{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:29:50Z","timestamp":1790810990044,"version":"4.1.0"},"reference-count":54,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,11]],"date-time":"2020-01-11T00:00:00Z","timestamp":1578700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Evolution","award":["No grant number"],"award-info":[{"award-number":["No grant number"]}]},{"DOI":"10.13039\/501100003032","name":"Association Nationale de la Recherche et de la Technologie","doi-asserted-by":"publisher","award":["2017\/0684"],"award-info":[{"award-number":["2017\/0684"]}],"id":[{"id":"10.13039\/501100003032","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The bovine embryo develops in contact with the oviductal fluid (OF) during the first 4\u20135 days of pregnancy. The aim of this study was to decipher the protein interactions occurring between the developing embryo and surrounding OF. In-vitro produced 4\u20136 cell and morula embryos were incubated or not (controls) in post-ovulatory OF (OF-treated embryos) and proteins were then analyzed and quantified by high resolution mass spectrometry (MS) in both embryo groups and in OF. A comparative analysis of MS data allowed the identification and quantification of 56 embryo-interacting proteins originated from the OF, including oviductin (OVGP1) and several annexins (ANXA1, ANXA2, ANXA4) as the most abundant ones. Some embryo-interacting proteins were developmental stage-specific, showing a modulating role of the embryo in protein interactions. Three interacting proteins (OVGP1, ANXA1 and PYGL) were immunolocalized in the perivitelline space and in blastomeres, showing that OF proteins were able to cross the zona pellucida and be taken up by the embryo. Interacting proteins were involved in a wide range of functions, among which metabolism and cellular processes were predominant. This study identified for the first time a high number of oviductal embryo-interacting proteins, paving the way for further targeted studies of proteins potentially involved in the establishment of pregnancy in cattle.<\/jats:p>","DOI":"10.3390\/ijms21020466","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T04:05:51Z","timestamp":1578888351000},"page":"466","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Identification of 56 Proteins Involved in Embryo\u2013Maternal Interactions in the Bovine Oviduct"],"prefix":"10.3390","volume":"21","author":[{"given":"Charles","family":"Banliat","sequence":"first","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, IFCE, UMR PRC, 37380 Nouzilly, France"},{"name":"Union Evolution, 35530 Noyal-sur-Vilaine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Tsikis","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, IFCE, UMR PRC, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Labas","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, IFCE, UMR PRC, 37380 Nouzilly, France"},{"name":"INRAE, Universit\u00e9 de Tours, CHU de Tours, Plate-forme CIRE, PAIB, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana-Paula","family":"Teixeira-Gomes","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 de Tours, CHU de Tours, Plate-forme CIRE, PAIB, 37380 Nouzilly, France"},{"name":"INRAE, UMR 1282 ISP, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Com","sequence":"additional","affiliation":[{"name":"Inserm, University of Rennes, EHESP, Irset (Institut de recherche en sant\u00e9, environnement et travail)\u2014UMR_S 1085, 35000 Rennes, France"},{"name":"Protim, Inserm U1085, Irset, Campus de Beaulieu, University of Rennes 1, Proteomics Core Facility, 35000 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9gis","family":"Lavigne","sequence":"additional","affiliation":[{"name":"Inserm, University of Rennes, EHESP, Irset (Institut de recherche en sant\u00e9, environnement et travail)\u2014UMR_S 1085, 35000 Rennes, France"},{"name":"Protim, Inserm U1085, Irset, Campus de Beaulieu, University of Rennes 1, Proteomics Core Facility, 35000 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Pineau","sequence":"additional","affiliation":[{"name":"Inserm, University of Rennes, EHESP, Irset (Institut de recherche en sant\u00e9, environnement et travail)\u2014UMR_S 1085, 35000 Rennes, France"},{"name":"Protim, Inserm U1085, Irset, Campus de Beaulieu, University of Rennes 1, Proteomics Core Facility, 35000 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Guyonnet","sequence":"additional","affiliation":[{"name":"Union Evolution, 35530 Noyal-sur-Vilaine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9836-2506","authenticated-orcid":false,"given":"Pascal","family":"Mermillod","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, IFCE, UMR PRC, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4569-5665","authenticated-orcid":false,"given":"Marie","family":"Saint-Dizier","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Universit\u00e9 de Tours, IFCE, UMR PRC, 37380 Nouzilly, France"},{"name":"Faculty of Sciences and Techniques, Department Agrosciences, University of Tours, 37000 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1095\/biolreprod.108.073874","article-title":"Ciliary transport, gamete interaction, and effects of the early embryo in the oviduct: Ex vivo analyses using a new digital videomicroscopic system in the cow","volume":"81","author":"Kolle","year":"2009","journal-title":"Biol. 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