{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T20:46:28Z","timestamp":1768077988187,"version":"3.49.0"},"reference-count":43,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2019,11,16]],"date-time":"2019-11-16T00:00:00Z","timestamp":1573862400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","award":["1107-569-33704"],"award-info":[{"award-number":["1107-569-33704"]}]},{"name":"Universidad de Cartagena","award":["project Acta 013-2017"],"award-info":[{"award-number":["project Acta 013-2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The presence of hemoglobin A-S (HbAS) in erythrocytes has been related to the high production of reactive oxygen species (ROS) and an increased in intracellular oxidative stress that affects the progress of Plasmodium erythrocytic cycle life and attenuates its serious clinical symptoms. Nevertheless, oxidative effects on P. falciparum proteome across the intraerythrocytic cycle in the presence of HbAS traits have not been described yet. Here, an immune dot-blot assay was used to quantify the carbonyl index (C.I) on P. falciparum 3D7 proteome at the different asexual erythrocytic stages. Protein carbonylation on parasites cultivated in erythrocytes from two donors with HbAS increased 5.34 \u00b1 1.42 folds at the ring stage compared to control grown in hemoglobin A-A (HbAA) red blood cells. Whereas at trophozoites and schizonts stages were augmented 2.80 \u00b1 0.52 and 3.05 \u00b1 0.75 folds, respectively. Besides proteins involved in processes of the stress response, recognition and invasion were identified from schizonts carbonylated bands by combining SDS-PAGE with MALDI-TOF-TOF analysis. Our results reinforce the hypothesis that such oxidative modifications do not appear to happen randomly, and the sickle cell trait affects mainly a small fraction of parasite proteins particularly sensitive to ROS.<\/jats:p>","DOI":"10.3390\/ijms20225769","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T04:31:10Z","timestamp":1574051470000},"page":"5769","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sickle Cell Trait Induces Oxidative Damage on Plasmodium falciparum Proteome at Erythrocyte Stages"],"prefix":"10.3390","volume":"20","author":[{"given":"Alber","family":"D\u00edaz-Castillo","sequence":"first","affiliation":[{"name":"Analytical Chemistry and Biomedicine Group, Exacts and Natural Sciences Faculty, University of Cartagena, 130015 Cartagena, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0974-8894","authenticated-orcid":false,"given":"Neyder","family":"Contreras-Puentes","sequence":"additional","affiliation":[{"name":"Analytical Chemistry and Biomedicine Group, Pharmaceuticals Sciences Faculty, University of Cartagena, 130015 Cartagena, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ciro","family":"Alvear-Sed\u00e1n","sequence":"additional","affiliation":[{"name":"Biochemistry and disease Group, Medicine School, University of Cartagena, 130015 Cartagena, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Moneriz-Pretell","sequence":"additional","affiliation":[{"name":"Biochemistry and disease Group, Medicine School, University of Cartagena, 130015 Cartagena, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Rodr\u00edguez-Cavallo","sequence":"additional","affiliation":[{"name":"Analytical Chemistry and Biomedicine Group, Pharmaceuticals Sciences Faculty, University of Cartagena, 130015 Cartagena, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3905-6859","authenticated-orcid":false,"given":"Dar\u00edo","family":"Mendez-Cuadro","sequence":"additional","affiliation":[{"name":"Analytical Chemistry and Biomedicine Group, Exacts and Natural Sciences Faculty, University of Cartagena, 130015 Cartagena, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.pt.2012.08.003","article-title":"Host actin remodeling and protection from malaria by hemoglobinopathies","volume":"28","author":"Cyrklaff","year":"2012","journal-title":"Trends Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"480","DOI":"10.2471\/BLT.06.036673","article-title":"Global epidemiology of haemoglobin disorders and derived service indicators","volume":"86","author":"Modell","year":"2008","journal-title":"Bull World Health Org."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1002\/pbc.25450","article-title":"Sickle cell in Latin America and the United States [corrected]","volume":"62","author":"Huttle","year":"2015","journal-title":"Pediatr. 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