{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:48:28Z","timestamp":1760237308473,"version":"build-2065373602"},"reference-count":82,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,3,31]],"date-time":"2020-03-31T00:00:00Z","timestamp":1585612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006280","name":"Ministerio de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["120356933228"],"award-info":[{"award-number":["120356933228"]}],"id":[{"id":"10.13039\/501100006280","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["RD16\/0027\/0008"],"award-info":[{"award-number":["RD16\/0027\/0008"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","award":["RTI2018-094434-B-I00"],"award-info":[{"award-number":["RTI2018-094434-B-I00"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The Tc964 protein was initially identified by its presence in the interactome associated with the LYT1 mRNAs, which code for a virulence factor of Trypanosoma cruzi. Tc964 is annotated in the T. cruzi genome as a hypothetical protein. According to phylogenetic analysis, the protein is conserved in the different genera of the Trypanosomatidae family; however, recognizable orthologues were not identified in other groups of organisms. Therefore, as a first step, an in-depth molecular characterization of the Tc946 protein was carried out. Based on structural predictions and molecular dynamics studies, the Tc964 protein would belong to a particular class of GTPases. Subcellular fractionation analysis indicated that Tc964 is a nucleocytoplasmic protein. Additionally, the protein was expressed as a recombinant protein in order to analyze its antigenicity with sera from Chagas disease (CD) patients. Tc964 was found to be antigenic, and B-cell epitopes were mapped by the use of synthetic peptides. In parallel, the Leishmania major homologue (Lm964) was also expressed as recombinant protein and used for a preliminary evaluation of antigen cross-reactivity in CD patients. Interestingly, Tc964 was recognized by sera from Chronic CD (CCD) patients at different stages of disease severity, but no reactivity against this protein was observed when sera from Colombian patients with cutaneous leishmaniasis were analyzed. Therefore, Tc964 would be adequate for CD diagnosis in areas where both infections (CD and leishmaniasis) coexist, even though additional assays using larger collections of sera are needed in order to confirm its usefulness for differential serodiagnosis.<\/jats:p>","DOI":"10.3390\/ijms21072432","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T03:44:13Z","timestamp":1585712653000},"page":"2432","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1949-4736","authenticated-orcid":false,"given":"Elizabeth","family":"Ruiz-M\u00e1rvez","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 # 40- 62, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar Augusto","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 # 40- 62, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5511-9402","authenticated-orcid":false,"given":"Eliana Roc\u00edo","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 # 40- 62, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5751-9773","authenticated-orcid":false,"given":"Magda Mellisa","family":"Fl\u00f3rez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Inmunotoxicolog\u00eda, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Carrera 30 # 45-01, Bogota, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2947-7796","authenticated-orcid":false,"given":"Gabriela","family":"Delgado","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Inmunotoxicolog\u00eda, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Carrera 30 # 45-01, Bogota, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7829-0568","authenticated-orcid":false,"given":"Fanny","family":"Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"N\u00facleo de Biotecnolog\u00eda Curauma (NBC), Pontificia Universidad Cat\u00f3lica de Valparaiso, Avenida Universidad, Curauma, Valparaiso 2373223, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3131-729X","authenticated-orcid":false,"given":"Paulino","family":"G\u00f3mez-Puertas","sequence":"additional","affiliation":[{"name":"Grupo de Modelado Molecular del Centro de Biolog\u00eda Molecular Severo Ochoa, Microbes in Health and Welfare Department, Universidad Aut\u00f3noma de Madrid (CBMSO, CSIC-UAM), 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7550-0198","authenticated-orcid":false,"given":"Jos\u00e9 Mar\u00eda","family":"Requena","sequence":"additional","affiliation":[{"name":"Grupo Regulaci\u00f3n de la Expresi\u00f3n G\u00e9nica en Leishmania del Centro de Biolog\u00eda Molecular Severo Ochoa, Molecular Biology Department, Universidad Aut\u00f3noma de Madrid (CBMSO, CSIC-UAM), 28049 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3449-3961","authenticated-orcid":false,"given":"Concepci\u00f3n J.","family":"Puerta","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Facultad de Ciencias, Pontificia Universidad Javeriana, Carrera 7 # 40- 62, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1590\/S0074-02761909000200008","article-title":"Nova tripanozomiaze humana","volume":"1","author":"Chagas","year":"1909","journal-title":"Mem. 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