{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:37:41Z","timestamp":1790408261710,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T00:00:00Z","timestamp":1585180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Dental pulp stem cells (DPSCs) have excellent proliferative properties, mineralization potential and can be easily obtained from third molar teeth. Recently, many studies have focused on isolation and differentiation of DPSCs. In our study, we focused on biological properties of non-differentiated DPSCs in comparison with osteogenic differentiated cells from DPSCs. We analyzed morphology as well as mineralization potential using three varied osteogenic differentiation media. After fifteen days of differentiation, calcium deposit production was observed in all three osteogenic differentiation media. However, only one osteogenic medium, without animal serum supplement, showed rapid and strong calcification\u2014OsteoMAX-XF\u2122 Differentiation Medium. Therefore, we examined specific surface markers, and gene and protein expression of cells differentiated in this osteogenic medium, and compared them to non-differentiated DPSCs. We proved a decrease in expression of CD9 and CD90 mesenchymal stem cell surface markers, as well as downregulation in the expression of pluripotency genes (NANOG and OCT-4) and increased levels of expression in osteogenic genes (ALP, BSP, OCN and RUNX2). Moreover, osteogenic proteins, such as BSP and OCN, were only produced in differentiated cells. Our findings confirm that carefully selected differentiation conditions for stem cells are essential for their translation into future clinical applications.<\/jats:p>","DOI":"10.3390\/ijms21072280","type":"journal-article","created":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T11:36:42Z","timestamp":1585654602000},"page":"2280","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["A Comparative In Vitro Analysis of the Osteogenic Potential of Human Dental Pulp Stem Cells Using Various Differentiation Conditions"],"prefix":"10.3390","volume":"21","author":[{"given":"Terezia","family":"Okajcekova","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"},{"name":"Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Strnadel","sequence":"additional","affiliation":[{"name":"Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Pokusa","sequence":"additional","affiliation":[{"name":"Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romana","family":"Zahumenska","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"},{"name":"Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Janickova","sequence":"additional","affiliation":[{"name":"Department of Stomatology and Maxillofacial Surgery, University Hospital in Martin, 03601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Halasova","sequence":"additional","affiliation":[{"name":"Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"},{"name":"Department of Medical Biology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrieta","family":"Skovierova","sequence":"additional","affiliation":[{"name":"Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dimitriou, R., Jones, E., McGonagle, D., and Giannoudis, P.V. (2011). Bone regeneration: Current concepts and future directions. BMC Med., 9.","DOI":"10.1186\/1741-7015-9-66"},{"key":"ref_2","first-page":"12","article-title":"The Osteogenic Potential of Human Nondifferentiated and Pre-differentiated Mesenchymal Stem Cells Combined with an Osteoconductive Scaffold\u2014Early Stage Healing","volume":"60","year":"2017","journal-title":"Acta Medica"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.jcyt.2017.12.011","article-title":"Progress in the use of dental pulp stem cells in regenerative medicine","volume":"20","author":"Anitua","year":"2018","journal-title":"Cytotherapy"},{"key":"ref_4","first-page":"4372","article-title":"Isolation, characterization and comparative differentiation of human dental pulp stem cells derived from permanent teeth by using two different methods","volume":"24","author":"Karamzadeh","year":"2012","journal-title":"J. Vis. Exp."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13625","DOI":"10.1073\/pnas.240309797","article-title":"Postnatal human dental pulp stem cells (DPSCs) In Vitro and In Vivo","volume":"97","author":"Gronthos","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Iwamoto, T., Nakamura, T., Ishikawa, M., Yoshizaki, K., Sugimoto, A., Ida-Yonemochi, H., Ohshima, H., Saito, M., Yamada, Y., and Fukumoto, S. (2017). Pannexin 3 regulates proliferation and differentiation of odontoblasts via its hemichannel activities. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0177557"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Chieruzzi, M., Pagano, S., Moretti, S., Pinna, R., Milia, E., Torre, L., and Eramo, S. (2016). Nanomaterials for Tissue Engineering in Dentistry. Nanomaterials, 6.","DOI":"10.3390\/nano6070134"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1051\/sicotj\/2016004","article-title":"Dental pulp-derived stromal cells exhibit a higher osteogenic potency than bone marrow-derived stromal cells In Vitro and in a porcine critical-size bone defect model","volume":"2","author":"Jensen","year":"2016","journal-title":"SICOT J."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Yamada, Y., Nakamura-Yamada, S., Kusano, K., and Baba, S. (2019). Clinical Potential and Current Progress of Dental Pulp Stem Cells for Various Systemic Diseases in Regenerative Medicine: A Concise Review. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20051132"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5430","DOI":"10.1038\/s41598-019-41741-w","article-title":"Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation","volume":"9","author":"Noda","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_11","first-page":"195","article-title":"Human dental pulp stem cells isolation and long-term cultivation","volume":"50","author":"Soukup","year":"2007","journal-title":"Acta Medica"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s41232-017-0039-4","article-title":"Isolation of dental pulp stem cells with high osteogenic potential","volume":"37","author":"Yasui","year":"2017","journal-title":"Inflamm. Regen."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ajlan, S.A., Ashri, N.Y., Aldahmash, A.M., and Alnbaheen, M.S. (2015). Osteogenic differentiation of dental pulp stem cells under the influence of three different materials. BMC Oral Health., 15.","DOI":"10.1186\/s12903-015-0113-8"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.bbrc.2018.04.213","article-title":"Comparative characterization of stem cells from human exfoliated deciduous teeth, dental pulp, and bone marrow-derived mesenchymal stem cells","volume":"501","author":"Kunimatsu","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1166\/jnn.2014.9127","article-title":"Scaffold design for bone regeneration","volume":"14","year":"2014","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_16","first-page":"302","article-title":"Therapeutic potential of dental pulp stem cells in regenerative medicine: An overview","volume":"11","author":"Verma","year":"2014","journal-title":"Dent. Res. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"839","DOI":"10.4252\/wjsc.v7.i5.839","article-title":"Human dental pulp stem cells: Applications in future regenerative medicine","volume":"7","author":"Potdar","year":"2015","journal-title":"World J. Stem Cells"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"16570","DOI":"10.1038\/srep16570","article-title":"Optimization of human mesenchymal stem cell manufacturing: The effects of animal\/xeno-free media","volume":"5","author":"Oikonomopoulos","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6597815","DOI":"10.1155\/2017\/6597815","article-title":"Xeno-Free Strategies for Safe Human Mesenchymal Stem\/Stromal Cell Expansion: Supplements and Coatings","volume":"2017","author":"Cimino","year":"2017","journal-title":"Stem Cells Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1186\/s13287-015-0162-6","article-title":"Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum","volume":"6","author":"Sillat","year":"2015","journal-title":"Stem Cell Res. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jpor.2012.06.001","article-title":"Stem cells in dentistry\u2014Part I: Stem cell sources","volume":"56","author":"Egusa","year":"2012","journal-title":"J. Prosthodont. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13287-016-0359-3","article-title":"A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells","volume":"7","author":"Moraes","year":"2016","journal-title":"Stem Cell Res. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"118","DOI":"10.15283\/ijsc.2014.7.2.118","article-title":"Comparison of Mesenchymal Stem Cell Markers in Multiple Human Adult Stem Cells","volume":"7","author":"Maleki","year":"2014","journal-title":"Int. J. Stem Cells"},{"key":"ref_24","first-page":"1109","article-title":"Commonly used mesenchymal stem cell markers and tracking labels: Limitations and challenges","volume":"28","author":"Lin","year":"2013","journal-title":"Histol. Histopathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/s12291-017-0641-x","article-title":"Characterization and Classification of Mesenchymal Stem Cells in Several Species Using Surface Markers for Cell Therapy Purposes","volume":"33","author":"Ghaneialvar","year":"2018","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/oxfordjournals.jbchem.a002824","article-title":"Osteoblast-related gene expression of bone marrow cells during the osteoblastic differentiation induced by type I collagen","volume":"129","author":"Mizuno","year":"2001","journal-title":"J. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1615\/CritRevEukarGeneExpr.v19.i4.30","article-title":"Gene expression in stem cells","volume":"19","author":"Liang","year":"2009","journal-title":"Crit. Rev. Eukaryot. Gene Expr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/S0168-9525(03)00176-8","article-title":"Transcriptional mechanisms in osteoblast differentiation and bone formation","volume":"19","author":"Nakashima","year":"2003","journal-title":"Trends Genet."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1034\/j.1601-0825.2002.01829.x","article-title":"Regulatory mechanisms of osteoblast and osteoclast differentiation","volume":"8","author":"Katagiri","year":"2002","journal-title":"Oral Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"95","DOI":"10.12659\/MSMBR.901142","article-title":"Osteoblast Differentiation at a Glance","volume":"22","author":"Rutkovskiy","year":"2016","journal-title":"Med. Sci. Monit. 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