{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:33:54Z","timestamp":1787625234071,"version":"build-2736575974"},"reference-count":57,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T00:00:00Z","timestamp":1586908800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Many Pasteurella multocida strains are carried as commensals, while some cause disease in animals and humans. Some type D strains cause atrophic rhinitis in pigs, where the causative agent is known to be the Pasteurella multocida toxin (PMT). PMT activates three families of G-proteins\u2014Gq\/11, G12\/13, and Gi\/o\u2014leading to cellular mitogenesis and other sequelae. The effects of PMT on whole animals in vivo have been investigated previously, but only at the level of organ-specific pathogenesis. We report here the first study to screen all the organs targeted by the toxin by using the QE antibody that recognizes only PMT-modified G-proteins. Under our experimental conditions, short-term treatment of PMT is shown to have multiple in vivo targets, demonstrating G-alpha protein modification, stimulation of proliferation markers and expression of active \u03b2-catenin in a tissue- and cell-specific manner. This highlights the usefulness of PMT as an important tool for dissecting the specific roles of different G-alpha proteins in vivo.<\/jats:p>","DOI":"10.3390\/ijms21082739","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T05:15:41Z","timestamp":1587014141000},"page":"2739","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["In Vivo Targets of Pasteurella Multocida Toxin"],"prefix":"10.3390","volume":"21","author":[{"given":"Arshiya","family":"Banu","sequence":"first","affiliation":[{"name":"Department of Microbiology, King\u2019s College London, Guy\u2019s Hospital, London SE1 9RT, UK"},{"name":"Centre for Craniofacial and Regenerative Biology, King\u2019s College London, Guy\u2019s Hospital, London SE1 9RT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1424-5071","authenticated-orcid":false,"given":"Alistair J.","family":"Lax","sequence":"additional","affiliation":[{"name":"Department of Microbiology, King\u2019s College London, Guy\u2019s Hospital, London SE1 9RT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5941-8132","authenticated-orcid":false,"given":"Agamemnon E.","family":"Grigoriadis","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology, King\u2019s College London, Guy\u2019s Hospital, London SE1 9RT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2006.00442.x","article-title":"Pasteurella multocida pathogenesis: 125 years after Pasteur","volume":"265","author":"Harper","year":"2006","journal-title":"FEMS Microbiol. 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