{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:31:07Z","timestamp":1789029067079,"version":"build-2803163510"},"reference-count":72,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T00:00:00Z","timestamp":1577750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI12\/00729"],"award-info":[{"award-number":["PI12\/00729"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015528","name":"Universidad de La Laguna","doi-asserted-by":"publisher","award":["Proyecto Puente al Plan Estatal 2018"],"award-info":[{"award-number":["Proyecto Puente al Plan Estatal 2018"]}],"id":[{"id":"10.13039\/100015528","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agencia Canaria de Investigaci\u00f3n, Innovaci\u00f3n y Sociedad de la Informaci\u00f3n (ACIISI)","award":["FPI PhD to E.M-R"],"award-info":[{"award-number":["FPI PhD to E.M-R"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sirtuins are a family of deacetylases that modify structural proteins, metabolic enzymes, and histones to change cellular protein localization and function. In mammals, there are seven sirtuins involved in processes like oxidative stress or metabolic homeostasis associated with aging, degeneration or cancer. We studied gene expression of sirtuins by qRT-PCR in human mural granulosa-lutein cells (hGL) from IVF patients in different infertility diagnostic groups and in oocyte donors (OD; control group). Study 1: sirtuins genes\u2019 expression levels and correlations with age and IVF parameters in women with no ovarian factor. We found significantly higher expression levels of SIRT1, SIRT2 and SIRT5 in patients \u226540 years old than in OD and in women between 27 and 39 years old with tubal or male factor, and no ovarian factor (NOF). Only SIRT2, SIRT5 and SIRT7 expression correlated with age. Study 2: sirtuin genes\u2019 expression in women poor responders (PR), endometriosis (EM) and polycystic ovarian syndrome. Compared to NOF controls, we found higher SIRT2 gene expression in all diagnostic groups while SIRT3, SIRT5, SIRT6 and SIRT7 expression were higher only in PR. Related to clinical parameters SIRT1, SIRT6 and SIRT7 correlate positively with FSH and LH doses administered in EM patients. The number of mature oocytes retrieved in PR is positively correlated with the expression levels of SIRT3, SIRT4 and SIRT5. These data suggest that cellular physiopathology in PR\u2019s follicle may be associated with cumulative DNA damage, indicating that further studies are necessary.<\/jats:p>","DOI":"10.3390\/ijms21010295","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T04:43:03Z","timestamp":1578026583000},"page":"295","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Granulosa-Lutein Cell Sirtuin Gene Expression Profiles Differ between Normal Donors and Infertile Women"],"prefix":"10.3390","volume":"21","author":[{"given":"Rebeca","family":"Gonz\u00e1lez-Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda del Desarrollo, UD de Bioqu\u00edmica y Biolog\u00eda Molecular and Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna, Av. Astrof\u00edsico S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Mart\u00edn-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda del Desarrollo, UD de Bioqu\u00edmica y Biolog\u00eda Molecular and Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna, Av. Astrof\u00edsico S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9760-4211","authenticated-orcid":false,"given":"Deborah","family":"Rotoli","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda del Desarrollo, UD de Bioqu\u00edmica y Biolog\u00eda Molecular and Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna, Av. Astrof\u00edsico S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"},{"name":"Institute of Endocrinology and Experimental Oncology (IEOS), CNR-National Research Council, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jairo","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Centro de Asistencia a la Reproducci\u00f3n Humana de Canarias, 38202 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick","family":"Naftolin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, New York University, New York, NY 10016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2220-5774","authenticated-orcid":false,"given":"Pablo","family":"Mart\u00edn-Vasallo","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda del Desarrollo, UD de Bioqu\u00edmica y Biolog\u00eda Molecular and Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna, Av. Astrof\u00edsico S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Palumbo","sequence":"additional","affiliation":[{"name":"Centro de Asistencia a la Reproducci\u00f3n Humana de Canarias, 38202 La Laguna, Tenerife, Spain"},{"name":"Department of Obstetrics and Gynecology, New York University, New York, NY 10016, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5177-1188","authenticated-orcid":false,"given":"Julio","family":"\u00c1vila","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda del Desarrollo, UD de Bioqu\u00edmica y Biolog\u00eda Molecular and Centro de Investigaciones Biom\u00e9dicas de Canarias (CIBICAN), Universidad de La Laguna, Av. Astrof\u00edsico S\u00e1nchez s\/n, 38206 La Laguna, Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.freeradbiomed.2012.10.525","article-title":"Seven sirtuins for seven deadly diseases of aging","volume":"56","author":"Morris","year":"2013","journal-title":"Free Radic. Biol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1002\/med.20178","article-title":"NAD (+) -dependent histone deacetylases (sirtuins) as novel therapeutic targets","volume":"30","author":"Schemies","year":"2010","journal-title":"Med. Res. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.molmed.2017.02.005","article-title":"Mitochondrial Sirtuins and Molecular Mechanisms of Aging","volume":"23","author":"Santos","year":"2017","journal-title":"Trends Mol. 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