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Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.<\/jats:p>","DOI":"10.3390\/ijms21062027","type":"journal-article","created":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T08:13:27Z","timestamp":1584519207000},"page":"2027","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Recent Advances in Practical Methods for Liver Cell Biology: A Short Overview"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2894-3188","authenticated-orcid":false,"given":"Sandra","family":"Torres","sequence":"first","affiliation":[{"name":"Department of Internal Medicine I, Goethe University Frankfurt, 60590 Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinab","family":"Abdullah","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine and Experimental Immunology, University Clinic, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian J","family":"Brol","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, University Clinic, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Hellerbrand","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Friedrich-Alexander University Erlangen-N\u00fcrnberg, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Angiogenesis in Liver Disease Research Group, IDIBAPS Biomedical Research Institute, University of Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romina","family":"Fiorotto","sequence":"additional","affiliation":[{"name":"Yale University School of Medicine, Yale Liver Center, Department of Internal Medicine, New Haven, CT 06520, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Goethe University Frankfurt, 60590 Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"K\u00f6nigshofer","sequence":"additional","affiliation":[{"name":"Div. of Gastroenterology and Hepatology, Dept. of Internal Medicine III, Medical University of Vienna, A-1090 Vienna, Austria"},{"name":"HEPEX Lab for Liver Fibrosis and Portal Hypertension, Medical University of Vienna, A-1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Liedtke","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital RWTH Aachen, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Lotersztajn","sequence":"additional","affiliation":[{"name":"Inserm UMR-1149, Centre de Recherche sur l\u2019Inflammation, 75890 Paris, France"},{"name":"Sorbonne Paris Cit\u00e9, Laboratoire d\u2019Excellence Inflamex, Facult\u00e9 de M\u00e9decine, Site Xavier Bichat, Universit\u00e9 Paris Diderot, 75018 Paris, France"},{"name":"Hospitalo-Universitaire UNITY, Service d\u2019H\u00e9patologie, H\u00f4pital Beaujon, Assistance Publique-H\u00f4pitaux de Paris, 92110 Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia A.","family":"Nevzorova","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital RWTH Aachen, 52074 Aachen, Germany"},{"name":"Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Schierwagen","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Goethe University Frankfurt, 60590 Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Reiberger","sequence":"additional","affiliation":[{"name":"Div. of Gastroenterology and Hepatology, Dept. of Internal Medicine III, Medical University of Vienna, A-1090 Vienna, Austria"},{"name":"HEPEX Lab for Liver Fibrosis and Portal Hypertension, Medical University of Vienna, A-1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank Erhard","family":"Uschner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Goethe University Frankfurt, 60590 Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Tacke","sequence":"additional","affiliation":[{"name":"Department of Hepatology & Gastroenterology, Charit\u00e9 University Medicine Berlin, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3888-0931","authenticated-orcid":false,"given":"Ralf","family":"Weiskirchen","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, (IFMPEGKC), University Hospital Aachen, D-52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonel","family":"Trebicka","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Goethe University Frankfurt, 60590 Frankfurt, Germany"},{"name":"European Foundation for Study of Chronic Liver Failure, 08021 Barcelona, Spain"},{"name":"Institute of Bioengineering Catalunya, 08028 Barcelona, Spain"},{"name":"University of Southern Denmark, 5230 Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Endo-Umeda, K., and Makishima, M. 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