{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:37:21Z","timestamp":1791995841644,"version":"4.4.0"},"reference-count":38,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T00:00:00Z","timestamp":1580428800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002426","name":"Fondazione Telethon","doi-asserted-by":"publisher","award":["GGP13233"],"award-info":[{"award-number":["GGP13233"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Duchenne muscular dystrophy (DMD) is a genetic disease characterized by muscle wasting and chronic inflammation, leading to impaired satellite cells (SCs) function and exhaustion of their regenerative capacity. We previously showed that lack of PKC\u03b8 in mdx mice, a mouse model of DMD, reduces muscle wasting and inflammation, and improves muscle regeneration and performance at early stages of the disease. In this study, we show that muscle regeneration is boosted, and fibrosis reduced in mdx\u03b8\u2212\/\u2212 mice, even at advanced stages of the disease. This phenotype was associated with a higher number of Pax7 positive cells in mdx\u03b8\u2212\/\u2212 muscle compared with mdx muscle, during the progression of the disease. Moreover, the expression level of Pax7 and Notch1, the pivotal regulators of SCs self-renewal, were upregulated in SCs isolated from mdx\u03b8\u2212\/\u2212 muscle compared with mdx derived SCs. Likewise, the expression of the Notch ligands Delta1 and Jagged1 was higher in mdx\u03b8\u2212\/\u2212 muscle compared with mdx. The expression level of Delta1 and Jagged1 was also higher in PKC\u03b8\u2212\/\u2212 muscle compared with WT muscle following acute injury. In addition, lack of PKC\u03b8 prolonged the survival and sustained the differentiation of transplanted myogenic progenitors. Overall, our results suggest that lack of PKC\u03b8 promotes muscle repair in dystrophic mice, supporting stem cells survival and maintenance through increased Delta-Notch signaling.<\/jats:p>","DOI":"10.3390\/ijms21030932","type":"journal-article","created":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T05:55:46Z","timestamp":1580450146000},"page":"932","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Lack of PKC\u03b8 Promotes Regenerative Ability of Muscle Stem Cells in Chronic Muscle Injury"],"prefix":"10.3390","volume":"21","author":[{"given":"Piera Filomena","family":"Fiore","sequence":"first","affiliation":[{"name":"Department of AHFMO, University of Rome \u201cla Sapienza\u201d, Via A. Scarpa 14, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Benedetti","sequence":"additional","affiliation":[{"name":"Department of AHFMO, University of Rome \u201cla Sapienza\u201d, Via A. Scarpa 14, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Sandon\u00e0","sequence":"additional","affiliation":[{"name":"Department of AHFMO, University of Rome \u201cla Sapienza\u201d, Via A. Scarpa 14, 00161 Rome, Italy"},{"name":"IRCCS Fondazione Santa Lucia (FSL), e00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Madaro","sequence":"additional","affiliation":[{"name":"Department of AHFMO, University of Rome \u201cla Sapienza\u201d, Via A. Scarpa 14, 00161 Rome, Italy"},{"name":"IRCCS Fondazione Santa Lucia (FSL), e00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8217-970X","authenticated-orcid":false,"given":"Marco","family":"De Bardi","sequence":"additional","affiliation":[{"name":"IRCCS Fondazione Santa Lucia (FSL), e00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Saccone","sequence":"additional","affiliation":[{"name":"IRCCS Fondazione Santa Lucia (FSL), e00143 Rome, Italy"},{"name":"Department of Life Sciences and Public Health, Universit\u00e0 Cattolica del Sacro Cuore, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pier Lorenzo","family":"Puri","sequence":"additional","affiliation":[{"name":"Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1742-3969","authenticated-orcid":false,"given":"Cesare","family":"Gargioli","sequence":"additional","affiliation":[{"name":"Department of Biology, Tor Vergata University, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biliana","family":"Lozanoska-Ochser","sequence":"additional","affiliation":[{"name":"Department of AHFMO, University of Rome \u201cla Sapienza\u201d, Via A. Scarpa 14, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-5360","authenticated-orcid":false,"given":"Marina","family":"Bouch\u00e9","sequence":"additional","affiliation":[{"name":"Department of AHFMO, University of Rome \u201cla Sapienza\u201d, Via A. Scarpa 14, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1091\/mbc.e06-08-0693","article-title":"Muscle satellite cells and endothelial cells: Close neighbors and privileged partners","volume":"18","author":"Christov","year":"2007","journal-title":"Mol. Biol. 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