{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:07:36Z","timestamp":1791828456579,"version":"4.3.4"},"reference-count":57,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A drug design strategy of carbonic anhydrase inhibitors (CAIs) belonging to sulfonamides incorporating ureidoethylaminobenzyl tails is presented. A variety of substitution patterns on the ring and the tails, located on para- or meta- positions with respect to the sulfonamide warheads were incorporated in the new compounds. Inhibition of human carbonic anhydrases (hCA) isoforms I, II, IX and XII, involving various pathologies, was assessed with the new compounds. Selective inhibitory profile towards hCA II was observed, the most active compounds being low nM inhibitors (KIs of 2.8\u20139.2 nM, respectively). Extensive X-ray crystallographic analysis of several sulfonamides in an adduct with hCA I allowed an in-depth understanding of their binding mode and to lay a detailed structure-activity relationship.<\/jats:p>","DOI":"10.3390\/ijms21072560","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T05:59:47Z","timestamp":1586325587000},"page":"2560","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Benzylaminoethyureido-Tailed Benzenesulfonamides: Design, Synthesis, Kinetic and X-ray Investigations on Human Carbonic Anhydrases"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6873-0953","authenticated-orcid":false,"given":"Majid","family":"Ali","sequence":"first","affiliation":[{"name":"Dipartimento Neurofarba, Universit\u00e0 degli Studi di Firenze, Sezione di Scienze Farmaceutiche, Polo Scientifico, Via Ugo Schiff 6, Sesto Fiorentino, 50019 Florence, Italy"},{"name":"Department of Biomedical Sciences, Universit\u00e0 di Padova, Via Ugo Bassi 58\/B, 35131 Padua, Italy"},{"name":"Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, KPK 22060, Islamabad 45550, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5010-0355","authenticated-orcid":false,"given":"Murat","family":"Bozdag","sequence":"additional","affiliation":[{"name":"Dipartimento Neurofarba, Universit\u00e0 degli Studi di Firenze, Sezione di Scienze Farmaceutiche, Polo Scientifico, Via Ugo Schiff 6, Sesto Fiorentino, 50019 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5557-2776","authenticated-orcid":false,"given":"Umar","family":"Farooq","sequence":"additional","affiliation":[{"name":"Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, KPK 22060, Islamabad 45550, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1470-7192","authenticated-orcid":false,"given":"Andrea","family":"Angeli","sequence":"additional","affiliation":[{"name":"Dipartimento Neurofarba, Universit\u00e0 degli Studi di Firenze, Sezione di Scienze Farmaceutiche, Polo Scientifico, Via Ugo Schiff 6, Sesto Fiorentino, 50019 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1141-6146","authenticated-orcid":false,"given":"Fabrizio","family":"Carta","sequence":"additional","affiliation":[{"name":"Dipartimento Neurofarba, Universit\u00e0 degli Studi di Firenze, Sezione di Scienze Farmaceutiche, Polo Scientifico, Via Ugo Schiff 6, Sesto Fiorentino, 50019 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Berto","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Universit\u00e0 di Padova, Via Ugo Bassi 58\/B, 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Zanotti","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Universit\u00e0 di Padova, Via Ugo Bassi 58\/B, 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-0323","authenticated-orcid":false,"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"Dipartimento Neurofarba, Universit\u00e0 degli Studi di Firenze, Sezione di Scienze Farmaceutiche, Polo Scientifico, Via Ugo Schiff 6, Sesto Fiorentino, 50019 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1080\/17460441.2019.1651289","article-title":"Advances in the structural annotation of human carbonic anhydrases and impact on future drug discovery","volume":"14","author":"Nocentini","year":"2019","journal-title":"Expert Opin Drug Discov."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1042\/BCJ20160115","article-title":"Structure and function of carbonic anhydrases","volume":"473","author":"Supuran","year":"2016","journal-title":"Biochem. 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