{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T18:29:37Z","timestamp":1774376977507,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,2,5]],"date-time":"2020-02-05T00:00:00Z","timestamp":1580860800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Agency for Academic Exchange, The Bekker Programme,","award":["PPN\/BEK\/2018\/1\/00346"],"award-info":[{"award-number":["PPN\/BEK\/2018\/1\/00346"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>\u03b1-Lipoic acid, glutathione, cysteine, and cysteinylglycine can be applied as therapeutic agents in civilization diseases such as diabetes mellitus, cardiovascular diseases, and cancers. On the other hand, a higher concentration of homocysteine can result in health problems and has been indicated as an independent risk factor for cardiovascular disease and accelerated atherosclerosis. Here, the first simplified HPLC-UV assay that enables simultaneous determination of \u03b1-lipoic acid and low-molecular-mass thiols in plasma, reduces the number of steps, shortens the total time of sample preparation, and limits the amount of single-use polypropylene laboratory materials is described. The assay is based on reversed-phase high performance liquid chromatography with UV detection and simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide. Linearity in the detector responses for plasma samples were observed in ranges: 0.12\u20135.0 nmol mL\u22121 for \u03b1-lipoic acid; 2.0\u201320.0 nmol mL\u22121 for glutathione, cysteinylglycine, and homocysteine; and 40.0\u2013400.0 for cysteine. The LODs for \u03b1-lipoic acid and low-molecular-mass thiols were 0.08 and 0.12 nmol mL\u22121, respectively, while LOQs were 0.12 and 0.16 nmol mL\u22121, respectively. The usefulness of the proposed method has been proven by its application to real samples.<\/jats:p>","DOI":"10.3390\/ijms21031049","type":"journal-article","created":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T02:59:18Z","timestamp":1580957958000},"page":"1049","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A Simplified Method for Simultaneous Determination of \u03b1-Lipoic Acid and Low-Molecular-Mass Thiols in Human Plasma"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8737-7913","authenticated-orcid":false,"given":"Kamila","family":"Borowczyk","sequence":"first","affiliation":[{"name":"Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 \u0141\u00f3d\u017a, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrycja","family":"Olejarz","sequence":"additional","affiliation":[{"name":"Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 \u0141\u00f3d\u017a, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9247-5131","authenticated-orcid":false,"given":"Gra\u017cyna","family":"Chwatko","sequence":"additional","affiliation":[{"name":"Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 \u0141\u00f3d\u017a, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"Szylberg","sequence":"additional","affiliation":[{"name":"Rehabilitation Center \u201cKraszewski\u201d, Kraszewskiego 7\/9, 93-161 \u0141\u00f3d\u017a, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafa\u0142","family":"G\u0142owacki","sequence":"additional","affiliation":[{"name":"Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 \u0141\u00f3d\u017a, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.clnesp.2019.03.015","article-title":"Alpha-lipoic acid (ALA) supplementation effect on glycemic and inflammatory biomarkers: A Systematic Review and meta-analysis","volume":"32","author":"Rahimlou","year":"2019","journal-title":"Clin. 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