{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:48:13Z","timestamp":1790833693361,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T00:00:00Z","timestamp":1577404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-HL146691"],"award-info":[{"award-number":["R01-HL146691"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"nih","doi-asserted-by":"publisher","award":["R01-DK033823"],"award-info":[{"award-number":["R01-DK033823"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The inclusion of microRNAs (miRNAs) in extracellular microvesicles\/exosomes (named cardiosomes when deriving from cardiomyocytes) allows their active transportation and ensures cell-cell communication. We hypothesize that cardiosomal miRNAs play a pivotal role in the activation of myofibroblasts following ischemic injury. Using a murine model of myocardial infarction (MI), we tested our hypothesis by measuring in isolated fibroblasts and cardiosomes the expression levels of a set of miRNAs, which are upregulated in cardiomyocytes post-MI and involved in myofibroblast phenoconversion. We found that miR-195 was significantly upregulated in cardiosomes and in fibroblasts isolated after MI compared with SHAM conditions. Moreover, primary isolated cardiac fibroblasts were activated both when incubated with cardiosomes isolated from ischemic cardiomyocytes and when cultured in conditioned medium of post-MI cardiomyocytes, whereas no significant effect was observed following incubation with cardiosomes or medium from sham cardiomyocytes. Taken together, our findings indicate for the first time that a cardiomyocyte-specific miRNA, transferred to fibroblasts in form of exosomal cargo, is crucial in the activation of myofibroblasts.<\/jats:p>","DOI":"10.3390\/ijms21010201","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T11:42:47Z","timestamp":1577446967000},"page":"201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["Cardiosomal microRNAs Are Essential in Post-Infarction Myofibroblast Phenoconversion"],"prefix":"10.3390","volume":"21","author":[{"given":"Marco B.","family":"Morelli","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Cardiology and Department of Molecular Pharmacology, Fleischer Institute for Diabetes and Metabolism (FIDAM), Albert Einstein College of Medicine, Montefiore University Hospital, New York, NY 10461, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Cardiology and Department of Molecular Pharmacology, Fleischer Institute for Diabetes and Metabolism (FIDAM), Albert Einstein College of Medicine, Montefiore University Hospital, New York, NY 10461, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celestino","family":"Sardu","sequence":"additional","affiliation":[{"name":"Department of Medical, Surgical, Neurological, Metabolic and Aging Sciences, University of Campania \u201cLuigi Vanvitelli\u201d, 80100 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Matarese","sequence":"additional","affiliation":[{"name":"Department of Pneumology and Oncology, AORN \u201cOspedale dei Colli\u201d, 80100 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7231-375X","authenticated-orcid":false,"given":"Gaetano","family":"Santulli","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Cardiology and Department of Molecular Pharmacology, Fleischer Institute for Diabetes and Metabolism (FIDAM), Albert Einstein College of Medicine, Montefiore University Hospital, New York, NY 10461, USA"},{"name":"Department of Advanced Biomedical Science, \u201cFederico II\u201d University, and International Translational Research and Medical Education Consortium (ITME), 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1161\/CIRCRESAHA.118.314438","article-title":"Protective Effects of Activated Myofibroblasts in the Pressure-Overloaded Myocardium Are Mediated Through Smad-Dependent Activation of a Matrix-Preserving Program","volume":"8","author":"Russo","year":"2019","journal-title":"Circ. 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