{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T03:24:20Z","timestamp":1784949860214,"version":"3.55.0"},"reference-count":95,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T00:00:00Z","timestamp":1582848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The endogenous gasotransmitter H2S plays an important role in the central nervous, respiratory and cardiovascular systems. Accordingly, slow-releasing H2S donors are powerful tools for basic studies and innovative pharmaco-therapeutic agents for cardiovascular and neurodegenerative diseases. Nonetheless, the effects of H2S-releasing agents on the growth of stem cells have not been fully investigated. H2S preconditioning can enhance mesenchymal stem cell survival after post-ischaemic myocardial implantation; therefore, stem cell therapy combined with H2S may be relevant in cell-based therapy for regenerative medicine. Here, we studied the effects of slow-releasing H2S agents on the cell growth and differentiation of cardiac Lin\u2212 Sca1+ human mesenchymal stem cells (cMSC) and on normal human dermal fibroblasts (NHDF). In particular, we investigated the effects of water-soluble GSH\u2013garlic conjugates (GSGa) on cMSC compared to other H2S-releasing agents, such as Na2S and GYY4137. GSGa treatment of cMSC and NHDF increased their cell proliferation and migration in a concentration dependent manner with respect to the control. GSGa treatment promoted an upregulation of the expression of proteins involved in oxidative stress protection, cell\u2013cell adhesion and commitment to differentiation. These results highlight the effects of H2S-natural donors as biochemical factors that promote MSC homing, increasing their safety profile and efficacy after transplantation, and the value of these donors in developing functional 3D-stem cell delivery systems for cardiac muscle tissue repair and regeneration.<\/jats:p>","DOI":"10.3390\/ijms21051638","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T03:13:28Z","timestamp":1583205208000},"page":"1638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Glutathione\u2013Allylsulfur Conjugates as Mesenchymal Stem Cells Stimulating Agents for Potential Applications in Tissue Repair"],"prefix":"10.3390","volume":"21","author":[{"given":"Emilia","family":"Di Giovanni","sequence":"first","affiliation":[{"name":"Department of Chemical Science and Technologies, University of Rome Tor Vergata, via della Ricerca Scientifica 1, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Buonvino","sequence":"additional","affiliation":[{"name":"Department of Chemical Science and Technologies, University of Rome Tor Vergata, via della Ricerca Scientifica 1, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivano","family":"Amelio","sequence":"additional","affiliation":[{"name":"MRC Toxicology Unit, University of Cambridge, Cambridge CB2 1QP, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7694-5279","authenticated-orcid":false,"given":"Sonia","family":"Melino","sequence":"additional","affiliation":[{"name":"Department of Chemical Science and Technologies, University of Rome Tor Vergata, via della Ricerca Scientifica 1, 00133 Rome, Italy"},{"name":"CIMER Center for Regenerative Medicine, University of Rome Tor Vergata, via Montpellier 1, 00166 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1089\/ars.2006.8.661","article-title":"Hydrogen sulfide protects HT22 neuronal cells from oxidative stress","volume":"8","author":"Kimura","year":"2006","journal-title":"Antioxid. Redox. Signal."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1002\/med.20234","article-title":"Hydrogen sulphide: Novel opportunity for drug discovery","volume":"32","author":"Martelli","year":"2012","journal-title":"Med. Res. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.tibs.2015.08.007","article-title":"H2S: A Novel Gasotransmitter that Signals by Sulfhydration","volume":"40","author":"Paul","year":"2015","journal-title":"Trends Biochem. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1016\/S0006-291X(02)00422-9","article-title":"Brain hydrogen sulfide is severely decreased in Alzheimer\u2019s disease","volume":"293","author":"Eto","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1093\/jb\/mvp111","article-title":"Vascular endothelium expresses 3-mercaptopyruvate sulfurtransferase and produces hydrogen sulfide","volume":"146","author":"Shibuya","year":"2009","journal-title":"J. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1089\/ars.2011.4345","article-title":"Hydrogen sulfide is a signaling molecule and a cytoprotectant","volume":"17","author":"Kimura","year":"2012","journal-title":"Antioxid. Redox Signal."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1124\/mol.105.017467","article-title":"Direct stimulation of K(ATP) channels by exogenous and endogenous hydrogen sulfide in vascular smooth muscle cells","volume":"68","author":"Tang","year":"2005","journal-title":"Mol. Pharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.1161\/ATVBAHA.110.209783","article-title":"Hydrogen sulfide is an endogenous inhibitor of phosphodiesterase activity","volume":"30","author":"Bucci","year":"2010","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s12013-010-9079-y","article-title":"Homocysteine to hydrogen sulfide or hypertension","volume":"57","author":"Sen","year":"2010","journal-title":"Cell Biochem. Biophys."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.neuroscience.2007.12.008","article-title":"Hydrogen sulphide in the hypothalamus causes an ATP-sensitive K+ channel-dependent decrease in blood pressure in freely moving rats","volume":"152","author":"Dawe","year":"2008","journal-title":"Neuroscience"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nrd4433","article-title":"Hydrogen sulfide-based therapeutics: Exploiting a unique but ubiquitous gasotransmitter","volume":"14","author":"Wallace","year":"2010","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1007\/s10620-012-2051-5","article-title":"Gastroprotective effect of NaHS against mucosal lesions induced by ischemia-reperfusion injury in rat","volume":"57","author":"Mard","year":"2012","journal-title":"Dig. Dis. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"17977","DOI":"10.1073\/pnas.0705710104","article-title":"Hydrogen sulfide mediates the vasoactivity of garlic","volume":"104","author":"Benavides","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1096\/fj.04-2279fje","article-title":"Hydrogen sulfide-induced apoptosis of human aorta smooth muscle cells via the activation of mitogen-activated protein kinases and caspase-3","volume":"18","author":"Yang","year":"2004","journal-title":"Faseb. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1089\/ars.2009.2915","article-title":"Butyrate-stimulated H2S production in colon cancer cells","volume":"12","author":"Cao","year":"2010","journal-title":"Antioxid Redox Signal."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.3390\/molecules20011731","article-title":"Glutathione-garlic sulfur conjugates: Slow hydrogen sulfide releasing agents for therapeutic applications","volume":"20","author":"Bhuiyan","year":"2015","journal-title":"Molecules"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.freeradbiomed.2018.12.025","article-title":"Effects of sulfane sulfur content in benzyl polysulfides on thiol-triggered H2S release and cell proliferation","volume":"131","author":"Bolton","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3990","DOI":"10.3748\/wjg.v11.i26.3990","article-title":"Hydrogen sulfide protects colon cancer cells from chemopreventative agent beta-phenylethyl isothiocyanate induced apoptosis","volume":"11","author":"Rose","year":"2005","journal-title":"World J. Gastroenterol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1042\/CBI20090368","article-title":"Hydrogen sulfide induces human colon cancer cell proliferation: Role of Akt, ERK and p21","volume":"34","author":"Cai","year":"2007","journal-title":"Cell. Biol. Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"H2693","DOI":"10.1152\/ajpheart.00853.2007","article-title":"S-allylcysteine mediates cardioprotection in an acute myocardial infarction rat model via a hydrogen sulfide-mediated pathway","volume":"293","author":"Chuah","year":"2007","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1096\/fj.02-0883fje","article-title":"Hydrogen sulfide induces serum-independent cell cycle entry in nontransformed rat intestinal epithelial cells","volume":"17","author":"Deplancke","year":"2003","journal-title":"Faseb. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1124\/mol.108.047985","article-title":"Hydrogen sulfide inhibits rotenone-induced apoptosis via preservation of mitochondrial function","volume":"75","author":"Hu","year":"2009","journal-title":"Mol. Pharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/j.1749-6632.2012.06743.x","article-title":"Molecular mechanisms of garlic-derived allyl sulfides in the inhibition of skin cancer progression","volume":"1271","author":"Wang","year":"2012","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1207\/S15327914NC422_14","article-title":"Z-ajoene induces apoptosis of HL-60 cells: Involvement of Bcl-2 cleavage","volume":"42","author":"Li","year":"2002","journal-title":"Nutr. Cancer"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.3892\/or.2014.3361","article-title":"Diallyl disulfide induces G2\/M arrest and promotes apoptosis through the p53\/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma","volume":"32","author":"Yin","year":"2014","journal-title":"Oncol. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"6256","DOI":"10.1038\/sj.onc.1208759","article-title":"Diallyl trisulfide-induced G(2)-M phase cell cycle arrest in human prostate cancer cells is caused by reactive oxygen species-dependent destruction and hyperphosphorylation of Cdc 25 C","volume":"24","author":"Xiao","year":"2005","journal-title":"Oncogene"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1002\/em.20431","article-title":"Diallyl trisulfide selectively causes Bax- and Bak-mediated apoptosis in human lung cancer cells","volume":"50","author":"Xiao","year":"2009","journal-title":"Environ. Mol. Mutagen."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.3892\/ijo.2012.1656","article-title":"Diallyl trisulfide sensitizes human melanoma cells to TRAIL-induced cell death by promoting endoplasmic reticulum-mediated apoptosis","volume":"41","author":"Murai","year":"2012","journal-title":"Int. J. Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s10549-013-2440-2","article-title":"Critical role for reactive oxygen species in apoptosis induction and cell migration inhibition by diallyl trisulfide, a cancer chemopreventive component of garlic","volume":"138","author":"Lee","year":"2013","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1124\/mol.53.3.402","article-title":"Ajoene, a compound of garlic, induces apoptosis in human promyeloleukemic cells, accompanied by generation of reactive oxygen species and activation of nuclear factor kappaB","volume":"53","author":"Dirsch","year":"1998","journal-title":"Mol. Pharmacol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1510\/icvts.2009.219535","article-title":"Effect of hydrogen sulfide on myocardial protection in the setting of cardioplegia and cardiopulmonary bypass","volume":"10","author":"Osipov","year":"2010","journal-title":"Interact. Cardiovasc. Thorac. Surg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"15560","DOI":"10.1073\/pnas.0705891104","article-title":"Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function","volume":"104","author":"Elrod","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.imlet.2011.10.004","article-title":"High concentrations of hydrogen sulphide elevate the expression of a series of pro-inflammatory genes in fibroblast-like synoviocytes derived from rheumatoid and osteoarthritis patients","volume":"141","author":"Kloesch","year":"2012","journal-title":"Immunol. Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.freeradbiomed.2006.03.021","article-title":"Hydrogen sulfide inhibits nitric oxide production and nuclear factor-kappaB via heme oxygenase-1 expression in RAW264.7 macrophages stimulated with lipopolysaccharide","volume":"41","author":"Oh","year":"2006","journal-title":"Free Radic. Biol. Med."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Pan, L.L., Liu, X.H., Gong, Q.H., Wu, D., and Zhu, Y.Z. (2011). Hydrogen sulfide attenuated tumor necrosis factor-alpha-induced inflammatory signaling and dysfunction in vascular endothelial cells. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0019766"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1111\/j.1440-1681.2010.05351.x","article-title":"Interaction of hydrogen sulfide with ion channels","volume":"37","author":"Tang","year":"2010","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1089\/ars.2012.4612","article-title":"Hydrogen sulfide in biochemistry and medicine","volume":"17","author":"Predmore","year":"2012","journal-title":"Antioxid. Redox Signal."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Cacciotti, I., Ciocci, M., Di Giovanni, E., Nanni, F., and Melino, S. (2018). Hydrogen Sulfide-Releasing Fibrous Membranes: Potential Patches for Stimulating Human Stem Cells Proliferation and Viability under Oxidative Stress. Int. J. Mol. Sci., 19.","DOI":"10.20944\/preprints201807.0079.v1"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1002\/mabi.201500430","article-title":"Design of a Novel Composite H2 S-Releasing Hydrogel for Cardiac Tissue Repair","volume":"16","author":"Mauretti","year":"2016","journal-title":"Macromol. Biosci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"84338","DOI":"10.18632\/oncotarget.12609","article-title":"H2S-releasing nanoemulsions: A new formulation to inhibit tumor cells proliferation and improve tissue repair","volume":"7","author":"Ciocci","year":"2016","journal-title":"Oncotarget"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1096\/fj.04-1815fje","article-title":"Hydrogen sulfide protects neurons from oxidative stress","volume":"18","author":"Kimura","year":"2004","journal-title":"Faseb. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.neuint.2013.09.003","article-title":"Physiological role of hydrogen sulfide and polysulfide in the central nervous system","volume":"63","author":"Kimura","year":"2013","journal-title":"Neurochem. Int."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1111\/j.1471-4159.2006.04283.x","article-title":"Hydrogen sulfide attenuates lipopolysaccharide-induced inflammation by inhibition of p38 mitogen-activated protein kinase in microglia","volume":"100","author":"Hu","year":"2007","journal-title":"J. Neurochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.3892\/mmr.2014.1923","article-title":"Cx43 in mesenchymal stem cells promotes angiogenesis of the infarcted heart independent of gap junctions","volume":"9","author":"Wang","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"58089","DOI":"10.18632\/oncotarget.11166","article-title":"Preconditioning of bone marrow mesenchymal stem cells with hydrogen sulfide improves their therapeutic potential","volume":"7","author":"Zhang","year":"2016","journal-title":"Oncotarget"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1111\/j.1471-4159.2004.02617.x","article-title":"The novel neuromodulator hydrogen sulfide: An endogenous peroxynitrite \u2018scavenger\u2019?","volume":"90","author":"Whiteman","year":"2004","journal-title":"J. Neurochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3548","DOI":"10.1016\/j.febslet.2013.09.013","article-title":"Polysulfide exerts a protective effect against cytotoxicity caused by t-buthylhydroperoxide through Nrf2 signaling in neuroblastoma cells","volume":"587","author":"Koike","year":"2013","journal-title":"FEBS Lett."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1160\/TH10-10-0630","article-title":"Expression of the vitamin K-dependent proteins GAS6 and protein S and the TAM receptor tyrosine kinases in human atherosclerotic carotid plaques","volume":"105","author":"Hurtado","year":"2011","journal-title":"Thromb. Haemost."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/j.jacc.2009.11.016","article-title":"Mutations in alpha-actinin-2 cause hypertrophic cardiomyopathy: A genome-wide analysis","volume":"55","author":"Chiu","year":"2010","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1172\/JCI9224","article-title":"Mutations in the human delta-sarcoglycan gene in familial and sporadic dilated cardiomyopathy","volume":"106","author":"Tsubata","year":"2000","journal-title":"J. Clin. Invest."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.ejheart.2008.04.014","article-title":"Mosaic inactivation of the serum response factor gene in the myocardium induces focal lesions and heart failure","volume":"10","author":"Parlakian","year":"2008","journal-title":"Eur. J. Heart Fail."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.pbb.2013.11.009","article-title":"Hydrogen sulfide promotes proliferation and neuronal differentiation of neural stem cells and protects hypoxia-induced decrease in hippocampal neurogenesis","volume":"116","author":"Liu","year":"2014","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1089\/ars.2009.2899","article-title":"The effect of hydrogen sulfide donors on lipopolysaccharide-induced formation of inflammatory mediators in macrophages","volume":"12","author":"Whiteman","year":"2010","journal-title":"Antioxid Redox Signal."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"11601","DOI":"10.1074\/jbc.M808026200","article-title":"H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia","volume":"284","author":"Chiku","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"22457","DOI":"10.1074\/jbc.M109.010868","article-title":"Relative contributions of cystathionine beta-synthase and gamma-cystathionase to H2S biogenesis via alternative trans-sulfuration reactions","volume":"284","author":"Singh","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3076","DOI":"10.1074\/jbc.M805459200","article-title":"Structural basis for the inhibition mechanism of human cystathionine gamma-lyase, an enzyme responsible for the production of H(2)S","volume":"284","author":"Sun","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"5510","DOI":"10.1073\/pnas.92.12.5510","article-title":"Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension","volume":"92","author":"Wang","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_58","first-page":"176","article-title":"Hydroxylation of HIF-1: Oxygen sensing at the molecular level","volume":"19","author":"Semenza","year":"2004","journal-title":"Physiology (Bethesda)"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/10409238.2013.838205","article-title":"Transcriptional regulation by hypoxia inducible factors","volume":"49","author":"Dengler","year":"2013","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Yang, C., Yang, Z., Zhang, M., Dong, Q., Wang, X., Lan, A., Zeng, F., Chen, P., Wang, C., and Feng, J. (2011). Hydrogen sulfide protects against chemical hypoxia-induced cytotoxicity and inflammation in HaCaT cells through inhibition of ROS\/NF-kappaB\/COX-2 pathway. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0021971"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1097\/01.shk.0000183393.83272.de","article-title":"Oxidative stress-dependent conversion of hydrogen sulfide to sulfite by activated neutrophils","volume":"24","author":"Mitsuhashi","year":"2005","journal-title":"Shock"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1016\/j.bbrc.2004.04.094","article-title":"Endogenous hydrogen sulfide regulation of myocardial injury induced by isoproterenol","volume":"318","author":"Geng","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3352","DOI":"10.1111\/j.1742-4658.2008.06482.x","article-title":"Three enzymatic activities catalyze the oxidation of sulfide to thiosulfate in mammalian and invertebrate mitochondria","volume":"275","author":"Hildebrandt","year":"2008","journal-title":"Febs. J."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1038\/nm.1907","article-title":"Loss of ETHE1, a mitochondrial dioxygenase, causes fatal sulfide toxicity in ethylmalonic encephalopathy","volume":"15","author":"Tiranti","year":"2009","journal-title":"Nat. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/nm.2188","article-title":"Combined treatment with oral metronidazole and N-acetylcysteine is effective in ethylmalonic encephalopathy","volume":"16","author":"Viscomi","year":"2010","journal-title":"Nat. Med."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Siegel, D., Dehn, D.D., Bokatzian, S.S., Quinn, K., Backos, D.S., Di Francesco, A., Bernier, M., Reisdorph, N., de Cabo, R., and Ross, D. (2018). Redox modulation of NQO1. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0190717"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.1073\/pnas.052706799","article-title":"NQO1 stabilizes p53 through a distinct pathway","volume":"99","author":"Asher","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1089\/ars.2010.3211","article-title":"Regulation of the Nrf2-Keap1 antioxidant response by the ubiquitin proteasome system: An insight into cullin-ring ubiquitin ligases","volume":"13","author":"Villeneuve","year":"2010","journal-title":"Antioxid. Redox Signal."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"30091","DOI":"10.1074\/jbc.M501279200","article-title":"Ubiquitination of Keap1, a BTB-Kelch substrate adaptor protein for Cul3, targets Keap1 for degradation by a proteasome-independent pathway","volume":"280","author":"Zhang","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"3171","DOI":"10.2337\/db16-0020","article-title":"Hydrogen Sulfide Induces Keap1 S-sulfhydration and Suppresses Diabetes-Accelerated Atherosclerosis via Nrf2 Activation","volume":"65","author":"Xie","year":"2016","journal-title":"Diabetes"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.phrs.2012.04.005","article-title":"ERK1\/2 MAP kinases: Structure, function, and regulation","volume":"66","author":"Roskoski","year":"2012","journal-title":"Pharmacol Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1478-811X-9-23","article-title":"The secret life of kinases: Functions beyond catalysis","volume":"9","author":"Rauch","year":"2011","journal-title":"Cell Commun. Signal."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"21972","DOI":"10.1073\/pnas.0908047106","article-title":"Hydrogen sulfide is an endogenous stimulator of angiogenesis","volume":"106","author":"Papapetropoulos","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1111\/jcmm.12077","article-title":"Crosstalk between hydrogen sulfide and nitric oxide in endothelial cells","volume":"17","author":"Altaany","year":"2013","journal-title":"J. Cell Mol. Med."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1039\/C5MB00101C","article-title":"The PI3K\/AKT\/mTOR interactive pathway","volume":"11","author":"Ersahin","year":"2015","journal-title":"Mol. Biosyst."},{"key":"ref_76","first-page":"3715037","article-title":"Hydrogen Sulfide Prevents Formation of Reactive Oxygen Species through PI3K\/Akt Signaling and Limits Ventilator-Induced Lung Injury","volume":"2017","author":"Spassov","year":"2017","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.cardiores.2006.07.016","article-title":"Progressive increase in conduction velocity across human mesenchymal stem cells is mediated by enhanced electrical coupling","volume":"72","author":"Pijnappels","year":"2006","journal-title":"Cardiovasc. Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1161\/CIRCULATIONAHA.108.827691","article-title":"Sca-1+ stem cell survival and engraftment in the infarcted heart: Dual role for preconditioning-induced connexin-43","volume":"119","author":"Lu","year":"2009","journal-title":"Circulation"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.neuint.2013.11.002","article-title":"Role of hydrogen sulfide in secondary neuronal injury","volume":"64","author":"Wang","year":"2014","journal-title":"Neurochem. Int."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.jacc.2007.11.040","article-title":"Pre-treatment of mesenchymal stem cells with a combination of growth factors enhances gap junction formation, cytoprotective effect on cardiomyocytes, and therapeutic efficacy for myocardial infarction","volume":"51","author":"Hahn","year":"2008","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1159\/000480411","article-title":"Hydrogen Sulfide-Preconditioning of Human Endothelial Progenitor Cells Transplantation Improves Re-Endothelialization in Nude Mice with Carotid Artery Injury","volume":"43","author":"Ke","year":"2017","journal-title":"Cell Physiol. Biochem."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1620\/tjem.226.29","article-title":"Transplantation of mesenchymal stem cells preconditioned with hydrogen sulfide enhances repair of myocardial infarction in rats","volume":"226","author":"Xie","year":"2012","journal-title":"Tohoku J. Exp. Med."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1161\/CIRCULATIONAHA.116.022967","article-title":"Restoration of Hydrogen Sulfide Production in Diabetic Mice Improves Reparative Function of Bone Marrow Cells","volume":"134","author":"Cheng","year":"2016","journal-title":"Circulation"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1523\/JNEUROSCI.16-03-01066.1996","article-title":"The possible role of hydrogen sulfide as an endogenous neuromodulator","volume":"16","author":"Abe","year":"1996","journal-title":"J. Neurosci"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1021\/cn500185g","article-title":"Hydrogen sulfide: A neuromodulator and neuroprotectant in the central nervous system","volume":"5","author":"Zhang","year":"2014","journal-title":"ACS Chem. Neurosci."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nprot.2008.229","article-title":"Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: An in vitro model for studying human cardiac physiology and pathophysiology","volume":"4","author":"Smits","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"686","DOI":"10.3389\/fphar.2017.00686","article-title":"The Role of Hydrogen Sulfide on Cardiovascular Homeostasis: An Overview with Update on Immunomodulation","volume":"8","author":"Pan","year":"2017","journal-title":"Front. Pharmacol"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0022-1759(86)90368-6","article-title":"Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability","volume":"89","author":"Denizot","year":"1986","journal-title":"J. Immunol. Methods"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1007\/s10616-015-9909-2","article-title":"A comparative study of colorimetric cell proliferation assays in immune cells","volume":"68","author":"Koyanagi","year":"2016","journal-title":"Cytotechnology"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"2051","DOI":"10.1002\/stem.763","article-title":"Human cardiac progenitor cell grafts as unrestricted source of supernumerary cardiac cells in healthy murine hearts","volume":"29","author":"Forte","year":"2011","journal-title":"Stem Cells"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID Bioinformatics Resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nature Protoc."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/nar\/gkn923","article-title":"Bioinformatics enrichment tools: Paths toward the comprehensive functional analysis of large gene lists","volume":"37","author":"Huang","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s13287-018-1130-8","article-title":"Mesenchymal stem cell 3D encapsulation technologies for biomimetic microenvironment in tissue regeneration","volume":"10","author":"Kim","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nrd.2016.193","article-title":"Tumour-associated mesenchymal stem\/stromal cells: Emerging therapeutic targets","volume":"16","author":"Shi","year":"2017","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41581-018-0023-5","article-title":"Immunoregulatory mechanisms of mesenchymal stem and stromal cells in inflammatory diseases","volume":"14","author":"Shi","year":"2018","journal-title":"Nat. Rev. Nephrol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/5\/1638\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:02:38Z","timestamp":1760173358000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/5\/1638"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,28]]},"references-count":95,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,3]]}},"alternative-id":["ijms21051638"],"URL":"https:\/\/doi.org\/10.3390\/ijms21051638","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,28]]}}}