{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T22:11:36Z","timestamp":1784585496408,"version":"3.55.0"},"reference-count":86,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T00:00:00Z","timestamp":1581638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008781","name":"Pomeranian Medical University","doi-asserted-by":"publisher","award":["2019"],"award-info":[{"award-number":["2019"]}],"id":[{"id":"10.13039\/501100008781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008781","name":"Pomeranian Medical University","doi-asserted-by":"publisher","award":["2019"],"award-info":[{"award-number":["2019"]}],"id":[{"id":"10.13039\/501100008781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Objectives: Premature birth, defined as less than 37 weeks gestation, affects approximately 12% of all live births around the world. Advances in neonatal care have resulted in the increased survival of infants born prematurely. Although prematurity is a known risk factor for different cardiovascular diseases, little is known about the pathophysiology of vasculature during premature gestation and angiopoietic factors network during premature birth. Aims: The objective of this study was to determine whether the profile of several pro-angiogenic and anti-angiogenic factors in umbilical cord blood (UCB) is different in healthy appropriate-for-gestational-age preterm newborns and normal term babies. The second aim of this study was to investigate the microRNA (miRNAs) expression profile in UCB from preterm labor and to detect miRNAs potentially taking part in control of angogenesis-related processes (Angio-MiRs). Methods: Using an immunobead Luminex assay, we simultaneously measured the concentration of Angiogenin, Angiopoietin-1, FGF-acidic, FGF-basic, PDGF-aa, PlGF, VEGF, VEGF-D, Endostatin, Thrombospondin-2, NGF, BDNF, GDNF, and NT-4 in UCB samples collected from the preterm (n = 27) and term (n = 52) delivery. In addition, the global microRNA expression in peripheral blood mononuclear cells (PBMCs) circulating in such UCB samples was examined in this study using microarray MiRNA technique. Results: The concentrations of five from eight measured pro-angiogenic factors (VEGF, Angiopoietin-1, PDGF-AA, FGF-a, and FGF-b) were significantly lower in UCB from preterm newborns. On the contrary, two angiostatic factors (Endostatin and Thrombospondin-2) were significantly up-regulated in preterm UCB. Among analyzed neurotrophins in preterm newborns, the elevated UCB concentration was found only in the case of GDNF, whereas BDNF was significantly reduced. Moreover, two angiopoietic factors, VEGF-D and PlGF, and two neurotrophins, NT4 and NGF, did not differ in concentration in preterm and term babies. We also discovered that among the significantly down-regulated miRNAs, there were several classical Angio-MiRs (inter alia MiR-125, MiR-126, MiR-145, MiR-150, or MiR155), which are involved in angiogenesis regulation in newborn after preterm delivery. Conclusions: This is the first report of simultaneous measurements of several angiopoietic factors in UCB collected from infants during preterm and term labor. Here, we observed that several pro-angiogenic factors were at lower concentration in UCB collected from preterm newborns than term babies. In contrast, the two measured angiostatic factors, Endostatin and Thrombospondin-2, were significantly higher in UCB from preterm babies. This can suggest that distinct pathophysiological contributions from differentially expressed various angiopoietic factors may determine the clinical outcomes after preterm birth. Especially, our angiogenesis-related molecules analysis indicates that preterm birth of healthy, appropriate-for-gestational-age newborns is an \u201canti-angiogenic state\u201d that may provide an increased risk for improper development and function of cardiovascular system in the adulthood. This work also contributes to a better understanding of the role of miRNAs potentially involved in angiogenesis control in preterm newborns.<\/jats:p>","DOI":"10.3390\/ijms21041305","type":"journal-article","created":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T04:06:01Z","timestamp":1582085161000},"page":"1305","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Differential Secretion of Angiopoietic Factors and Expression of MicroRNA in Umbilical Cord Blood from Healthy Appropriate-For-Gestational-Age Preterm and Term Newborns\u2014in Search of Biomarkers of Angiogenesis-Related Processes in Preterm Birth"],"prefix":"10.3390","volume":"21","author":[{"given":"Dorota","family":"Gr\u00f3decka-Szwajkiewicz","sequence":"first","affiliation":[{"name":"Department of General Pathology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0451-8723","authenticated-orcid":false,"given":"Zofia","family":"Ula\u0144czyk","sequence":"additional","affiliation":[{"name":"Department of General Pathology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edyta","family":"Zagrodnik","sequence":"additional","affiliation":[{"name":"Department of General Pathology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4008-7473","authenticated-orcid":false,"given":"Karolina","family":"\u0141uczkowska","sequence":"additional","affiliation":[{"name":"Department of General Pathology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8884-837X","authenticated-orcid":false,"given":"Dorota","family":"Rogi\u0144ska","sequence":"additional","affiliation":[{"name":"Department of General Pathology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi\u0142osz P.","family":"Kawa","sequence":"additional","affiliation":[{"name":"Department of General Pathology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Stecewicz","sequence":"additional","affiliation":[{"name":"Department of General Pathology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9415-2758","authenticated-orcid":false,"given":"Krzysztof","family":"Safranow","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogus\u0142aw","family":"Machali\u0144ski","sequence":"additional","affiliation":[{"name":"Department of General Pathology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1542\/peds.2012-3769","article-title":"Annual summary of vital statistics: 2010\u20132011","volume":"131","author":"Hamilton","year":"2013","journal-title":"Pediatrics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1056\/NEJMoa0706475","article-title":"Long-term medical and social consequences of preterm birth","volume":"359","author":"Moster","year":"2008","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1111\/aogs.12880","article-title":"The effects of preterm birth and its antecedents on the cardiovascular system","volume":"95","author":"Bensley","year":"2016","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1161\/CIRCULATIONAHA.112.126920","article-title":"Preterm heart in adult life: Cardiovascular magnetic resonance reveals distinct differences in left ventricular mass, geometry, and function","volume":"127","author":"Lewandowski","year":"2013","journal-title":"Circulation"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1111\/apa.13080","article-title":"Adolescents who were born extremely preterm demonstrate modest decreases in exercise capacity","volume":"104","author":"Clemm","year":"2015","journal-title":"Acta Paediatr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s10654-014-9892-5","article-title":"Cerebrovascular, and ischemic heart disease in young adults born preterm: A population-based Swedish cohort study","volume":"29","author":"Ueda","year":"2014","journal-title":"Eur. J. Epidemiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.1016\/j.jacc.2017.03.572","article-title":"Preterm Birth and Risk of Heart Failure Up to Early Adulthood","volume":"69","author":"Carr","year":"2017","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1002\/ppul.23633","article-title":"Risk factors for pulmonary arterial hypertension in children and young adults","volume":"52","author":"Naumburg","year":"2017","journal-title":"Pediatr. Pulmonol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e1240","DOI":"10.1542\/peds.2012-2177","article-title":"Preterm birth and the metabolic syndrome in adult life: A systematic review and meta-analysis","volume":"131","author":"Parkinson","year":"2013","journal-title":"Pediatrics"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1161\/HYPERTENSIONAHA.111.181784","article-title":"Systematic review and meta-analysis of preterm birth and later systolic blood pressure","volume":"59","author":"Monuteaux","year":"2012","journal-title":"Hypertension"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1503\/cmaj.120143","article-title":"Pregnancy complications among women born preterm","volume":"184","author":"Boivin","year":"2012","journal-title":"CMAJ Can. Med. Assoc. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1111\/ppe.12219","article-title":"Coronary Heart Disease and Stroke in Adults Born Preterm\u2014The Helsinki Birth Cohort Study","volume":"29","author":"Kajantie","year":"2015","journal-title":"Paediatr. Perinat. Epidemiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1681\/ASN.2005030300","article-title":"Prenatal programming of hypertension: Lessons from experimental models","volume":"16","author":"Vehaskari","year":"2005","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jpeds.2018.09.058","article-title":"Obesity is Associated with Higher Blood Pressure and Higher Levels of Angiotensin II but Lower Angiotensin-(1-7) in Adolescents Born Preterm","volume":"205","author":"South","year":"2019","journal-title":"J. Pediatr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1161\/CIRCULATIONAHA.107.763730","article-title":"Impaired tissue perfusion: A pathology common to hypertension, obesity, and diabetes mellitus","volume":"118","author":"Levy","year":"2008","journal-title":"Circulation"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/s11906-010-0114-6","article-title":"Hypertension management and microvascular insulin resistance in diabetes","volume":"12","author":"Ko","year":"2010","journal-title":"Curr. Hypertens. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1161\/HYPERTENSIONAHA.114.04662","article-title":"Elevated blood pressure in preterm-born offspring associates with a distinct antiangiogenic state and microvascular abnormalities in adult life","volume":"65","author":"Lewandowski","year":"2015","journal-title":"Hypertension"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.cmet.2013.08.001","article-title":"Role of endothelial cell metabolism in vessel sprouting","volume":"18","author":"Georgiadou","year":"2013","journal-title":"Cell Metab."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1515\/jpm-2011-0199","article-title":"Circulating endothelial progenitor cells in premature infants: Is there an association with premature birth complications?","volume":"40","author":"Safranow","year":"2012","journal-title":"Perinat. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1001\/archophthalmol.2010.221","article-title":"Circulating stem cell populations in preterm infants: Implications for the development of retinopathy of prematurity","volume":"128","author":"Machalinska","year":"2010","journal-title":"Arch. Ophthalmol."},{"key":"ref_21","first-page":"302","article-title":"Evaluation of VEGF and IGF-1 plasma levels in preterm infants--potential correlation with retinopathy of prematurity, clinical implications","volume":"111","author":"Modrzejewska","year":"2009","journal-title":"Klin. Ocz."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1182\/blood-2010-12-325142","article-title":"A switch toward angiostatic gene expression impairs the angiogenic properties of endothelial progenitor cells in low birth weight preterm infants","volume":"118","author":"Ligi","year":"2011","journal-title":"Blood"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2116","DOI":"10.1182\/blood-2013-02-484956","article-title":"Accelerated senescence of cord blood endothelial progenitor cells in premature neonates is driven by SIRT1 decreased expression","volume":"123","author":"Vassallo","year":"2014","journal-title":"Blood"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7","DOI":"10.5966\/sctm.2016-0085","article-title":"Endothelial Progenitor Cells as Prognostic Markers of Preterm Birth-Associated Complications","volume":"6","author":"Bertagnolli","year":"2017","journal-title":"Stem. Cells Transl. Med."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Churov, A., Summerhill, V., Grechko, A., and Orekhova, V. (2019). Orekhov A MicroRNAs as Potential Biomarkers in Atherosclerosis. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20225547"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1161\/CIRCRESAHA.116.308434","article-title":"Circulating Noncoding RNAs as Biomarkers of Cardiovascular Disease and Injury","volume":"120","author":"Viereck","year":"2017","journal-title":"Circ. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"286","DOI":"10.3389\/fnagi.2019.00286","article-title":"The Interplay between Systemic Inflammatory Factors and MicroRNAs in Age-Related Macular Degeneration","volume":"11","author":"Grabowicz","year":"2019","journal-title":"Front. Aging Neurosci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ula\u0144czyk, Z., Sobu\u015b, A., \u0141uczkowska, K., Grabowicz, A., Mozolewska-Piotrowska, K., Safranow, K., Kawa, M.P., Pa\u0142ucha, A., Krawczyk, M., and Sikora, P. (2019). Associations of microRNAs, Angiogenesis-Regulating Factors and CFH Y402H Polymorphism-An Attempt to Search for Systemic Biomarkers in Age-Related Macular Degeneration. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20225750"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.jpeds.2015.04.060","article-title":"Downregulation of Umbilical Cord Blood Levels of miR-374a in Neonatal Hypoxic Ischemic Encephalopathy","volume":"167","author":"Looney","year":"2015","journal-title":"J. Pediatr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1001\/jamaneurol.2018.4182","article-title":"Validation of Altered Umbilical Cord Blood MicroRNA Expression in Neonatal Hypoxic-Ischemic Encephalopathy","volume":"76","author":"Looney","year":"2019","journal-title":"JAMA Neurol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1210\/er.2003-0027","article-title":"Vascular endothelial growth factor: Basic science and clinical progress","volume":"25","author":"Ferrara","year":"2004","journal-title":"Endocr. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.yexcr.2005.11.008","article-title":"VEGF function in vascular pathogenesis","volume":"312","author":"Ng","year":"2006","journal-title":"Exp. Cell Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1042\/CS20040370","article-title":"The vascular endothelial growth factor (VEGF)\/VEGF receptor system and its role under physiological and pathological conditions","volume":"109","author":"Takahashi","year":"2005","journal-title":"Clin. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1038\/nm0603-653","article-title":"Angiogenesis in health and disease","volume":"9","author":"Carmeliet","year":"2003","journal-title":"Nat. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1111\/j.1745-7270.2008.00442.x","article-title":"Mechanisms of action of angiogenin","volume":"40","author":"Gao","year":"2008","journal-title":"Acta Biochim. Biophys. Sin."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.mvr.2004.06.002","article-title":"Angiogenic synergy of bFGF and VEGF is antagonized by angiopoietin-2 in a modified in vivo Matrigel assay","volume":"68","author":"Ley","year":"2004","journal-title":"Microvasc. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s00441-003-0776-3","article-title":"VEGF and PlGF: Two pleiotropic growth factors with distinct roles in development and homeostasis","volume":"314","author":"Tjwa","year":"2003","journal-title":"Cell Tissue Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"572","DOI":"10.3390\/ph3030572","article-title":"Platelet-derived growth factor (PDGF)\/PDGF receptors (PDGFR) axis as target for antitumor and antiangiogenic therapy","volume":"3","author":"Raica","year":"2010","journal-title":"Pharmaceuticals"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1073\/pnas.91.5.1677","article-title":"Nuclear translocation of angiogenin in proliferating endothelial cells is essential to its angiogenic activity","volume":"91","author":"Moroianu","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3282","DOI":"10.1021\/bi902122w","article-title":"Angiogenin induces nitric oxide synthesis in endothelial cells through PI-3 and Akt kinases","volume":"49","author":"Trouillon","year":"2010","journal-title":"Biochemistry"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1159\/000010238","article-title":"Maternal and fetal circulating angiogenin levels in pregnancies with small-for-gestational-Age and appropriate-for-gestational-Age infants","volume":"49","author":"Yamashiro","year":"2000","journal-title":"Gynecol. Obstet. Investig."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1177\/0003319711410308","article-title":"Angiogenin and hemoxygenase in pregnancy: Influence of hypertension","volume":"63","author":"Karthikeyan","year":"2012","journal-title":"Angiology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.placenta.2012.02.013","article-title":"Placental expression of VEGF family mRNA in adverse pregnancy outcomes","volume":"33","author":"Andraweera","year":"2012","journal-title":"Placenta"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.jpeds.2017.01.015","article-title":"Cord Blood Biomarkers of Placental Maternal Vascular Underperfusion Predict Bronchopulmonary Dysplasia-Associated Pulmonary Hypertension","volume":"185","author":"Mestan","year":"2017","journal-title":"J. Pediatr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1101\/gad.13.9.1055","article-title":"Growth factors acting via endothelial cell-specific receptor tyrosine kinases: VEGFs, angiopoietins, and ephrins in vascular development","volume":"13","author":"Gale","year":"1999","journal-title":"Genes Dev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1159\/000073636","article-title":"Vascular endothelial growth factor and angiogenin levels during fetal development and in maternal diabetes","volume":"84","author":"Lassus","year":"2003","journal-title":"Biol. Neonate"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.3109\/14767050902994838","article-title":"A subset of patients destined to develop spontaneous preterm labor has an abnormal angiogenic\/anti-angiogenic profile in maternal plasma: Evidence in support of pathophysiologic heterogeneity of preterm labor derived from a longitudinal study","volume":"22","author":"Chaiworapongsa","year":"2009","journal-title":"J. Matern. Fetal. Neonatal. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1387\/ijdb.103214ec","article-title":"The role of angiogenic growth factors in organogenesis","volume":"55","author":"Crivellato","year":"2011","journal-title":"Int. J. Dev. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1042\/CS20160129","article-title":"Angiopoietin-Tie signalling in the cardiovascular and lymphatic systems","volume":"131","author":"Eklund","year":"2017","journal-title":"Clin. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/S0092-8674(00)81813-9","article-title":"Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis","volume":"87","author":"Suri","year":"1996","journal-title":"Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"4213","DOI":"10.1210\/jc.2002-020195","article-title":"Human placental vascular development: Vasculogenic and angiogenic (branching and nonbranching) transformation is regulated by vascular endothelial growth factor-A, angiopoietin-1, and angiopoietin-2","volume":"87","author":"Geva","year":"2002","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1053\/beog.2000.0138","article-title":"Angiogenesis and placental growth in normal and compromised pregnancies","volume":"14","author":"Ong","year":"2000","journal-title":"Baillieres Best Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1111\/j.1471-0528.2012.03311.x","article-title":"Accuracy of circulating placental growth factor, vascular endothelial growth factor, soluble fms-like tyrosine kinase 1 and soluble endoglin in the prediction of pre-eclampsia: A systematic review and meta-analysis.; EBM CONNECT Collaboration","volume":"119","author":"Kleinrouweler","year":"2012","journal-title":"BJOG"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1053\/beog.2000.0139","article-title":"Angiogenesis and intrauterine growth restriction","volume":"14","author":"Ahmed","year":"2000","journal-title":"Baillieres Best Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1080\/14767050802034545","article-title":"The change in concentrations of angiogenic and anti-angiogenic factors in maternal plasma between the first and second trimesters in risk assessment for the subsequent development of preeclampsia and small-for-gestational age","volume":"21","author":"Erez","year":"2008","journal-title":"J. Matern. Fetal. Neonatal. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.3109\/14767051003681121","article-title":"An imbalance between angiogenic and anti-angiogenic factors precedes fetal death in a subset of patients: Results of a longitudinal study","volume":"23","author":"Romero","year":"2010","journal-title":"J. Matern. Fetal. Neonatal. Med."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.ajog.2008.02.016","article-title":"Twin-to-twin transfusion syndrome: An antiangiogenic state?","volume":"198","author":"Kusanovic","year":"2008","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.ajog.2007.08.052","article-title":"Circulatory soluble endoglin and its predictive value for preeclampsia in second-trimester pregnancies with abnormal uterine perfusion","volume":"198","author":"Stepan","year":"2008","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1542\/peds.2008-1339","article-title":"Endostatin concentration in cord plasma predicts the development of bronchopulmonary dysplasia in very low birth weight infants","volume":"123","author":"Andersson","year":"2009","journal-title":"Pediatrics"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.yjmcc.2017.07.007","article-title":"BDNF\u2014A key player in cardiovascular system","volume":"110","year":"2017","journal-title":"J. Mol. Cell Cardiol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Baumert, B., Przybycie\u0144, K., Paczkowska, E., Kotowski, M., Pius-Sadowska, E., Safranow, K., Peregud-Pogorzelski, J., Kornacewicz-Jach, Z., Peregud-Pogorzelska, M., and Machali\u0144ski, B. (2019). Novel Evidence of the Increase in Angiogenic Factor Plasma Levels after Lineage-Negative Stem\/Progenitor Cell Intracoronary Infusion in Patients with Acute Myocardial Infarction. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20133330"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1203\/01.PDR.0000061588.39652.26","article-title":"Changes in neurotrophin levels in umbilical cord blood from infants with different gestational ages and clinical conditions","volume":"53","author":"Chouthai","year":"2003","journal-title":"Pediatr. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.neuroscience.2012.04.016","article-title":"Association of brain-derived neurotrophic factor and tyrosine kinase B receptor in pregnancy","volume":"216","author":"Dhobale","year":"2012","journal-title":"Neuroscience"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1542\/peds.2008-1222","article-title":"Differential patterns of 27 cord blood immune biomarkers across gestational age","volume":"123","author":"Matoba","year":"2009","journal-title":"Pediatrics"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ijdevneu.2014.06.005","article-title":"Neurotrophins: Role in adverse pregnancy outcome","volume":"37","author":"Dhobale","year":"2014","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ijdevneu.2017.10.001","article-title":"A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate","volume":"65","author":"Rajkumar","year":"2018","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"16372","DOI":"10.1523\/JNEUROSCI.2079-13.2013","article-title":"GDNF signaling levels control migration and neuronal differentiation of enteric ganglion precursors","volume":"33","author":"Uesaka","year":"2013","journal-title":"J. Neurosci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1006\/mvre.1999.2214","article-title":"Modulation of renal blood vessel formation by glial cell line-derived neurotrophic factor","volume":"59","author":"Kloth","year":"2000","journal-title":"Microvasc. Res."},{"key":"ref_69","first-page":"1389","article-title":"MiR-4505 aggravates lipopolysaccharide-induced vascular endothelial injury by targeting heat shock protein A12B","volume":"17","author":"Zhang","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"ref_70","first-page":"3393","article-title":"MicroRNA\u20114530 suppresses cell proliferation and induces apoptosis by targeting RASA1 in human umbilical vein endothelial cells","volume":"19","author":"Jing","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1080\/09168451.2015.1107460","article-title":"Identification of the differential expression of serum microRNA in type 2 diabetes","volume":"80","author":"Ding","year":"2016","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1089\/cmb.2019.0158","article-title":"Bioinformatics Analysis of Differentially Expressed Genes, Methylated Genes, and miRNAs in Unexplained Recurrent Spontaneous Abortion","volume":"26","author":"Chen","year":"2019","journal-title":"J. Comput. Biol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"111","DOI":"10.3892\/ol.2017.6102","article-title":"MicroRNA-4530 promotes angiogenesis by targeting VASH1 in breast carcinoma cells","volume":"14","author":"Zhang","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"437","DOI":"10.3324\/haematol.2015.134932","article-title":"Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasms","volume":"101","author":"Horiguchi","year":"2016","journal-title":"Haematologica"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Chen, L., Cao, P., Huang, C., Wu, Q., Chen, S., and Chen, F. (2020). Serum exosomal miR-7977 as a novel biomarker for lung adenocarcinoma. J. Cell Biochem., 1\u201310.","DOI":"10.1002\/jcb.29612"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1161\/CIRCRESAHA.108.191270","article-title":"MicroRNAs as novel regulators of angiogenesis","volume":"104","author":"Sessa","year":"2009","journal-title":"Circ. Res."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"7092151","DOI":"10.1155\/2019\/7092151","article-title":"Vascular Inflammation and Oxidative Stress: Major Triggers for Cardiovascular Disease","volume":"2019","author":"Steven","year":"2019","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1111\/apha.12416","article-title":"Application of microRNAs in diagnosis and treatment of cardiovascular disease","volume":"213","author":"Wronska","year":"2015","journal-title":"Acta Physiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1161\/CIRCRESAHA.114.305663","article-title":"Association of MicroRNAs and YRNAs With Platelet Function","volume":"118","author":"Kaudewitz","year":"2016","journal-title":"Circ. Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1373\/clinchem.2012.199711","article-title":"Evolving role of microparticles in the pathophysiology of endothelial dysfunction","volume":"59","author":"Lovren","year":"2013","journal-title":"Clin. Chem."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.imlet.2015.11.001","article-title":"MiR-150 impairs inflammatory cytokine production by targeting ARRB-2 after blocking CD28\/B7 costimulatory pathway","volume":"172","author":"Sang","year":"2016","journal-title":"Immunol. Lett."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Bai, R., Yang, Q., Xi, R., Li, L., Shi, D., and Chen, K. (2017). miR-941 as a promising biomarker for acute coronary syndrome. BMC Cardiovasc. Disord., 17.","DOI":"10.1186\/s12872-017-0653-8"},{"key":"ref_83","unstructured":"Foundation for the Accreditation of Cellular Therapy (FACT) (2020, January 16). Available online: www.factwebsite.org\/uploadedfiles\/news\/4th_edition_cord_blood_standards_4.0.pdf."},{"key":"ref_84","first-page":"563","article-title":"Clinical Evaluation of the Safety and Feasibility of Whole Autologous Cord Blood Transplant as a Source of Stem and Progenitor Cells for Extremely Premature Neonates: Preliminary Report","volume":"13","author":"Rudnicki","year":"2015","journal-title":"Exp. Clin. Transplant."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"\u0141uczkowska, K., Rogi\u0144ska, D., Ula\u0144czyk, Z., Paczkowska, E., Schmidt, C.A., and Machali\u0144ski, B. (2020). Molecular Mechanisms of Bortezomib Action: Novel Evidence for the miRNA-mRNA Interaction Involvement. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21010350"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"\u0141uczkowska, K., Rogi\u0144ska, D., Ula\u0144czyk, Z., and Machali\u0144ski, B. (2020). Effect of Bortezomib on Global Gene Expression in PC12-Derived Nerve Cells. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21030751"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/4\/1305\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:58:00Z","timestamp":1760173080000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/4\/1305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,14]]},"references-count":86,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["ijms21041305"],"URL":"https:\/\/doi.org\/10.3390\/ijms21041305","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,14]]}}}