{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T15:25:55Z","timestamp":1778253955684,"version":"3.51.4"},"reference-count":50,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2019,12,14]],"date-time":"2019-12-14T00:00:00Z","timestamp":1576281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Lactoferrin (Lf) is an iron-binding glycoprotein protein known to have immune-modulatory role and recently, its anticancerous effect against different cancer cell types was emphasized. In the present investigation, a comparative evaluation of anticancer potential of colostrum-derived lactoferrin from Indian native zebu cow (Sahiwal, SAC), crossbred (Karan Fries, KFC) and commercially available (C-Lf) lactoferrin from exotic cow using cellular models was made. A protocol was standardized successfully to purify Lf protein from colostrum of both breeds using HPLC and purity was confirmed by LC\u2013MS. A standardized dose of 750 \u00b5g\/mL Lf was used to treat two cell types MDA-MB-231 and MCF-7 with Lf from three different sources; SAC-Lf, KFC-Lf and C-Lf for 48 h and 72 h. Different cellular parameters including cytotoxicity, viability, apoptosis and cell proliferation were determined. Comparatively, Lf from commercial source (C-Lf) had maximum effect in both cell types followed by SAC-Lf and KFC-Lf. Further, transcriptional changes in genes associated with apoptosis (Bax and Bcl-2), tumor progression (p53, p21, CD44 and NF-\u03ba\u03b2) and survival (survivin) were evaluated in Lf treatment. The overall results strongly emphasized to the fact that Lf purified from cow colostrum has the capacity to inhibit the in vitro growth of cancerous cell lines albeit to a varied extent.<\/jats:p>","DOI":"10.3390\/ijms20246318","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T05:19:38Z","timestamp":1576473578000},"page":"6318","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of Milk Colostrum Derived Lactoferrin of Sahiwal (Bos indicus) and Karan Fries (Cross-Bred) Cows for Its Anti-Cancerous Potential"],"prefix":"10.3390","volume":"20","author":[{"given":"Ankita","family":"Sharma","sequence":"first","affiliation":[{"name":"ICAR-National Bureau of Animal Genetic Resources, Karnal 132001, India"},{"name":"University Institute of Engineering and Technology, Kurukshetra University, Kurukshetra 136118, India"},{"name":"Department of Animal Biosceinces, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umesh K","family":"Shandilya","sequence":"additional","affiliation":[{"name":"ICAR-National Bureau of Animal Genetic Resources, Karnal 132001, India"},{"name":"Department of Animal Biosceinces, University of Guelph, Guelph, ON N1G 2W1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Sodhi","sequence":"additional","affiliation":[{"name":"ICAR-National Bureau of Animal Genetic Resources, Karnal 132001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashok K","family":"Mohanty","sequence":"additional","affiliation":[{"name":"ICAR-National Dairy Research Institute, Karnal, Haryana 132001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pranay","family":"Jain","sequence":"additional","affiliation":[{"name":"University Institute of Engineering and Technology, Kurukshetra University, Kurukshetra 136118, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manishi","family":"Mukesh","sequence":"additional","affiliation":[{"name":"ICAR-National Bureau of Animal Genetic Resources, Karnal 132001, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"896","DOI":"10.5713\/ajas.2012.12677","article-title":"Effect of cattle breeds on milk composition and technological characteristics in china","volume":"26","author":"Yang","year":"2013","journal-title":"Asian-Austr. 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