{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:44:40Z","timestamp":1762659880424,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T00:00:00Z","timestamp":1578182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["FS\/15\/46\/31606"],"award-info":[{"award-number":["FS\/15\/46\/31606"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the actin cytoskeleton, represented by the abundant coronins 1, 2, and 3 and the less abundant coronin 7 in platelets, but their functions in these cells are poorly understood. A recent report revealed impaired agonist-induced actin polymerization and cofilin phosphoregulation and altered thrombus formation in vivo as salient phenotypes in the absence of an overt hemostasis defect in vivo in a knockout mouse model of coronin 1. Here we show that the absence of coronin 1 is associated with impaired translocation of integrin \u03b22 to the platelet surface upon stimulation with thrombin while morphological and functional alterations, including defects in Arp2\/3 complex localization and cAMP-dependent signaling, are absent. Our results suggest a large extent of functional overlap among coronins 1, 2, and 3 in platelets, while aspects like integrin \u03b22 translocation are specifically or predominantly dependent on coronin 1.<\/jats:p>","DOI":"10.3390\/ijms21010356","type":"journal-article","created":{"date-parts":[[2020,1,6]],"date-time":"2020-01-06T03:48:48Z","timestamp":1578282528000},"page":"356","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Coronin 1 Is Required for Integrin \u03b22 Translocation in Platelets"],"prefix":"10.3390","volume":"21","author":[{"given":"David R. J.","family":"Riley","sequence":"first","affiliation":[{"name":"Centre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jawad S.","family":"Khalil","sequence":"additional","affiliation":[{"name":"Centre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK"},{"name":"School of Physiology, Pharmacology and Neuroscience, Faculty of Life Sciences, University of Bristol, Bristol BS8 1TD, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Pieters","sequence":"additional","affiliation":[{"name":"Biozentrum, University of Basel, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalid M.","family":"Naseem","sequence":"additional","affiliation":[{"name":"Leeds Institute for Cardiovascular and Metabolic Medicine, University of Leeds, Leeds LS2 9NL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5435-6991","authenticated-orcid":false,"given":"Francisco","family":"Rivero","sequence":"additional","affiliation":[{"name":"Centre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,5]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Regulation of platelet activation and coagulation and its role in vascular injury and arterial thrombosis","volume":"6","author":"Tomaiuolo","year":"2017","journal-title":"Interv. Cardiol. Clin."},{"key":"ref_2","unstructured":"Michelson, A.D. (2013). The platelet cytoskeleton. Platelets, Academic Press. [3rd ed.]."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/j.1538-7836.2011.04576.x","article-title":"Novel roles of cAMP\/cGMP-dependent signaling in platelets","volume":"10","author":"Smolenski","year":"2012","journal-title":"J. Thromb. Haemost."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.tcb.2011.04.004","article-title":"Unraveling the enigma: Progress towards understanding the coronin family of actin regulators","volume":"21","author":"Chan","year":"2011","journal-title":"Trends Cell Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"23765056","DOI":"10.1038\/nri3465","article-title":"On guard: Coronin proteins in innate and adaptive immunity","volume":"13","author":"Pieters","year":"2013","journal-title":"Nat. Rev. Immunol."},{"key":"ref_6","first-page":"1","article-title":"Biochemical and immunocytochemical characterization of coronins in platelets","volume":"4","author":"Riley","year":"2019","journal-title":"Platelets"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0014-5793(95)00393-N","article-title":"Molecular cloning of a novel actin-binding protein, p57, with a WD repeat and a leucine zipper motif","volume":"364","author":"Suzuki","year":"1995","journal-title":"FEBS Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0092-8674(00)80754-0","article-title":"A coat protein on phagosomes involved in the intracellular survival of mycobacteria","volume":"97","author":"Ferrari","year":"1999","journal-title":"Cell"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Jayachandran, R., Liu, X., Bosedasgupta, S., M\u00fcller, P., Zhang, C.-L., Moshous, D., Studer, V., Schneider, J., Genoud, C., and Fossoud, C. (2014). Coronin 1 regulates cognition and behavior through modulation of cAMP\/protein kinase A signaling. PLoS Biol., 12.","DOI":"10.1371\/journal.pbio.1001820"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1182\/blood-2016-11-749622","article-title":"Coronin 1A, a novel player in integrin biology, controls neutrophil trafficking in innate immunity","volume":"130","author":"Pick","year":"2017","journal-title":"Blood"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3388","DOI":"10.1128\/MCB.00347-14","article-title":"Coronin1 proteins dictate Rac1 intracellular dynamics and cytoskeletal output","volume":"34","author":"Ojeda","year":"2014","journal-title":"Mol. Cell. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.4049\/jimmunol.0801811","article-title":"Coronin 1 is essential for IgM-mediated Ca2+ mobilization in B cells but dispensable for the generation of immune responses in vivo","volume":"182","author":"Combaluzier","year":"2009","journal-title":"J. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1242\/jcs.110.24.3071","article-title":"Cytosolic phox proteins interact with and regulate the assembly of coronin in neutrophils","volume":"110","author":"Grogan","year":"1997","journal-title":"J. Cell Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7254","DOI":"10.4049\/jimmunol.0803312","article-title":"Coronin-1 is associated with neutrophil survival and is cleaved during apoptosis: Potential implication in neutrophils from cystic fibrosis patients","volume":"182","author":"Moriceau","year":"2009","journal-title":"J. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.cell.2007.04.043","article-title":"Survival of mycobacteria in macrophages is mediated by coronin 1-dependent activation of calcineurin","volume":"130","author":"Jayachandran","year":"2007","journal-title":"Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1038\/ni1570","article-title":"Regulation of T cell survival through coronin-1-mediated generation of inositol-1,4,5-trisphosphate and calcium mobilization after T cell receptor triggering","volume":"9","author":"Mueller","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1126\/science.1130563","article-title":"Requirement for coronin 1 in T lymphocyte trafficking and cellular homeostasis","volume":"313","author":"Rangell","year":"2006","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1038\/ni.1662","article-title":"The actin regulator coronin 1A is mutant in a thymic egress-deficient mouse strain and in a patient with severe combined immunodeficiency","volume":"9","author":"Shiow","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.immuni.2007.11.023","article-title":"The lupus-related Lmb3 locus contains a disease-suppressing Coronin-1A gene mutation","volume":"28","author":"Haraldsson","year":"2009","journal-title":"Immunity"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1055\/s-0038-1675604","article-title":"The actin regulator coronin-1A modulates platelet shape change and consolidates arterial thrombosis","volume":"118","author":"Stocker","year":"2018","journal-title":"Thromb. Haemost."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"11877","DOI":"10.1073\/pnas.93.21.11877","article-title":"In vivo tracking of platelets: Circulating degranulated platelets rapidly lose surface P-selectin but continue to circulate and function","volume":"93","author":"Michelson","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4182","DOI":"10.1128\/iai.61.10.4182-4187.1993","article-title":"An effector role for platelets in systemic and local lipopolysaccharide\u2014Induced toxicity in mice, mediated by a CD11a- and CD54-dependent interaction with endothelium","volume":"61","author":"Piguet","year":"1993","journal-title":"Infect. Immun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1046\/j.1365-2141.1998.00618.x","article-title":"Stimulation of thrombocytopoiesis decreases platelet beta2 but not beta1 or beta3 integrins","volume":"100","author":"Guo","year":"1998","journal-title":"Br. J. Haematol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1078\/0171-9335-00147","article-title":"Beta2 integrin modulates platelet caspase activation and life span in mice","volume":"80","author":"Piguet","year":"2001","journal-title":"Eur. J. Cell Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1074\/mcp.M114.038513","article-title":"Copy number analysis of the murine platelet proteome spanning the complete abundance range","volume":"13","author":"Zeiler","year":"2014","journal-title":"Mol. Cell. Proteom."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1600-0609.1996.tb01331.x","article-title":"Activated human platelets express \u03b22 integrin","volume":"56","author":"Philippeaux","year":"2009","journal-title":"Eur. J. Haematol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0022-1759(00)00192-7","article-title":"A surrogate method for assessment of \u03b22-integrin-dependent adhesion of human eosinophils to ICAM-1","volume":"240","author":"Zhu","year":"2000","journal-title":"J. Immunol. Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.pupt.2003.10.005","article-title":"\u03b22-Integrin adhesion caused by eotaxin but not IL-5 is blocked by PDE-4 inhibition and \u03b22-adrenoceptor activation in human eosinophils","volume":"17","author":"Liu","year":"2004","journal-title":"Pulm. Pharmacol. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.immuni.2018.12.011","article-title":"Disruption of coronin 1 signaling in T cells promotes allograft tolerance while maintaining anti-pathogen immunity","volume":"50","author":"Jayachandran","year":"2019","journal-title":"Immunity"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Graesele, P., Kleiman, N.S., Lopez, J.A., and Page, C.P. (2017). Anatomy of the platelet cytoskeleton. Platelets in Thrombotic and Non-Thrombotic Disorders, Springer.","DOI":"10.1007\/978-3-319-47462-5"},{"key":"ref_31","first-page":"65","article-title":"Platelet aggregation: Turbidimetric measurements","volume":"272","author":"Jarvis","year":"2004","journal-title":"Methods Mol. Biol."},{"key":"ref_32","first-page":"77","article-title":"Platelet aggregation in whole blood: Impedance and particle counting methods","volume":"272","author":"Jarvis","year":"2004","journal-title":"Methods Mol. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2019.00254","article-title":"Beta2-Integins and interacting proteins in leukocyte trafficking, immune supression, and immunodeficiency disease","volume":"10","author":"Fagerholm","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e2010023","DOI":"10.4084\/mjhid.2010.023","article-title":"Platelet-leukocyte interactions: Multiple links between inflammation, blood coagulation and vascular risk","volume":"2","author":"Cerletti","year":"2010","journal-title":"Mediterr. J. Hematol. Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/1873-3468.12046","article-title":"Activation of the cAMP\/protein kinase A signalling pathway by coronin 1 is regulated by cyclin-dependent kinase 5 activity","volume":"590","author":"Liu","year":"2016","journal-title":"FEBS Lett."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1091\/mbc.e13-04-0219","article-title":"Initiation of multicellular differentiation in Dictyostelium discoideum is regulated by coronin A","volume":"25","author":"Vinet","year":"2014","journal-title":"Mol. Biol. Cell"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Li, X., Zhong, K., Yin, Z., Hu, J., Wang, W., Li, L., Zhang, H., Zheng, X., Wang, P., and Zhang, Z. (2019). The seven transmembrane domain protein MoRgs7 functions in surface perception and undergoes coronin MoCrn1-dependent endocytosis in complex with G\u03b1 subunit MoMagA to promote cAMP signaling and appressorium formation in Magnaporthe oryzae. PLoS Pathog., 15.","DOI":"10.1101\/435511"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/978-3-642-29423-5_9","article-title":"Targeting phosphodiesterases in anti-platelet therapy","volume":"210","author":"Rondina","year":"2012","journal-title":"Handb. Exp. Pharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e73","DOI":"10.1182\/blood-2012-04-416594","article-title":"The first comprehensive and quantitative analysis of human platelet protein composition allows the comparative analysis of structural and functional pathways","volume":"120","author":"Burkhart","year":"2012","journal-title":"Blood"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1080\/09537104.2016.1235688","article-title":"The actin binding proteins cortactin and HS1 are dispensable for platelet actin nodule and megakaryocyte podosome formation","volume":"28","author":"Thomas","year":"2017","journal-title":"Platelets"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1080\/09537104.2018.1481937","article-title":"Formin proteins in megakaryocytes and platelets: Regulation of actin and microtubule dynamics","volume":"30","author":"Zuidscherwoude","year":"2019","journal-title":"Platelets"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"48858","DOI":"10.1074\/jbc.M205136200","article-title":"Oligomerization, F-actin interaction, and membrane association of the ubiquitous mammalian coronin 3 are mediated by its carboxyl terminus","volume":"277","author":"Spoerl","year":"2002","journal-title":"J. Biol. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/1\/356\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:42:21Z","timestamp":1760362941000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/1\/356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,5]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["ijms21010356"],"URL":"https:\/\/doi.org\/10.3390\/ijms21010356","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2020,1,5]]}}}