO\/rGO decreased reductase activity and affected membrane integrity in the bacteria. At 100 ppm, these two composites affected membrane integrity more in E. coli, while 7.08\/15.28% Ag\/ZnO\/rGO considerably decreased reductase activity in S. aureus. Thus, the 3.47\/34.91% and 7.08%\/15.28% Ag\/ZnO\/rGO nanocomposites can be applied not only as antibacterial agents but also in a variety of biomedical materials such as sensors, photothermal therapy, drug delivery, and catalysis, in the future.<\/jats:p>","DOI":"10.3390\/ijms20215394","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T05:18:26Z","timestamp":1572499106000},"page":"5394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Antibacterial Property of Composites of Reduced Graphene Oxide with Nano-Silver and Zinc Oxide Nanoparticles Synthesized Using a Microwave-Assisted Approach"],"prefix":"10.3390","volume":"20","author":[{"given":"Yi-Huang","family":"Hsueh","sequence":"first","affiliation":[{"name":"Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Te","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Ting","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping-Han","family":"Tsai","sequence":"additional","affiliation":[{"name":"Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 32003, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City 220, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Ju","family":"Ke","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Sciences, and Research Center for Bacterial Pathogenesis, Chang Gung University, Taoyuan 33302, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1016\/j.carbon.2011.12.014","article-title":"Amorphous structural models for graphene oxides","volume":"50","author":"Liu","year":"2012","journal-title":"Carbon"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.compositesa.2016.04.011","article-title":"Review of functionalization, structure and properties of graphene\/polymer composite fibers","volume":"87","author":"Ji","year":"2016","journal-title":"Compos. 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