{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:51:45Z","timestamp":1760241105226,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T00:00:00Z","timestamp":1575244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016R1A2B2012108 and 2015R1A5A2009124"],"award-info":[{"award-number":["2016R1A2B2012108 and 2015R1A5A2009124"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mollusks have served as important sources of human food and medicine for a long time. Raw Pisidium coreanum, a freshwater bivalve of the phylum Mollusca, is used in traditional therapies in parts of Asia. However, the therapeutic effects of Pisidium coreanum on bone diseases are not known. We investigated the functional roles of Pisidium coreanum in osteoporotic bone diseases. Pisidium coreanum inhibited the differentiation of bone marrow-derived monocytic cells into mature osteoclasts in vitro. The ovariectomized mice that received oral administration of Pisidium coreanum showed improvements in both trabecular and cortical bones. This preventive activity of Pisidium coreanum against bone loss was due to limited osteoclast maturation with reduced osteoclast surface extent in trabecular bone tissue. The formation of large multinucleated osteoclasts in vitro was significantly decreased in response to Pisidium coreanum, consistent with the reduced expression levels of osteoclast markers and fusion-related genes, such as NFATc1, p65, integrin \u03b1v\u03b23, DC-STAMP, OC-STAMP, Atp6v0d2, FAK, CD44, and MFR. These data suggest that Pisidium coreanum inhibits osteoclast differentiation by negatively regulating the fusion of mononuclear osteoclast precursors. Thus, our data demonstrate the ability of Pisidium coreanum to effectively prevent estrogen-deficient osteoporosis through inhibition of multinucleated osteoclast formation.<\/jats:p>","DOI":"10.3390\/ijms20236076","type":"journal-article","created":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T04:58:39Z","timestamp":1575349119000},"page":"6076","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Pisidium coreanum Inhibits Multinucleated Osteoclast Formation and Prevents Estrogen-Deficient Osteoporosis"],"prefix":"10.3390","volume":"20","author":[{"given":"Mun Hwan","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine, Daegu 42415, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyunghee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine, Daegu 42415, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi Yeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine, Daegu 42415, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-In","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology, Institute for Hard Tissue and Bio-Tooth Regeneration, School of Dentistry, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daewon","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine, Daegu 42415, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1111\/j.1469-185X.2010.00124.x","article-title":"Molluscan biological and chemical diversity: Secondary metabolites and medicinal resources produced by marine molluscs","volume":"85","author":"Benkendorff","year":"2010","journal-title":"Biol. 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