{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:01:27Z","timestamp":1790812887906,"version":"4.1.0"},"reference-count":58,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["K2009-52P-20943-03-2, 2014-2979, 2018-02470"],"award-info":[{"award-number":["K2009-52P-20943-03-2, 2014-2979, 2018-02470"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004348","name":"Stockholms L\u00e4ns Landsting","doi-asserted-by":"publisher","award":["SOF-project"],"award-info":[{"award-number":["SOF-project"]}],"id":[{"id":"10.13039\/501100004348","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Swedish Dental Society","award":["-"],"award-info":[{"award-number":["-"]}]},{"name":"Region \u00d6sterg\u00f6tland (ALF grant)","award":["LIO-700931"],"award-info":[{"award-number":["LIO-700931"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the last years, several attempts have been made to study specific biological markers of temporomandibular disorders (TMD). So far, no laboratory tests have been appropriately validated for the diagnosis and prognosis of these disorders. This study aimed to investigate the proteomic profile of the whole stimulated saliva of TMD myalgia patients in order to evaluate potential diagnostic and\/or prognostic salivary candidate proteins which could be useful for the management of TMD. Twenty patients diagnosed with TMD myalgia according to the validated Diagnostic Criteria for TMD (DC\/TMD) and 20 matched healthy pain-free controls were enrolled. Saliva samples were collected in the morning. Comparative proteomic analysis was performed with two-dimensional gel electrophoresis followed by identification with liquid chromatography\u2013tandem mass spectrometry. Statistical analysis of the quantitative proteomics data revealed that 20 proteins were significantly altered in patients compared to controls. Among these proteins, 12 showed significantly increased levels, and 8 showed significantly decreased levels in patients with TMD myalgia compared to controls. The identified proteins are involved in metabolic processes, immune response, and stress response. This proteomic study shows that the salivary protein profile can discriminate patients with TMD myalgia from healthy subjects, but the protein signature has no correlation with the clinical features of TMD myalgia. Additional studies are needed to validate our observations in additional sample sets and to continue assessing the utility of saliva as a suitable sample for studying processes related to TMD myalgia.<\/jats:p>","DOI":"10.3390\/ijms21072569","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T05:59:47Z","timestamp":1586325587000},"page":"2569","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Protein Signature in Saliva of Temporomandibular Disorders Myalgia"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2352-8070","authenticated-orcid":false,"given":"Hajer","family":"Jasim","sequence":"first","affiliation":[{"name":"Division of Oral Diagnostics &amp; Rehabilitation, Department of Dental Medicine, Karolinska Institutet and Scandinavian Center for Orofacial neuroscience (SCON), SE 14104 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4152-5439","authenticated-orcid":false,"given":"Malin","family":"Ernberg","sequence":"additional","affiliation":[{"name":"Division of Oral Diagnostics &amp; Rehabilitation, Department of Dental Medicine, Karolinska Institutet and Scandinavian Center for Orofacial neuroscience (SCON), SE 14104 Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Carlsson","sequence":"additional","affiliation":[{"name":"Pain and Rehabilitation Centre, and Department of Health, Medicine and Caring Sciences, Link\u00f6ping University, SE 581 83 Link\u00f6ping, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4316-1264","authenticated-orcid":false,"given":"Bj\u00f6rn","family":"Gerdle","sequence":"additional","affiliation":[{"name":"Pain and Rehabilitation Centre, and Department of Health, Medicine and Caring Sciences, Link\u00f6ping University, SE 581 83 Link\u00f6ping, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6396-5104","authenticated-orcid":false,"given":"Bijar","family":"Ghafouri","sequence":"additional","affiliation":[{"name":"Pain and Rehabilitation Centre, and Department of Health, Medicine and Caring Sciences, Link\u00f6ping University, SE 581 83 Link\u00f6ping, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"ref_1","first-page":"102","article-title":"A review of temporomandibular joint disease (TMJD). 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