{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:17:28Z","timestamp":1791001048894,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T00:00:00Z","timestamp":1573776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Gout Party is a Chinese medicine prescription composed of Aconiti Lateralis Radix Praeparaia, Aconiti Radix Cocta, Cremastrae Pseudobulbus Pleiones Pseudobulbus, Smilacis Glabrae Rhizoma, Rehmanniae Radix, and Glycyrrhizae Radix et Rhizoma, which can relieve joint pain caused by gouty arthritis (GA) and rheumatoid, and has a therapeutic effect on acute gouty arthritis (AGA). However, little information is available on the molecular biological basis and therapeutic mechanism of Gout Party for the treatment of AGA. AGA model was established by injecting sodium urate, and colchicine served as a positive control drug. We established a metabolomic method based on ultra-high-performance liquid chromatography\u2013tandem quadrupole\/time-of-flight mass spectrometry (UHPLC\u2013Q\u2013TOF\/MS) to analyze the plasma samples of model group rats and blank group rats. Multiple statistical analyses, including principal component analysis (PCA) and partial least square discrimination analysis (PLS-DA), were used to examine metabolite profile changes in plasma samples. Finally, we identified 2\u2013ketobutyric acid, 3\u2013hexenedioic acid, but\u20132\u2013enoic acid, and so on; 22 endogenous metabolites associated with AGA. After successful molding, we found that 2\u2013ketobutyric acid, 3\u2013hexenedioic acid, but\u20132\u2013enoic acid, argininic acid, galactonic acid, lactic acid, equol 4\u2032\u2013O\u2013glucuronide, deoxycholic acid glycine conjugate, glycocholic acid, sphinganine 1\u2013phosphate, LPE (0:0\/20:3), LPE (0:0\/16:0), LPC (15:0) decreased significantly (p &lt; 0.05 or p &lt; 0.01), alanine, erythrulose, 3\u2013dehydrocarnitine, m\u2013methylhippuric acid, 3\u2013hydroxyoctanoic acid, p\u2013cresol sulfate, estriol 3\u2013sulfate 16\u2013glucuronide, 10\u2013hydroxy\u20139\u2013(phosphonooxy)octadecenoate, docosahexaenoic acid increased significantly (p &lt; 0.05 or p &lt; 0.01). After Gout Party treatment, 14 biomarkers had a tendency to normal conditions. These above biomarkers were mainly involved in fatty acid metabolism, bile acid metabolism, amino acid metabolism, and energy metabolism pathways. These results suggested that Gout Party exerted therapeutic effects of treating AGA by improving energy metabolism disorder and amino acid metabolism dysfunction, and attenuating fatty acid metabolism abnormal and inflammation. The results of this experiment provided a reference for revealing the metabolic mechanism produced by Gout Party in the treatment of AGA, but the subsequent studies need to be further improved and supported by relevant cell experiments and clinical experiments.<\/jats:p>","DOI":"10.3390\/ijms20225753","type":"journal-article","created":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T11:24:32Z","timestamp":1573817072000},"page":"5753","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Plasma Metabolic Profiling Analysis of Gout Party on Acute Gout Arthritis Rats Based on UHPLC\u2013Q\u2013TOF\/MS Combined with Multivariate Statistical Analysis"],"prefix":"10.3390","volume":"20","author":[{"given":"Yuming","family":"Wang","sequence":"first","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine, No. 10, Poyang Lake Road, West Zone, Tuanbo New City, Jinghai District, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenghao","family":"Bi","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine, No. 10, Poyang Lake Road, West Zone, Tuanbo New City, Jinghai District, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentao","family":"Pang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sports and Health, Tianjin Sport University, No. 16, Donghai Road, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuechen","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine, No. 10, Poyang Lake Road, West Zone, Tuanbo New City, Jinghai District, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine, No. 10, Poyang Lake Road, West Zone, Tuanbo New City, Jinghai District, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine, No. 10, Poyang Lake Road, West Zone, Tuanbo New City, Jinghai District, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianpu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine, No. 10, Poyang Lake Road, West Zone, Tuanbo New City, Jinghai District, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yungang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sports and Health, Tianjin Sport University, No. 16, Donghai Road, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0455-0969","authenticated-orcid":false,"given":"Yubo","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine, No. 10, Poyang Lake Road, West Zone, Tuanbo New City, Jinghai District, Tianjin 300193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"ITC1","DOI":"10.7326\/AITC201607050","article-title":"Clinical practice. 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