{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:15:43Z","timestamp":1790748943025,"version":"4.1.0"},"reference-count":11,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Background: Laboratory of allergic diseases 2 (LAD2) human mast cells were developed over 15 years ago and have been distributed worldwide for studying mast cell proliferation, receptor expression, mediator release\/inhibition, and signaling. LAD2 cells were derived from CD34+ cells following marrow aspiration of a patient with aggressive mastocytosis with no identified mutations in KIT. Another aspiration gave rise to a second cell line which has recently been re-established (LADR). We queried whether LADR had unique properties for the preclinical study of human mast cell biology. Methods: LADR and LAD2 cells were cultured under identical conditions. Experiments examined proliferation, beta-hexosaminidase (\u03b2-hex) release, surface receptor and granular protease expression, infectivity with HIV, and gene expression. Results: LADR cells were larger and more granulated as seen with Wright\u2013Giemsa staining and flow cytometry, with cell numbers doubling in 4 weeks, in contrast to LAD2 cells, which doubled every 2 weeks. Both LADR and LAD2 cells released granular contents following aggregation of Fc\u03b5RI. LADR cells showed log-fold increases in Fc\u03b5RI\/CD117 and expressed CD13, CD33, CD34, CD63, CD117, CD123, CD133, CD184, CD193, and CD195, while LAD2 cells expressed CD33, CD34, CD63, CD117, CD133, CD193 but not CD13, CD123, CD184, or CD195. LADR tryptase expression was one-log-fold increased. LADR cell and LAD2 cell chymase expression were similar. Both cell lines could be infected with T-tropic, M-tropic, and dual tropic HIV. Following monomeric human IgE stimulation, LADR cells showed greater surface receptor and mRNA expression for CD184 and CD195. Expression arrays revealed differences in gene upregulation, especially for the suppressor of cytokine signaling (SOCS) family of genes with their role in JAK2\/STAT3 signaling and cellular myelocytomatosis oncogene (c-MYC) in cell growth and regulation. Conclusions: LADR cells are thus unique in that they exhibit a slower proliferation rate, are more advanced in development, have increased Fc\u03b5RI\/CD117 and tryptase expression, have a different profile of gene expression, and show earlier infectivity with HIV-BAL, LAV, and TYBE when compared to LAD2 cells. This new cell line is thus a valuable addition to the few Fc\u03b5RI+ human mast cell lines previously described and available for scientific inquiry.<\/jats:p>","DOI":"10.3390\/ijms20225520","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T02:48:31Z","timestamp":1573094911000},"page":"5520","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Description and Characterization of a Novel Human Mast Cell Line for Scientific Study"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7668-0247","authenticated-orcid":false,"given":"Arnold","family":"S. Kirshenbaum","sequence":"first","affiliation":[{"name":"Mast Cell Biology Section, Laboratory of Allergic Diseases, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhi","family":"Yin","sequence":"additional","affiliation":[{"name":"Mast Cell Biology Section, Laboratory of Allergic Diseases, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Bruce","family":"Sundstrom","sequence":"additional","affiliation":[{"name":"AIDS Research Review Branch\/SRP, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geethani","family":"Bandara","sequence":"additional","affiliation":[{"name":"Mast Cell Biology Section, Laboratory of Allergic Diseases, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7781-8260","authenticated-orcid":false,"given":"Dean","family":"D. Metcalfe","sequence":"additional","affiliation":[{"name":"Mast Cell Biology Section, Laboratory of Allergic Diseases, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/0145-2126(88)90050-1","article-title":"Establishment of an immature mast cell line from a patient with mast cell leukemia","volume":"12","author":"Butterfield","year":"1988","journal-title":"Leukemia Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/S0145-2126(02)00343-0","article-title":"Characterization of novel stem cell factor responsive human mast cell lines LAD 1 and 2 established from a patient with mast cell sarcoma\/leukemia; activation following aggregation of FceRI or FceRI","volume":"27","author":"Kirshenbaum","year":"2003","journal-title":"Leukemia Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/j.jaci.2010.12.1101","article-title":"Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional Fc\u03b5RI","volume":"127","author":"Laidlaw","year":"2011","journal-title":"J. Allergy Clin. Immun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1182\/blood-2013-10-534685","article-title":"A new human mast cell line expressing a functional IgE receptor converts to tumorigenic growth by KIT D816V transfection","volume":"124","author":"Saleh","year":"2014","journal-title":"Blood"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.3324\/haematol.2018.195867","article-title":"Preclinical human models and emerging therapeutics for advanced systemic mastocytosis","volume":"103","author":"Arock","year":"2018","journal-title":"Haematologica"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6401","DOI":"10.4049\/jimmunol.0801481","article-title":"IgE-FcepsilonRI interactions determine HIV coreceptor usage and susceptibility to infection during ontogeny of mast cells","volume":"182","author":"Sundstrom","year":"2009","journal-title":"J. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7.37.1","DOI":"10.1002\/0471142735.im0737s90","article-title":"Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood","volume":"90","author":"Radinger","year":"2010","journal-title":"Curr. Protoc. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7.38.1","DOI":"10.1002\/0471142735.im0738s91","article-title":"Measuring mast cell mediator release","volume":"91","author":"Kuehn","year":"2010","journal-title":"Curr. Protoc. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5428","DOI":"10.4049\/jimmunol.1103366","article-title":"Stem cell factor programs the mast cell activation phenotype","volume":"188","author":"Tomonobu","year":"2012","journal-title":"J. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1016\/j.exphem.2013.05.005","article-title":"KIT GNNK splice variants: Expression in systemic mastocytosis and influence on the activating potential of the D816V mutation in mast cells","volume":"41","author":"Eunice","year":"2013","journal-title":"Exp. Hematol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5293","DOI":"10.1182\/blood-2006-11-058438","article-title":"Human tissue mast cells are an inducible reservoir of persistent HIV infection","volume":"109","author":"Sundstrom","year":"2007","journal-title":"Blood"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/22\/5520\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:32:09Z","timestamp":1760189529000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/20\/22\/5520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,6]]},"references-count":11,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2019,11]]}},"alternative-id":["ijms20225520"],"URL":"https:\/\/doi.org\/10.3390\/ijms20225520","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,6]]}}}