{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T15:26:07Z","timestamp":1782314767770,"version":"3.54.5"},"reference-count":55,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T00:00:00Z","timestamp":1580256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Council of Lithuania under the initiative of Researcher groups projects","award":["MIP-007\/2014"],"award-info":[{"award-number":["MIP-007\/2014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Gastric cancer (GC) is one of the most common and lethal gastrointestinal malignancies worldwide. Many studies have shown that development of GC and other malignancies is mainly driven by alterations of cellular signaling pathways. MicroRNAs (miRNAs) are small noncoding molecules that function as tumor-suppressors or oncogenes, playing an essential role in a variety of fundamental biological processes. In order to understand the functional relevance of miRNA dysregulation, studies analyzing their target genes are of major importance. Here, we chose to analyze two miRNAs, miR-20b and miR-451a, shown to be deregulated in many different malignancies, including GC. Deregulated expression of miR-20b and miR-451a was determined in GC cell lines and the INS-GAS mouse model. Using Western Blot and luciferase reporter assay we determined that miR-20b directly regulates expression of PTEN and TXNIP, and miR-451a: CAV1 and TSC1. Loss-of-function experiments revealed that down-regulation of miR-20b and up-regulation of miR-451a expression exhibits an anti-tumor effect in vitro (miR-20b: reduced viability, colony formation, increased apoptosis rate, and miR-451a: reduced colony forming ability). To summarize, the present study identified that expression of miR-20b and miR-451a are deregulated in vitro and in vivo and have a tumor suppressive role in GC through regulation of the PI3K\/AKT\/mTOR signaling pathway.<\/jats:p>","DOI":"10.3390\/ijms21030877","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T10:51:07Z","timestamp":1580295067000},"page":"877","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["miR-20b and miR-451a Are Involved in Gastric Carcinogenesis through the PI3K\/AKT\/mTOR Signaling Pathway: Data from Gastric Cancer Patients, Cell Lines and Ins-Gas Mouse Model"],"prefix":"10.3390","volume":"21","author":[{"given":"Greta","family":"Streleckiene","sequence":"first","affiliation":[{"name":"Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT-50161, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruta","family":"Inciuraite","sequence":"additional","affiliation":[{"name":"Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT-50161, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simonas","family":"Juzenas","sequence":"additional","affiliation":[{"name":"Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT-50161, Lithuania"},{"name":"Institute of Clinical Molecular Biology, Christian-Albrechts-University of Kiel, 24105 Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violeta","family":"Salteniene","sequence":"additional","affiliation":[{"name":"Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT-50161, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruta","family":"Steponaitiene","sequence":"additional","affiliation":[{"name":"Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT-50161, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ugne","family":"Gyvyte","sequence":"additional","affiliation":[{"name":"Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT-50161, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gediminas","family":"Kiudelis","sequence":"additional","affiliation":[{"name":"Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT-50161, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcis","family":"Leja","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Preventive Medicine, University of Latvia, Riga LV-1586, Latvia"},{"name":"Faculty of Medicine, University of Latvia, Riga LV-1586, Latvia"},{"name":"Department of Research, Riga East University Hospital, Riga LV-1038, Latvia"},{"name":"Digestive Diseases Centre GASTRO, Riga LV-1079, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4501-4073","authenticated-orcid":false,"given":"Paulius","family":"Ruzgys","sequence":"additional","affiliation":[{"name":"Biophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Kaunas LT-44404, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9001-7767","authenticated-orcid":false,"given":"Saulius","family":"Satkauskas","sequence":"additional","affiliation":[{"name":"Biophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Kaunas LT-44404, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenija","family":"Kupcinskiene","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Nature Sciences, Vytautas Magnus University, Kaunas LT-44404, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Franke","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Otto-von-Guericke University, 39120 Magdeburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cosima","family":"Thon","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University, 39120 Magdeburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9514-4562","authenticated-orcid":false,"given":"Alexander","family":"Link","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, 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J. Cancer"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"312","DOI":"10.2741\/4210","article-title":"Molecular diagnostics in gastric cancer","volume":"19","author":"Bornschein","year":"2014","journal-title":"Front. Biosci. (Landmark Ed)."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-94-007-5590-1_1","article-title":"microRNAs in Human Cancer","volume":"774","author":"Farazi","year":"2013","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1038\/nrg.2016.134","article-title":"A network-biology perspective of microRNA function and dysfunction in cancer","volume":"17","author":"Bracken","year":"2016","journal-title":"Nat. Rev. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1007\/s10637-016-0407-y","article-title":"Phase I study of MRX34, a liposomal miR-34a mimic, administered twice weekly in patients with advanced solid tumors","volume":"35","author":"Beg","year":"2017","journal-title":"Invest. New Drugs"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1200\/JCO.2016.34.15_suppl.2547","article-title":"A phase I\/II study of TKM-080301, a PLK1 -targeted RNAi in patients with adrenocortical cancer (ACC)","volume":"34","author":"Demeure","year":"2016","journal-title":"J. Clin. Oncol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"11006","DOI":"10.1200\/jco.2015.33.15_suppl.11006","article-title":"Safety and activity of DCR-MYC, a first-in-class Dicer-substrate small interfering RNA (DsiRNA) targeting MYC, in a phase I study in patients with advanced solid tumors","volume":"33","author":"Tolcher","year":"2015","journal-title":"J. Clin. Oncol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1158\/1535-7163.MCT-16-0541","article-title":"Preclinical Mammalian Safety Studies of EPHARNA (DOPC Nanoliposomal EphA2-Targeted siRNA)","volume":"16","author":"Wagner","year":"2017","journal-title":"Mol. Cancer Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"19413","DOI":"10.1038\/srep19413","article-title":"Plasma extracellular RNA profiles in healthy and cancer patients","volume":"6","author":"Yuan","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.3748\/wjg.v24.i30.3313","article-title":"MicroRNAs as non-invasive diagnostic biomarkers for gastric cancer: Current insights and future perspectives","volume":"24","author":"Link","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Juzenas, S., Salteniene, V., Kupcinskas, J., Link, A., Kiudelis, G., Jonaitis, L., Jarmalaite, S., Kupcinskas, L., Malfertheiner, P., and Skieceviciene, J. (2015). Analysis of deregulated micrornas and their target genes in gastric cancer. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0135762"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kupcinskas, J., Wex, T., Link, A., Leja, M., Bruzaite, I., Steponaitiene, R., Juzenas, S., Gyvyte, U., Ivanauskas, A., and Ancans, G. (2014). Gene polymorphisms of micrornas in helicobacter pylori-induced high risk atrophic gastritis and gastric cancer. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087467"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.2147\/OTT.S85236","article-title":"miR-20b overexpression is predictive of poor prognosis in gastric cancer","volume":"8","author":"Xue","year":"2015","journal-title":"Onco. Targets. Ther."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1111\/j.1440-1746.2008.05666.x","article-title":"Differential expression of microRNA species in human gastric cancer versus non-tumorous tissues","volume":"24","author":"Guo","year":"2009","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1002\/ijc.28850","article-title":"Genetic association of gastric cancer with miRNA clusters including the cancer-related genes MIR29, MIR25, MIR93 and MIR106: Results from the EPIC-EURGAST study","volume":"135","author":"Bonet","year":"2014","journal-title":"Int. J. Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s13402-015-0247-3","article-title":"miR-101-2, miR-125b-2 and miR-451a act as potential tumor suppressors in gastric cancer through regulation of the PI3K\/AKT\/mTOR pathway","volume":"39","author":"Riquelme","year":"2016","journal-title":"Cell. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kiyosawa, N., Watanabe, K., Toyama, K., and Ishizuka, H. (2019). Circulating miRNA Signature as a Potential Biomarker for the Prediction of Analgesic Efficacy of Hydromorphone. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20071665"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1158\/1535-7163.MCT-12-0802","article-title":"The Potential Role of miR-451 in Cancer Diagnosis, Prognosis, and Therapy","volume":"12","author":"Pan","year":"2013","journal-title":"Mol. Cancer Ther."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wang, B., Yang, J., and Xiao, B. (2016). MicroRNA-20b (miR-20b) Promotes the Proliferation, Migration, Invasion, and Tumorigenicity in Esophageal Cancer Cells via the Regulation of Phosphatase and Tensin Homologue Expression. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0164105"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.3892\/ijo.2017.4155","article-title":"MicroRNA-20b promotes cell proliferation via targeting of TGF-\u03b2 receptor II and upregulates MYC expression in Ewing\u2019s sarcoma cells","volume":"51","author":"Kawano","year":"2017","journal-title":"Int. J. Oncol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ulivi, P., Canale, M., Passardi, A., Marisi, G., Valgiusti, M., Frassineti, G.L., Calistri, D., Amadori, D., and Scarpi, E. (2018). Circulating plasma levels of miR-20b, miR-29b and mir-155 as predictors of bevacizumab efficacy in patients with metastatic colorectal cancer. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19010307"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.jmb.2004.03.065","article-title":"Molecular Evolution of a MicroRNA Cluster","volume":"339","author":"Tanzer","year":"2004","journal-title":"J. Mol. Biol."},{"key":"ref_23","first-page":"6895","article-title":"miR-20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer","volume":"14","author":"Guo","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"12188","DOI":"10.18632\/oncotarget.3664","article-title":"miR-20b is up-regulated in brain metastases from primary breast cancers","volume":"6","author":"Ahmad","year":"2015","journal-title":"Oncotarget"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.4161\/cc.21278","article-title":"Dysregulated microRNAs in the pathogenesis and progression of cervical neoplasm","volume":"11","author":"Cheung","year":"2012","journal-title":"Cell Cycle"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.humimm.2014.01.006","article-title":"MiR-20b, -21, and -130b inhibit PTEN expression resulting in B7-H1 over-expression in advanced colorectal cancer","volume":"75","author":"Zhu","year":"2014","journal-title":"Hum. Immunol."},{"key":"ref_27","first-page":"537","article-title":"microRNA expression profile in undifferentiated gastric cancer","volume":"34","author":"Ishiguro","year":"1992","journal-title":"Int. J. Oncol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/S1470-2045(09)70343-2","article-title":"Relation between microRNA expression and progression and prognosis of gastric cancer: A microRNA expression analysis","volume":"11","author":"Ueda","year":"2010","journal-title":"Lancet Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1016\/S0016-5085(03)00406-2","article-title":"Host and Microbial Constituents Influence Helicobacter pylori-Induced Cancer in a Murine Model of Hypergastrinemia","volume":"124","author":"Fox","year":"2003","journal-title":"Gastroenterology."},{"key":"ref_30","first-page":"471","article-title":"Stomach cancer incidence in the world","volume":"31","author":"Yamamoto","year":"2001","journal-title":"Jpn J. Clin. Oncol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/2045-3701-4-62","article-title":"MicroRNA-20b promotes cell growth of breast cancer cells partly via targeting phosphatase and tensin homologue (PTEN)","volume":"4","author":"Zhou","year":"2014","journal-title":"Cell Biosci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"12731","DOI":"10.18632\/oncotarget.7262","article-title":"miR-451a is underexpressed and targets AKT\/mTOR pathway in papillary thyroid carcinoma","volume":"7","author":"Minna","year":"2016","journal-title":"Oncotarget"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1002\/mgg3.473","article-title":"MicroRNA-451a acts as tumor suppressor in cutaneous basal cell carcinoma","volume":"6","author":"Sun","year":"2018","journal-title":"Mol. Genet. Genomic Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1111\/cas.13526","article-title":"Impact of novel oncogenic pathways regulated by antitumor miR-451a in renal cell carcinoma","volume":"109","author":"Yamada","year":"2018","journal-title":"Cancer Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1007\/s12094-016-1516-y","article-title":"Identification and functional characterization of microRNAs reveal a potential role in gastric cancer progression","volume":"19","author":"Li","year":"2017","journal-title":"Clin. Transl. Oncol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s00428-014-1588-4","article-title":"The PI3K\/AKT\/mTOR pathway is activated in gastric cancer with potential prognostic and predictive significance","volume":"465","author":"Tapia","year":"2014","journal-title":"Virchows Arch."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1002\/ijc.26396","article-title":"mTOR as a therapeutic target in patients with gastric cancer","volume":"130","author":"Ducreux","year":"2012","journal-title":"Int. J. Cancer"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"187","DOI":"10.2174\/156800908784293659","article-title":"The PTEN\/PI3K\/AKT Signalling Pathway in Cancer, Therapeutic Implications","volume":"8","author":"Carnero","year":"2008","journal-title":"Curr. Cancer Drug Targets"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1101\/gad.1777409","article-title":"The PTEN-regulating microRNA miR-26a is amplified in high-grade glioma and facilitates gliomagenesis in vivo","volume":"23","author":"Huse","year":"2009","journal-title":"Genes Dev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1476-4598-12-30","article-title":"MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and promotes growth, migration, and invasion in colorectal carcinoma cells","volume":"12","author":"Wu","year":"2013","journal-title":"Mol. Cancer"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1158\/0008-5472.CAN-07-2488","article-title":"MicroRNA Expression Profiling in Human Ovarian Cancer: miR-214 Induces Cell Survival and Cisplatin Resistance by Targeting PTEN","volume":"68","author":"Yang","year":"2008","journal-title":"Cancer Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.1016\/j.biocel.2011.09.005","article-title":"TXNIP (VDUP-1, TBP-2): A major redox regulator commonly suppressed in cancer by epigenetic mechanisms","volume":"43","author":"Zhou","year":"2011","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.mito.2012.06.004","article-title":"Roles of thioredoxin binding protein (TXNIP) in oxidative stress, apoptosis and cancer","volume":"13","author":"Zhou","year":"2013","journal-title":"Mitochondrion"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"514","DOI":"10.3389\/fimmu.2013.00514","article-title":"Thioredoxin\/Txnip: Redoxisome, as a Redox Switch for the Pathogenesis of Diseases","volume":"4","author":"Yoshihara","year":"2014","journal-title":"Front. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.cellsig.2016.01.011","article-title":"Oncogenic activation of the PI3K\/Akt pathway promotes cellular glucose uptake by downregulating the expression of thioredoxin-interacting protein","volume":"28","author":"Hong","year":"2016","journal-title":"Cell. Signal."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"32701","DOI":"10.18632\/oncotarget.4702","article-title":"MiR-373 drives the epithelial-to-mesenchymal transition and metastasis via the miR-373-TXNIP-HIF1\u03b1-TWIST signaling axis in breast cancer","volume":"6","author":"Chen","year":"2015","journal-title":"Oncotarget"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1038\/s41388-018-0534-3","article-title":"Oncogenic microRNA-411 promotes lung carcinogenesis by directly targeting suppressor genes SPRY4 and TXNIP","volume":"38","author":"Zhang","year":"2019","journal-title":"Oncogene"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1159\/000492309","article-title":"MicroRNA-224 Promotes Pancreatic Cancer Cell Proliferation and Migration by Targeting the TXNIP-Mediated HIF1\u03b1 Pathway","volume":"48","author":"Zhu","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.15252\/embr.201439392","article-title":"E2F1 induces miR-224\/452 expression to drive EMT through TXNIP downregulation","volume":"15","author":"Knoll","year":"2014","journal-title":"EMBO Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"C935","DOI":"10.1152\/ajpcell.00121.2009","article-title":"Caveolin-1 plays important role in EGF-induced migration and proliferation of mouse embryonic stem cells: involvement of PI3K\/Akt and ERK","volume":"297","author":"Park","year":"2009","journal-title":"Am. J. Physiol. Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1016\/S0006-291X(02)00928-2","article-title":"Some protein tyrosine phosphatases target in part to lipid rafts and interact with caveolin-1","volume":"296","author":"Caselli","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_52","first-page":"2811","article-title":"MicroRNA-124 inhibits cell proliferation, invasion and migration by targeting CAV1 in bladder cancer","volume":"16","author":"Zhou","year":"2018","journal-title":"Exp. Ther. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s13048-019-0533-1","article-title":"miR-96-5p promotes the proliferation and migration of ovarian cancer cells by suppressing Caveolae1","volume":"12","author":"Liu","year":"2019","journal-title":"J. Ovarian Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.ebiom.2018.11.004","article-title":"Exosomal miRNA confers chemo resistance via targeting Cav1\/p-gp\/M2-type macrophage axis in ovarian cancer","volume":"38","author":"Kanlikilicer","year":"2018","journal-title":"EBioMedicine"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1038\/nprot.2010.43","article-title":"Detection of Mycoplasma in cell cultures","volume":"5","author":"Young","year":"2010","journal-title":"Nat. Protoc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/3\/877\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:53:05Z","timestamp":1760172785000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/3\/877"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,29]]},"references-count":55,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["ijms21030877"],"URL":"https:\/\/doi.org\/10.3390\/ijms21030877","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,29]]}}}