{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:52:20Z","timestamp":1791219140793,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,3,23]],"date-time":"2020-03-23T00:00:00Z","timestamp":1584921600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006364","name":"Institut National Du Cancer","doi-asserted-by":"publisher","award":["6001"],"award-info":[{"award-number":["6001"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ligue Nationale contre le Cancer","award":["Financement 2018-19 CGO comit\u00e9s 22, 44 et 49"],"award-info":[{"award-number":["Financement 2018-19 CGO comit\u00e9s 22, 44 et 49"]}]},{"DOI":"10.13039\/100011719","name":"Bone Cancer Research Trust","doi-asserted-by":"publisher","award":["144681"],"award-info":[{"award-number":["144681"]}],"id":[{"id":"10.13039\/100011719","id-type":"DOI","asserted-by":"publisher"}]},{"name":"SFODF (Soci\u00e9t\u00e9 Fran\u00e7aise d'Orthodontie Dento-Faciale","award":["Prix Recherche 2017-18"],"award-info":[{"award-number":["Prix Recherche 2017-18"]}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["Recurent"],"award-info":[{"award-number":["Recurent"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University Paris-Diderot","award":["Recurent"],"award-info":[{"award-number":["Recurent"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The purpose of the present study was to assess the early stages of development of mouse first molar roots in the osteopetrotic context of RANKL invalidation in order to demonstrate that the radicular phenotype observed resulted not only from defective osteoclasts, but also from loss of cell-to-cell communication among dental, periodontium and alveolar bone cells involving RANKL signaling. Two experimental models were used in this study: Rankl mutants with permanent RANKL invalidation, and C57BL\/6J mice injected during the first postnatal week with a RANKL neutralizing antibody corresponding to a transient RANKL invalidation. The dento-alveolar complex was systematically analyzed using micro-CT, and histological and immunohistochemical approaches. These experiments showed that the root elongation alterations observed in the Rankl-\/- mice were associated with reduced proliferation of the RANK-expressing HERS cells with a significant decrease in proliferating cell nuclear antigen (PCNA) expression and a significant increase in P21 expression. The phenotypic comparison of the adult first molar root at 35 days between permanent and transitory invalidations of RANKL made it possible to demonstrate that alterations in dental root development have at least two origins, one intrinsic and linked to proliferation\/differentiation perturbations in dental-root-forming cells, the other extrinsic and corresponding to disturbances of bone cell differentiation\/function.<\/jats:p>","DOI":"10.3390\/ijms21062201","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T07:16:08Z","timestamp":1585034168000},"page":"2201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Origins of Alterations to Rankl Null Mutant Mouse Dental Root Development"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7403-9720","authenticated-orcid":false,"given":"Andrea","family":"Gama","sequence":"first","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM UMR-1138, Sorbonne Universit\u00e9, Universit\u00e9 de Paris, Laboratoire de Physiopathologie Orale Mol\u00e9culaire, F-75006 Paris, France"},{"name":"Odontologic Center of District Federal Military Police, Bras\u00edlia 70297-400, Brazil"},{"name":"Laboratory of Oral Histopathology, Health Sciences Faculty, University of Bras\u00edlia, Bras\u00edlia 70910-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge William","family":"Vargas-Franco","sequence":"additional","affiliation":[{"name":"INSERM, UMR-1238, Equipe 1, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Nantes, F-44035 Nantes, France"},{"name":"Department of Basic Studies, Faculty of Odontology, University of Antioquia, Medellin A.A1226, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Carolina","family":"S\u00e1nchez Mesa","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM UMR-1138, Sorbonne Universit\u00e9, Universit\u00e9 de Paris, Laboratoire de Physiopathologie Orale Mol\u00e9culaire, F-75006 Paris, France"},{"name":"Department of Orthodontics, Faculty of Odontology, University of Antioquia, Medellin A.A1226, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Restrepo Bedoya","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM UMR-1138, Sorbonne Universit\u00e9, Universit\u00e9 de Paris, Laboratoire de Physiopathologie Orale Mol\u00e9culaire, F-75006 Paris, France"},{"name":"Department of Orthodontics, Faculty of Odontology, University of Antioquia, Medellin A.A1226, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9rome","family":"Amiaud","sequence":"additional","affiliation":[{"name":"INSERM, UMR-1238, Equipe 1, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Nantes, F-44035 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Babajko","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM UMR-1138, Sorbonne Universit\u00e9, Universit\u00e9 de Paris, Laboratoire de Physiopathologie Orale Mol\u00e9culaire, F-75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Berdal","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM UMR-1138, Sorbonne Universit\u00e9, Universit\u00e9 de Paris, Laboratoire de Physiopathologie Orale Mol\u00e9culaire, F-75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7110-7110","authenticated-orcid":false,"given":"Ana Carolina","family":"Acevedo","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Histopathology, Health Sciences Faculty, University of Bras\u00edlia, Bras\u00edlia 70910-900, Brazil"},{"name":"Oral Center for Inherited Diseases, Hospital of University of Brasilia, University of Bras\u00edlia, Bras\u00edlia 70910-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7777-0669","authenticated-orcid":false,"given":"Dominique","family":"Heymann","sequence":"additional","affiliation":[{"name":"INSERM, LEA Sarcoma Research Unit, University of Sheffield, Department of Oncology and Human Metabolism, Medical School, Sheffield S10 2RX, UK"},{"name":"INSERM, UMR-1232, LabCT, CRCNA, Universit\u00e9 de Nantes, Universit\u00e9 d\u2019Angers, Institut de Canc\u00e9rologie de l\u2019Ouest, site Ren\u00e9 Gauducheau, F-44805 Saint-Herblain, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6274-6981","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"L\u00e9zot","sequence":"additional","affiliation":[{"name":"INSERM, UMR-1238, Equipe 1, Facult\u00e9 de M\u00e9decine, Universit\u00e9 de Nantes, F-44035 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Castaneda","sequence":"additional","affiliation":[{"name":"Service d\u2019Odontologie-Stomatologie, H\u00f4pital Piti\u00e9-Salp\u00eatri\u00e8re, AP-HP, F-75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1177\/0022034513510321","article-title":"Interactions of the tooth and bone during 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