{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:27:07Z","timestamp":1790749627980,"version":"4.1.0"},"reference-count":75,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T00:00:00Z","timestamp":1579219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We have identified a Holstein sire named Tarantino who had been approved for artificial insemination that is based on normal semen characteristics (i.e., morphology, thermoresistance, motility, sperm concentration), but had no progeny after 412 first inseminations, resulting in a non-return rate (NRdev) of \u221229. Using whole genome association analysis and next generation sequencing, an associated nonsense variant in the \u03b1\/\u03b2-hydrolase domain-containing 16B gene (ABHD16B) on bovine chromosome 13 was identified. The frequency of the mutant allele in the German Holstein population was determined to be 0.0018 in 222,645 investigated cattle specimens. The mutant allele was traced back to Whirlhill Kingpin (bornFeb. 13th, 1959) as potential founder. The expression of ABHD16B was detected by Western blotting and immunohistochemistry in testis and epididymis of control bulls. A lipidome comparison of the plasma membrane of fresh semen from carriers and controls showed significant differences in the concentration of phosphatidylcholine (PC), diacylglycerol (DAG), ceramide (Cer), sphingomyelin (SM), and phosphatidylcholine (-ether) (PC O-), indicating that ABHD16B plays a role in lipid biosynthesis. The altered lipid contents may explain the reduced fertilization ability of mutated sperms.<\/jats:p>","DOI":"10.3390\/ijms21020627","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T04:27:09Z","timestamp":1579494429000},"page":"627","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Association of \u03b1\/\u03b2-Hydrolase D16B with Bovine Conception Rate and Sperm Plasma Membrane Lipid Composition"],"prefix":"10.3390","volume":"21","author":[{"given":"Shuwen","family":"Shan","sequence":"first","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangzheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Bleyer","sequence":"additional","affiliation":[{"name":"Pathology Unit, German Primate Center, Leibniz-Institute for Primate Research Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svenja","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torben","family":"Melbaum","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelm","family":"Wemheuer","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4796-024X","authenticated-orcid":false,"given":"Marc","family":"Hirschfeld","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"},{"name":"Department of Obstetrics and Gynecology, University Medical Center Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christin","family":"Wacker","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Lab of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekkehard","family":"Sch\u00fctz","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7635-9656","authenticated-orcid":false,"given":"Bertram","family":"Brenig","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of Goettingen, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1262\/jrd.10-197E","article-title":"Practical aspects of the fertility of dairy cattle","volume":"57","author":"Sakaguchi","year":"2011","journal-title":"J. Reprod. Dev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S266","DOI":"10.1016\/j.theriogenology.2007.04.034","article-title":"Genetics and genomics to improve fertility in high producing dairy cows","volume":"68","author":"Veerkamp","year":"2007","journal-title":"Theriogenology"},{"key":"ref_3","first-page":"237","article-title":"Fertility in high-producing dairy cows: Reasons for decline and corrective strategies for sustainable improvement","volume":"64","author":"Lucy","year":"2007","journal-title":"Soc. Reprod. Fertil. Suppl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4082","DOI":"10.3168\/jds.2007-0858","article-title":"Economic analysis of reproductive management strategies on US commercial dairy farms","volume":"91","author":"Olynk","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Harstine, B.R., Utt, M.D., and DeJarnette, J.M. (2018). Review: Integrating a semen quality control program and sire fertility at a large artificial insemination organization. Animal, 1\u201312.","DOI":"10.1017\/S1751731118000319"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.anireprosci.2017.03.022","article-title":"Toward an integrative and predictive sperm quality analysis in Bos taurus","volume":"181","author":"Yaniz","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.anireprosci.2017.08.001","article-title":"Sperm quality variables as indicators of bull fertility may be breed dependent","volume":"185","author":"Morrell","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1111\/rda.12747","article-title":"Genomewide analysis of bull sperm quality and fertility traits","volume":"51","author":"Puglisi","year":"2016","journal-title":"Reprod. Domest. Anim."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.theriogenology.2013.09.001","article-title":"Understanding and evaluating bovine testes","volume":"81","author":"Kastelic","year":"2014","journal-title":"Theriogenology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/j.theriogenology.2011.09.002","article-title":"Impact of genomic selection of AI dairy sires on their likely utilization and methods to estimate fertility: A paradigm shift","volume":"77","author":"Amann","year":"2012","journal-title":"Theriogenology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"283","DOI":"10.2527\/2006.842283x","article-title":"Genetic parameter estimates for scrotal circumference and semen characteristics of Line 1 Hereford bulls","volume":"84","author":"Kealey","year":"2006","journal-title":"J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.rbmo.2010.06.022","article-title":"Transcriptome analysis of bull spermatozoa: Implications for male fertility","volume":"21","author":"Feugang","year":"2010","journal-title":"Reprod. Biomed. Online"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Selvaraju, S., Parthipan, S., Somashekar, L., Kolte, A.P., Krishnan Binsila, B., Arangasamy, A., and Ravindra, J.P. (2017). Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa. Sci. Rep., 7.","DOI":"10.1038\/srep42392"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1095\/biolreprod.106.059030","article-title":"Transcriptome analysis of bull semen with extreme nonreturn rate: Use of suppression-subtractive hybridization to identify functional markers for fertility","volume":"78","author":"Lalancette","year":"2008","journal-title":"Biol. Reprod."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1016\/j.bbagen.2016.04.001","article-title":"Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility","volume":"1860","author":"Samanta","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1177\/1933719114558918","article-title":"Sperm Proteome: What Is on the Horizon?","volume":"22","author":"Mohanty","year":"2015","journal-title":"Reprod. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5403","DOI":"10.1021\/pr501031x","article-title":"Investigation of male infertility using quantitative comparative proteomics","volume":"13","author":"Legare","year":"2014","journal-title":"J. Proteome Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1074\/mcp.M112.020123","article-title":"Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis","volume":"12","author":"Gan","year":"2013","journal-title":"Mol. Cell. Proteom."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.aju.2017.11.016","article-title":"Update on the proteomics of male infertility: A systematic review","volume":"16","author":"Agarwal","year":"2018","journal-title":"Arab J. Urol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"9656","DOI":"10.3168\/jds.2017-13288","article-title":"Predicting bull fertility using genomic data and biological information","volume":"100","author":"Morota","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5774","DOI":"10.3168\/jds.2014-8951","article-title":"Genome-wide association study for semen production traits in Holstein-Friesian bulls","volume":"98","author":"Suchocki","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"s172","DOI":"10.1017\/S1751731118000599","article-title":"Review: Genomics of bull fertility","volume":"12","author":"Taylor","year":"2018","journal-title":"Animal"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1365-2052.2012.02350.x","article-title":"Genome-wide association study identifies candidate markers for bull fertility in Holstein dairy cattle","volume":"43","author":"Penagaricano","year":"2012","journal-title":"Anim. Genet."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Han, Y., and Penagaricano, F. (2016). Unravelling the genomic architecture of bull fertility in Holstein cattle. BMC Genet., 17.","DOI":"10.1186\/s12863-016-0454-6"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1111\/j.1439-0531.2010.01648.x","article-title":"Comparative genomics approach to identify candidate genetic loci for male fertility","volume":"46","author":"Ogorevc","year":"2011","journal-title":"Reprod. Domest. Anim."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Whiston, R., Finlay, E.K., McCabe, M.S., Cormican, P., Flynn, P., Cromie, A., Hansen, P.J., Lyons, A., Fair, S., and Lonergan, P. (2017). A dual targeted beta-defensin and exome sequencing approach to identify, validate and functionally characterise genes associated with bull fertility. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-12498-x"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Pausch, H., Kolle, S., Wurmser, C., Schwarzenbacher, H., Emmerling, R., Jansen, S., Trottmann, M., Fuerst, C., Gotz, K.U., and Fries, R. (2014). A nonsense mutation in TMEM95 encoding a nondescript transmembrane protein causes idiopathic male subfertility in cattle. PLoS Genet., 10.","DOI":"10.1371\/journal.pgen.1004044"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Pausch, H., Venhoranta, H., Wurmser, C., Hakala, K., Iso-Touru, T., Sironen, A., Vingborg, R.K., Lohi, H., Soderquist, L., and Fries, R. (2016). A frameshift mutation in ARMC3 is associated with a tail stump sperm defect in Swedish Red (Bos taurus) cattle. BMC Genet., 17.","DOI":"10.1186\/s12863-016-0356-7"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1093\/biolre\/iox065","article-title":"Subfertility in bulls carrying a nonsense mutation in transmembrane protein 95 is due to failure to interact with the oocyte vestments","volume":"97","author":"Kelly","year":"2017","journal-title":"Biol. Reprod."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1111\/age.12348","article-title":"Naturally occurring genetic mutations in the 5\u2019-upstream regulatory region of bovine FSHB generate a novel cis-regulatory element that affects its expression","volume":"46","author":"Dai","year":"2015","journal-title":"Anim. Genet."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.bbalip.2013.01.002","article-title":"Mammalian alpha beta hydrolase domain (ABHD) proteins: Lipid metabolizing enzymes at the interface of cell signaling and energy metabolism","volume":"1831","author":"Lord","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1164\/rccm.201207-1280OC","article-title":"Systemic steroid exposure is associated with differential methylation in chronic obstructive pulmonary disease","volume":"186","author":"Wan","year":"2012","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1016\/j.celrep.2015.07.058","article-title":"TMC1 and TMC2 Localize at the Site of Mechanotransduction in Mammalian Inner Ear Hair Cell Stereocilia","volume":"12","author":"Kurima","year":"2015","journal-title":"Cell Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.neuron.2013.06.019","article-title":"TMC1 and TMC2 are components of the mechanotransduction channel in hair cells of the mammalian inner ear","volume":"79","author":"Pan","year":"2013","journal-title":"Neuron"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Uhlen, M., Fagerberg, L., Hallstrom, B.M., Lindskog, C., Oksvold, P., Mardinoglu, A., Sivertsson, A., Kampf, C., Sjostedt, E., and Asplund, A. (2015). Proteomics. Tissue-based map of the human proteome. Science, 347.","DOI":"10.1126\/science.1260419"},{"key":"ref_36","unstructured":"Carrell, D.T., Cairns, B., Aston, K.I., Jenkins, T., Smith, A.D., Uren, P.J., and Horsager, A. (2020, January 16). Methods of Identifying Male Fertility Status and Embryo Quality. Available online: https:\/\/patentscope.wipo.int\/search\/en\/detail.jsf?docId=WO2017024311&_cid=P22-K5CGNG-22960-1."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"131","DOI":"10.29252\/ijrm.16.3.131","article-title":"Basic and clinical genetic studies on male infertility in Iran during 2000\u20132016: A review","volume":"16","author":"Moghbelinejad","year":"2018","journal-title":"Int. J. Reprod. Biomed. (Yazd)"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1038\/s41585-018-0003-3","article-title":"Genetics of male infertility","volume":"15","author":"Krausz","year":"2018","journal-title":"Nat. Rev. Urol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.rbmo.2017.12.005","article-title":"A search for molecular mechanisms underlying male idiopathic infertility","volume":"36","author":"Bracke","year":"2018","journal-title":"Reprod. Biomed. Online"},{"key":"ref_40","first-page":"12","article-title":"Disorders of spermatogenesis: Perspectives for novel genetic diagnostics after 20 years of unchanged routine","volume":"30","author":"Tuttelmann","year":"2018","journal-title":"Med. Genet."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6122","DOI":"10.3168\/jds.2012-5591","article-title":"Comparative genomics between fly, mouse, and cattle identifies genes associated with sire conception rate","volume":"95","author":"Li","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4695","DOI":"10.3168\/jds.2010-3728","article-title":"A whole-genome association analysis of noncompensatory fertility in Holstein bulls","volume":"94","author":"Blaschek","year":"2011","journal-title":"J. Dairy Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1111\/j.2047-2927.2013.00101.x","article-title":"Genome-wide association study for inhibin, luteinizing hormone, insulin-like growth factor 1, testicular size and semen traits in bovine species","volume":"1","author":"Fortes","year":"2013","journal-title":"Andrology"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Hoglund, J.K., Sahana, G., Guldbrandtsen, B., and Lund, M.S. (2014). Validation of associations for female fertility traits in Nordic Holstein, Nordic Red and Jersey dairy cattle. BMC Genet., 15.","DOI":"10.1186\/1471-2156-15-8"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Nicolini, P., Amorin, R., Han, Y., and Penagaricano, F. (2018). Whole-genome scan reveals significant non-additive effects for sire conception rate in Holstein cattle. BMC Genet., 19.","DOI":"10.1186\/s12863-018-0600-4"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Nani, J.P., Rezende, F.M., and Penagaricano, F. (2019). Predicting male fertility in dairy cattle using markers with large effect and functional annotation data. BMC Genom., 20.","DOI":"10.1186\/s12864-019-5644-y"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3988","DOI":"10.1021\/acs.jproteome.6b00390","article-title":"Deep Coverage Proteomics Identifies More Low-Abundance Missing Proteins in Human Testis Tissue with Q-Exactive HF Mass Spectrometer","volume":"15","author":"Wei","year":"2016","journal-title":"J. Proteome Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.1093\/humrep\/des301","article-title":"Global human tissue profiling and protein network analysis reveals distinct levels of transcriptional germline-specificity and identifies target genes for male infertility","volume":"27","author":"Chalmel","year":"2012","journal-title":"Hum. Reprod."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1126\/science.aad6887","article-title":"Unconventional endocannabinoid signaling governs sperm activation via the sex hormone progesterone","volume":"352","author":"Miller","year":"2016","journal-title":"Science"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1095\/biolreprod.105.049304","article-title":"Lipid remodeling of murine epididymosomes and spermatozoa during epididymal maturation","volume":"74","author":"Rejraji","year":"2006","journal-title":"Biol. Reprod."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"d103","DOI":"10.2741\/A119","article-title":"Membrane lipid dynamics during human sperm capacitation","volume":"1","author":"Morros","year":"1996","journal-title":"Front. Biosci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0167-7306(08)60519-0","article-title":"Sphingolipids: Metabolism and cell signalling","volume":"31","author":"Merrill","year":"1996","journal-title":"New Compr. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.prostaglandins.2013.02.002","article-title":"Endogenous cannabinoids revisited: A biochemistry perspective","volume":"102","author":"Fonseca","year":"2013","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1095\/biolreprod57.5.976","article-title":"Relationship between spermatozoan lipid composition and fertility during aging of chickens","volume":"57","author":"Cerolini","year":"1997","journal-title":"Biol. Reprod."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1096\/fj.14-259382","article-title":"Imbalanced lipid homeostasis in the conditional Dicer1 knockout mouse epididymis causes instability of the sperm membrane","volume":"29","author":"Bjorkgren","year":"2015","journal-title":"FASEB J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1093\/oxfordjournals.jbchem.a021497","article-title":"Isolation and characterization of ECT1 gene encoding CTP: Phosphoethanolamine cytidylyltransferase of Saccharomyces cerevisiae","volume":"120","author":"Kawamata","year":"1996","journal-title":"J. Biochem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.cmet.2006.03.007","article-title":"The ratio of phosphatidylcholine to phosphatidylethanolamine influences membrane integrity and steatohepatitis","volume":"3","author":"Li","year":"2006","journal-title":"Cell Metab."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1046\/j.1439-0272.2002.00508.x","article-title":"Deterioration of spermatozoal plasma membrane is associated with an increase of sperm lyso-phosphatidylcholines","volume":"34","author":"Glander","year":"2002","journal-title":"Andrologia"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1007\/s11745-007-3108-7","article-title":"Characteristic oxidation products of choline plasmalogens are detectable in cattle and roe deer spermatozoa by MALDI-TOF mass spectrometry","volume":"42","author":"Fuchs","year":"2007","journal-title":"Lipids"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1038\/ng.548","article-title":"Variance component model to account for sample structure in genome-wide association studies","volume":"42","author":"Kang","year":"2010","journal-title":"Nat. Genet."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nrg3382","article-title":"The nature of confounding in genome-wide association studies","volume":"14","author":"Vilhjalmsson","year":"2013","journal-title":"Nat. Rev. Genet."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and accurate short read alignment with Burrows-Wheeler transform","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Van der Auwera, G.A., Carneiro, M.O., Hartl, C., Poplin, R., Del Angel, G., Levy-Moonshine, A., Jordan, T., Shakir, K., Roazen, D., and Thibault, J. (2013). From FastQ data to high confidence variant calls: The Genome Analysis Toolkit best practices pipeline. Curr. Protoc. Bioinform., 43.","DOI":"10.1002\/0471250953.bi1110s43"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.2144\/99276bc04","article-title":"Spreadsheet software for thermodynamic melting point prediction of oligonucleotide hybridization with and without mismatches","volume":"27","author":"Schutz","year":"1999","journal-title":"Biotechniques"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2094","DOI":"10.1093\/clinchem\/45.12.2094","article-title":"Application of a thermodynamic nearest-neighbor model to estimate nucleic acid stability and optimize probe design: Prediction of melting points of multiple mutations of apolipoprotein B-3500 and factor V with a hybridization probe genotyping assay on the LightCycler","volume":"45","author":"Oellerich","year":"1999","journal-title":"Clin. Chem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"398","DOI":"10.2527\/jas1972.352398x","article-title":"Total phospholipid and phospholipid fatty acids of ejaculated and epididymal semen and seminal vesicle fluids of boars","volume":"35","author":"Johnson","year":"1972","journal-title":"J. Anim. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s40104-017-0148-6","article-title":"Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs","volume":"8","author":"Angrimani","year":"2017","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1073\/pnas.1019267108","article-title":"Membrane lipidome of an epithelial cell line","volume":"108","author":"Sampaio","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1073\/pnas.0811700106","article-title":"Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry","volume":"106","author":"Ejsing","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1002\/ejlt.201500145","article-title":"An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of blood plasma intact lipids","volume":"117","author":"Surma","year":"2015","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Herzog, R., Schuhmann, K., Schwudke, D., Sampaio, J.L., Bornstein, S.R., Schroeder, M., and Shevchenko, A. (2012). LipidXplorer: A software for consensual cross-platform lipidomics. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0029851"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Herzog, R., Schwudke, D., Schuhmann, K., Sampaio, J.L., Bornstein, S.R., Schroeder, M., and Shevchenko, A. (2011). A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language. Genome Biol., 12.","DOI":"10.1186\/gb-2011-12-1-r8"},{"key":"ref_74","unstructured":"Zorn, C. (2004). Shapiro-Wilk test. The SAGE Encyclopedia of Social Science Research Methods, Sage Publications, Inc."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: A practical and powerful approach to multiple testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. R. Stat. Soc. B"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/2\/627\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:20:03Z","timestamp":1760361603000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/2\/627"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,17]]},"references-count":75,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["ijms21020627"],"URL":"https:\/\/doi.org\/10.3390\/ijms21020627","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,17]]}}}