{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:32:48Z","timestamp":1790695968336,"version":"4.1.0"},"reference-count":140,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2019,11,16]],"date-time":"2019-11-16T00:00:00Z","timestamp":1573862400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004097","name":"Fondation ARC pour la Recherche sur le Cancer","doi-asserted-by":"publisher","award":["DOC20180507400"],"award-info":[{"award-number":["DOC20180507400"]}],"id":[{"id":"10.13039\/501100004097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ongoing progress in genomic technologies offers exciting tools that can help to resolve transcriptome and genome-wide DNA modifications at single-cell resolution. These methods can be used to characterize individual cells within complex tissue organizations and to highlight various molecular interactions. Here, we will discuss recent advances in the definition of spermatogonial stem cells (SSC) and their progenitors in humans using the single-cell transcriptome sequencing (scRNAseq) approach. Exploration of gene expression patterns allows one to investigate stem cell heterogeneity. It leads to tracing the spermatogenic developmental process and its underlying biology, which is highly influenced by the microenvironment. scRNAseq already represents a new diagnostic tool for the personalized investigation of male infertility. One may hope that a better understanding of SSC biology could facilitate the use of these cells in the context of fertility preservation of prepubertal children, as a key component of regenerative medicine.<\/jats:p>","DOI":"10.3390\/ijms20225773","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T04:31:10Z","timestamp":1574051470000},"page":"5773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Contribution of Single-Cell Transcriptomics to the Characterization of Human Spermatogonial Stem Cells: Toward an Application in Male Fertility Regenerative Medicine?"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2427-1994","authenticated-orcid":false,"given":"Anne-Sophie","family":"Gille","sequence":"first","affiliation":[{"name":"UMRE008 Stabilit\u00e9 G\u00e9n\u00e9tique, Cellules Souches et Radiations, Laboratoire des Cellules Souches Germinales, IRCM, Universit\u00e9 de Paris, Universit\u00e9 Paris-Saclay, CEA, F-92260 Fontenay-aux-Roses, France"},{"name":"Team Genomic Epigenetic and Physiopathology of Reproduction, Department of Genetic, Development and Cancer, Cochin Institute, Inserm U1016, 22 rue M\u00e9chain, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mentine","family":"Lapoujade","sequence":"additional","affiliation":[{"name":"UMRE008 Stabilit\u00e9 G\u00e9n\u00e9tique, Cellules Souches et Radiations, Laboratoire des Cellules Souches Germinales, IRCM, Universit\u00e9 de Paris, Universit\u00e9 Paris-Saclay, CEA, F-92260 Fontenay-aux-Roses, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Wolf","sequence":"additional","affiliation":[{"name":"Team Genomic Epigenetic and Physiopathology of Reproduction, Department of Genetic, Development and Cancer, Cochin Institute, Inserm U1016, 22 rue M\u00e9chain, 75014 Paris, France"},{"name":"Sorbonne Paris Cit\u00e9, Faculty of Medicine, University Paris Descartes, Assistance Publique-H\u00f4pitaux de Paris, University Hospital Paris Centre, CHU Cochin, Laboratory of Histology Embryology Biology of Reproduction, 123 boulevard de Port Royal, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Fouchet","sequence":"additional","affiliation":[{"name":"UMRE008 Stabilit\u00e9 G\u00e9n\u00e9tique, Cellules Souches et Radiations, Laboratoire des Cellules Souches Germinales, IRCM, Universit\u00e9 de Paris, Universit\u00e9 Paris-Saclay, CEA, F-92260 Fontenay-aux-Roses, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Barraud-Lange","sequence":"additional","affiliation":[{"name":"Team Genomic Epigenetic and Physiopathology of Reproduction, Department of Genetic, Development and Cancer, Cochin Institute, Inserm U1016, 22 rue M\u00e9chain, 75014 Paris, France"},{"name":"Sorbonne Paris Cit\u00e9, Faculty of Medicine, University Paris Descartes, Assistance Publique-H\u00f4pitaux de Paris, University Hospital Paris Centre, CHU Cochin, Laboratory of Histology Embryology Biology of Reproduction, 123 boulevard de Port Royal, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1126\/science.1247651","article-title":"Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types","volume":"343","author":"Jaitin","year":"2014","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.cels.2016.08.011","article-title":"A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure","volume":"3","author":"Baron","year":"2016","journal-title":"Cell Syst."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/nature13173","article-title":"Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq","volume":"509","author":"Treutlein","year":"2014","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s13059-016-0932-1","article-title":"Single-cell analysis of long non-coding RNAs in the developing human neocortex","volume":"17","author":"Liu","year":"2016","journal-title":"Genome Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nn.4216","article-title":"Adult mouse cortical cell taxonomy revealed by single cell transcriptomics","volume":"19","author":"Tasic","year":"2016","journal-title":"Nat. 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