{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T23:06:21Z","timestamp":1776985581210,"version":"3.51.4"},"reference-count":39,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,11,30]],"date-time":"2019-11-30T00:00:00Z","timestamp":1575072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["UMO-2015\/17\/B\/NZ6\/03473"],"award-info":[{"award-number":["UMO-2015\/17\/B\/NZ6\/03473"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Minister of Science and Higher Education","award":["024\/RID\/2018\/19"],"award-info":[{"award-number":["024\/RID\/2018\/19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Acid sphingomyelinase (aSMase) is involved in the generation of metabolites that function as part of the sphingolipid signaling pathway. It catalyzes the breakdown of sphingomyelin into ceramide, a bioactive lipid that, among other roles, is involved in regulation of apoptosis. Dry drop blood test (DBS) and colorimetric 2-step enzymatic assay were used to assess the activity of human blood aSMase, beta-galactosidase, and beta-glucosidase, these enzymes are lysosomal hydrolases that catalyze the degradation of related sphingolipids, of sphingolipid signaling molecules. Blood was collected from a group of healthy volunteers and patients that were diagnosed with multiple myeloma (MM) in various stages of the disease. Additionally, activity of those enzymes in patients diagnosed with other hematological cancers was also assessed. We found that aSMase activity in the blood of patients with MM (at the time of diagnosis) was 305.43 pmol\/spot*20 h, and this value was significantly lower (p &lt; 0.030) compared to the healthy group 441.88 pmol\/spot*20 h. Our collected data suggest a possible role of aSMase in pathogenesis of MM development.<\/jats:p>","DOI":"10.3390\/ijms20236048","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T10:50:45Z","timestamp":1575283845000},"page":"6048","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Decreased Activity of Blood Acid Sphingomyelinase in the Course of Multiple Myeloma"],"prefix":"10.3390","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3996-200X","authenticated-orcid":false,"given":"Marzena","family":"W\u0105tek","sequence":"first","affiliation":[{"name":"Institute of Hematology and Transfusion Medicine, Indiry Gandhi 14, 02-776 Warsaw, Poland"},{"name":"Department of Microbiology and Immunology, The Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce, Stefana \u017beromskiego 5, 25-001 Kielce, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewelina","family":"Piktel","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2c, 15-222 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Barankiewicz","sequence":"additional","affiliation":[{"name":"Institute of Hematology and Transfusion Medicine, Indiry Gandhi 14, 02-776 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Sierlecka","sequence":"additional","affiliation":[{"name":"Holy Cross Cancer Center, Artwinskiego 4, 25-734 Kielce, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylwia","family":"Ko\u015bcio\u0142ek-Zg\u00f3dka","sequence":"additional","affiliation":[{"name":"Holy Cross Cancer Center, Artwinskiego 4, 25-734 Kielce, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Chabowska","sequence":"additional","affiliation":[{"name":"Regional Blood Transfusion Center in Bialystok, 15-950 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0141ukasz","family":"Suprewicz","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2c, 15-222 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4450-5011","authenticated-orcid":false,"given":"Przemys\u0142aw","family":"Wolak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce, Stefana \u017beromskiego 5, 25-001 Kielce, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bonita","family":"Durna\u015b","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce, Stefana \u017beromskiego 5, 25-001 Kielce, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7664-9226","authenticated-orcid":false,"given":"Robert","family":"Bucki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce, Stefana \u017beromskiego 5, 25-001 Kielce, Poland"},{"name":"Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2c, 15-222 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Lech-Mara\u0144da","sequence":"additional","affiliation":[{"name":"Institute of Hematology and Transfusion Medicine, Indiry Gandhi 14, 02-776 Warsaw, Poland"},{"name":"Centre of Postgraduate Medical Education, Marymoncka 99\/103, 01-813 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1182\/blood-2018-03-840132","article-title":"Identification of novel mutational drivers reveals oncogene dependencies in multiple myeloma","volume":"132","author":"Walker","year":"2018","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s00108-018-0529-8","article-title":"Pathogenesis of multiple myeloma","volume":"60","author":"Rasche","year":"2019","journal-title":"Der Internist"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1158\/1535-7163.MCT-15-0185","article-title":"Sphingosine Kinase-1 Protects Multiple Myeloma from Apoptosis Driven by Cancer-Specific Inhibition of RTKs","volume":"14","author":"Tsukamoto","year":"2015","journal-title":"Mol. 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