{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T20:54:48Z","timestamp":1773694488896,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","award":["2017-0285"],"award-info":[{"award-number":["2017-0285"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Diabetes is associated with vascular complications, such as impaired wound healing and accelerated vascular growth. The different clinical manifestations, such as retinopathy and nephropathy, reveal the severity of enhanced vascular growth known as angiogenesis. This study was performed to evaluate the effects of an extract of Ishige okamurae (IO) and its constituent, Ishophloroglucin A (IPA) on high glucose-induced angiogenesis. A transgenic zebrafish (flk:EGFP) embryo model was used to evaluate vessel growth. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), gap closure, transwell, and Matrigel\u00ae assays were used to analyze the proliferation, migration, and capillary formation of EA.hy926 cells. Moreover, protein expression were determined using western blotting. IO extract and IPA suppressed vessel formation in the transgenic zebrafish (flk:EGFP) embryo. IPA attenuated cell proliferation, cell migration, and capillary-like structure formation in high glucose-treated human vascular endothelial cells. Further, IPA down regulated the expression of high glucose-induced vascular endothelial growth factor receptor 2 (VEGFR-2) and downstream signaling molecule cascade. Overall, the IO extract and IPA exhibited anti-angiogenic effects against high glucose-induced angiogenesis, suggesting their potential for use as therapeutic agents in diabetes-related angiogenesis.<\/jats:p>","DOI":"10.3390\/ijms20225542","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T06:52:36Z","timestamp":1573109556000},"page":"5542","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Ishige okamurae Extract and Its Constituent Ishophloroglucin A Attenuated In Vitro and In Vivo High Glucose-Induced Angiogenesis"],"prefix":"10.3390","volume":"20","author":[{"given":"K.H.N.","family":"Fernando","sequence":"first","affiliation":[{"name":"Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye-Won","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3299-7266","authenticated-orcid":false,"given":"You-Jin","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea"},{"name":"Marine Science Institute, Jeju National University, Jeju 63333, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5797-500X","authenticated-orcid":false,"given":"BoMi","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea"},{"name":"Marine Science Institute, Jeju National University, Jeju 63333, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.2174\/187152207779802536","article-title":"New Horizons in Diabetes Therapy: The Angiogenesis Paradox in Diabetes: Description of the Problem and Presentation of a Unifying Hypothesis","volume":"7","author":"Waltenberger","year":"2007","journal-title":"Immunol. 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