{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T13:49:18Z","timestamp":1785592158306,"version":"3.56.0"},"reference-count":30,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T00:00:00Z","timestamp":1576800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>An exopolysaccharides\/calcipotriol (EPS\/CPT) emulsion was prepared using bacterial EPS as emulsifier, sunflower oil as an oil phase and CPT as the loaded drug, and the effect of this emulsion on psoriasis vulgaris treatment was evaluated. An EPS composed of mannose (70.56%) and glucose (29.44%) was obtained from the marine mangrove bacteria Bacillus amyloliquefaciens ZWJ (Zhu Wenjing) strain. The EPS has significant emulsifying activity at the concentration of 1.5%. The prepared EPS\/CPT emulsion has small and stable particle size, with a drug content of 0.00492%, and good spreading properties. The in vitro drug release results revealed that the emulsion showed a certain sustained release effect. In vitro and in vivo animal experiments show that the EPS\/CPT emulsion can effectively treat psoriasis vulgaris by increasing the accumulation of CPT in psoriatic skin lesions and reducing the levels of inflammatory cells and inflammatory factors (TNF and IL6). Additionally, it has a certain effect on reducing the side effects associated with CPT. This study lays a foundation for the research of EPS in the topical application of medical materials and treatment of psoriasis.<\/jats:p>","DOI":"10.3390\/ijms21010077","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T09:50:33Z","timestamp":1576835433000},"page":"77","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Preparation of Calcipotriol Emulsion Using Bacterial Exopolysaccharides as Emulsifier for Percutaneous Treatment of Psoriasis Vulgaris"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3747-5491","authenticated-orcid":false,"given":"Bo","family":"Song","sequence":"first","affiliation":[{"name":"School of Pharmacy, Weifang Medical University, Weifang 261042, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiteng","family":"Song","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Weifang Medical University, Weifang 261042, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Cheng","sequence":"additional","affiliation":[{"name":"Clinical Medical School, Weifang Medical University, Weifang 261042, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hairong","family":"Chu","sequence":"additional","affiliation":[{"name":"Clinical Medical School, Weifang Medical University, Weifang 261042, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Weifang Medical University, Weifang 261042, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Weifang Medical University, Weifang 261042, Shandong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1038\/492S64a","article-title":"Under their skin","volume":"492","author":"Margaret","year":"2012","journal-title":"Natrue"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jaad.2018.06.056","article-title":"Psoriasis: Which therapy for which patient focus on special populations and chronic infections","volume":"80","author":"Kaushik","year":"2019","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"737","DOI":"10.4155\/tde-2017-0058","article-title":"Calcipotriene betamethasone dipropionate aerosol foam in the treatment of plaque psoriasis: A review of the literature","volume":"8","author":"Frieder","year":"2017","journal-title":"Ther. Deliv."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1016\/j.nano.2017.02.009","article-title":"Nanoemulsion loaded gel for topical co-delivery of clobitasol propionate and calcipotriol in psoriasis","volume":"13","author":"Kaur","year":"2017","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jconrel.2017.11.049","article-title":"Nanoemulsion as pharmaceutical carrier for dermal and transdermal drug delivery: Formulation development, stability issues, basic considerations and applications","volume":"270","author":"Rai","year":"2018","journal-title":"J. Control. Release Off. J. Control. Release Soc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.ijpharm.2014.10.067","article-title":"New topical formulation for psoriasis: Development of methotrexate-loaded nanostructured lipid carriers","volume":"477","author":"Pinto","year":"2014","journal-title":"Int. J. Pharm."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.ejps.2018.04.029","article-title":"Delivery of adapalene using a novel topical gel based on tea tree oil nano-emulsion: Permeation, antibacterial and safety assessments","volume":"120","author":"Ghaemi","year":"2018","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1016\/j.carbpol.2014.08.060","article-title":"New emulsifying and cryoprotective exopolysaccharide from Antarctic pseudomonas, sp. id1","volume":"117","author":"Delgado","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.biotechadv.2010.02.006","article-title":"Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms","volume":"28","author":"Satpute","year":"2010","journal-title":"Biotechnol. Adv."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.imlet.2014.11.008","article-title":"Exopolysaccharides from Cyanobacterium aponinum from the Blue Lagoon in Iceland increase IL-10 secretion by human dendritic cells and their ability to reduce the IL-17+ROR\u00ce\u00b3t+\/IL-10+FoxP3+ ratio in CD4+ T cells","volume":"163","author":"Gudmundsdottir","year":"2015","journal-title":"Immunol. Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7141","DOI":"10.1007\/s00253-013-5000-0","article-title":"Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic","volume":"97","author":"Song","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1007\/s00284-014-0717-2","article-title":"Two Novel Exopolysaccharides from Bacillus amyloliquefaciens C-1: Antioxidation and Effect on Oxidative Stress","volume":"70","author":"Yang","year":"2015","journal-title":"Curr. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.carbpol.2014.08.044","article-title":"Rheological, emulsifying and thermostability properties of two exopolysaccharides produced by Bacillus amyloliquefaciens lpl061","volume":"115","author":"Han","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6398","DOI":"10.1039\/b709807n","article-title":"Effect of electrolyte in silicone oil-in-water emulsions stabilised by fumed silica particles","volume":"9","author":"Horozov","year":"2007","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.carbpol.2012.05.075","article-title":"Emulsifying, rheological and physicochemical properties of exopolysaccharide produced by Bifidobacterium longum subsp. infantis CCUG 52486 and Bifidobacterium infantis NCIMB 702205","volume":"90","author":"Prasanna","year":"2012","journal-title":"Carbohyd. Polym."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.foodres.2009.02.007","article-title":"An exopolysaccharide from a probiotic: Biosynthesis dynamics, composition and emulsifying activity","volume":"42","author":"Kodali","year":"2009","journal-title":"Food Res. Int."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.enzmictec.2005.06.007","article-title":"Emulsifying properties of a marine bacterial exopolysaccharide","volume":"38","author":"Iyer","year":"2006","journal-title":"Enzyme Microb. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.carbpol.2015.08.038","article-title":"Pinh\u00e3o starch and coat extract as new natural cosmetic ingredients: Topical formulation stability and sensory analysis","volume":"13","author":"Daudt","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.colsurfb.2017.03.028","article-title":"Spreading properties of cosmetic emollients: Use of synthetic skin surface to elucidate structural effect","volume":"154","author":"Douguet","year":"2017","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"42","DOI":"10.14227\/DT190112P42","article-title":"In vitro dissolution kinetics of captopril from microspheres manufactured by solvent evaporation","volume":"19","author":"Khamanga","year":"2012","journal-title":"Dissolut. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1517\/17425247.2014.938634","article-title":"Solid lipid nanoparticles-loaded topical gel containing combination drugs: An approach to offset psoriasis","volume":"11","author":"Sonawane","year":"2014","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1046\/j.1365-4362.1999.00622.x","article-title":"Cytokines in psoriasis","volume":"38","author":"Bonifati","year":"2010","journal-title":"Int. J. Derm."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6892","DOI":"10.3748\/wjg.v21.i22.6892","article-title":"Nonalcoholic fatty liver disease, spleen and psoriasis: New aspects of low-grade chronic inflammation","volume":"21","author":"Balato","year":"2015","journal-title":"World J. Gastroenterol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1111\/bjd.14236","article-title":"Surface air plasma-induced cell death and cytokine release of human keratinocytes in the context of psoriasis","volume":"174","author":"Zhong","year":"2016","journal-title":"Br. J. Derm."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1038\/nature05505","article-title":"Interleukin-22, a th17 cytokine, mediates il-23-induced dermal inflammation and acanthosis","volume":"445","author":"Zheng","year":"2007","journal-title":"Nature"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.jconrel.2013.05.020","article-title":"Novel colloidal carriers for psoriasis: Current issues, mechanistic insight and novel delivery approaches","volume":"170","author":"Pradhan","year":"2013","journal-title":"J. Control. Release Off. J. Control. Release Soc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.carbpol.2018.03.063","article-title":"Physical and chemical properties, percutaneous absorption-promoting effects of exopolysaccharide produced by Bacillus atrophaeus WYZ strain","volume":"192","author":"Zhu","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_28","unstructured":"Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Preparation and analysis of eukaryotic genomic DNA. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press. [2nd ed.]."},{"key":"ref_29","first-page":"534","article-title":"Study on Determination of Reducing Sugar Content Using 3,5-Dinitrosalicylic Acid Method","volume":"29","author":"Zhao","year":"2008","journal-title":"Food Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jfoodeng.2016.09.006","article-title":"Emulsifier free emulsion: Comparative study between a new high frequency ultrasound process and standard emulsification processes","volume":"194","author":"Kaci","year":"2016","journal-title":"J. Food Eng."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/1\/77\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:44:15Z","timestamp":1760190255000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/1\/77"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,20]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["ijms21010077"],"URL":"https:\/\/doi.org\/10.3390\/ijms21010077","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,20]]}}}