{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T22:31:55Z","timestamp":1785450715895,"version":"3.56.0"},"reference-count":58,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,18]],"date-time":"2020-01-18T00:00:00Z","timestamp":1579305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The demyelinating diseases of the central nervous system involve myelin abnormalities, oligodendrocyte damage, and consequent glia activation. Neurotoxicant cuprizone (CPZ) was used to establish a mouse model of demyelination. However, the effects of CPZ on microRNA (miRNA) expression and behavior have not been clearly reported. We analyzed the behavior of mice administered a diet containing 0.2% CPZ for 6 weeks, followed by 6 weeks of recovery. Rotarod analysis demonstrated that the treated group had poorer motor coordination than control animals. This effect was reversed after 6 weeks of CPZ withdrawal. Open-field tests showed that CPZ-treated mice exhibited significantly increased anxiety and decreased exploratory behavior. CPZ-induced demyelination was observed to be alleviated after 4 weeks of CPZ treatment, according to luxol fast blue (LFB) staining and myelin basic protein (MBP) expression. miRNA expression profiling showed that the expression of 240 miRNAs was significantly changed in CPZ-fed mice compared with controls. Furthermore, miR-155-5p and miR-20a-5p upregulations enhanced NgR induction through Smad 2 and Smad 4 suppression in demyelination. Taken together, our results demonstrate that CPZ-mediated demyelination induces behavioral deficits with apparent alterations in miRNA expression, suggesting that differences in miRNA expression in vivo may be new potential therapeutic targets for remyelination.<\/jats:p>","DOI":"10.3390\/ijms21020646","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T04:27:09Z","timestamp":1579494429000},"page":"646","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Differential Expression of miRNAs and Behavioral Change in the Cuprizone-Induced Demyelination Mouse Model"],"prefix":"10.3390","volume":"21","author":[{"given":"Seung Ro","family":"Han","sequence":"first","affiliation":[{"name":"Eulji Biomedical Science Research Institute, Eulji University School of Medicine, Daejeon 34824, Korea"},{"name":"Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Hee","family":"Kang","sequence":"additional","affiliation":[{"name":"Eulji Biomedical Science Research Institute, Eulji University School of Medicine, Daejeon 34824, Korea"},{"name":"Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyungtaek","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhyuk","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Jin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae-Yong","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neuroscience, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Seek","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Min","family":"Yoo","sequence":"additional","affiliation":[{"name":"Eulji Biomedical Science Research Institute, Eulji University School of Medicine, Daejeon 34824, Korea"},{"name":"Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myung-Shin","family":"Lee","sequence":"additional","affiliation":[{"name":"Eulji Biomedical Science Research Institute, Eulji University School of Medicine, Daejeon 34824, Korea"},{"name":"Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Hoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Eulji Biomedical Science Research Institute, Eulji University School of Medicine, Daejeon 34824, Korea"},{"name":"Department of Neurosurgery, Eulji University School of Medicine, Daejeon 34824, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1016\/S0140-6736(08)61620-7","article-title":"Multiple sclerosis","volume":"372","author":"Compston","year":"2008","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.neubiorev.2019.08.008","article-title":"Behavioural phenotypes in the cuprizone model of central nervous system demyelination","volume":"107","author":"Sen","year":"2019","journal-title":"Neurosci. 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