{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:47:10Z","timestamp":1787028430228,"version":"build-2736575974"},"reference-count":43,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T00:00:00Z","timestamp":1586995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In this work, we sought to investigate the effects of a thiosulfinate-enriched garlic extract, co-administered with 5-fluorouracil (5-FU) or oxaliplatin chemotherapy, on the viability of colon cancer cells (Caco-2 and HT-29). We also addressed the economic feasibility of a new combined treatment of this thiosulfinate-enriched garlic extract, with oxaliplatin that could reduce the dosage and costs of a monotherapy. The thiosulfinate-enriched garlic extract not only enhanced the impact of 5-FU and oxaliplatin (500 \u00b5M) in decreasing Caco-2 and HT-29 viability, but also showed a higher effect than standard 5-FU and oxaliplatin chemotherapy as anti-cancer agents. These results provided evidences for the combination of lyophilized garlic extract and 5-FU or oxaliplatin as a novel chemotherapy regimen in colon cancer cells that may also reduce the clinical therapy costs.<\/jats:p>","DOI":"10.3390\/ijms21082766","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T13:01:39Z","timestamp":1587042099000},"page":"2766","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Cost Effective Use of a Thiosulfinate-Enriched Allium sativum Extract in Combination with Chemotherapy in Colon Cancer"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3588-6273","authenticated-orcid":false,"given":"Jose Manuel","family":"Perez-Ortiz","sequence":"first","affiliation":[{"name":"Translational Research Unit, Hospital General Universitario de Ciudad Real, 13004 Ciudad Real, Spain"},{"name":"Department of Medical Sciences, School of Medicine, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5758-7592","authenticated-orcid":false,"given":"Eva Maria","family":"Galan-Moya","sequence":"additional","affiliation":[{"name":"Translational Research Unit, Hospital General Universitario de Ciudad Real, 13004 Ciudad Real, Spain"},{"name":"Translational Oncology Laboratory, Centro Regional de Investigaciones Biom\u00e9dicas (CRIB), Universidad de Castilla-La Mancha, 02008 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel Angel","family":"de la Cruz-Morcillo","sequence":"additional","affiliation":[{"name":"Translational Research Unit, Hospital General Universitario de Ciudad Real, 13004 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8838-1976","authenticated-orcid":false,"given":"Juan Francisco","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Institute of Chemical and Environmental Technology, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Gracia","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Institute of Chemical and Environmental Technology, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8541-4285","authenticated-orcid":false,"given":"Maria Teresa","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Institute of Chemical and Environmental Technology, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Javier","family":"Redondo-Calvo","sequence":"additional","affiliation":[{"name":"Translational Research Unit, Hospital General Universitario de Ciudad Real, 13004 Ciudad Real, Spain"},{"name":"Department of Medical Sciences, School of Medicine, Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain"},{"name":"Department of Anesthesiology, Hospital General Universitario de Ciudad Real, 13004 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.nbt.2018.08.005","article-title":"The growing U.S. bioeconomy: Drivers, development and constraints","volume":"49","author":"Guo","year":"2019","journal-title":"New Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"303","DOI":"10.3390\/metabo2020303","article-title":"A historical overview of natural products in drug discovery","volume":"2","author":"Dias","year":"2012","journal-title":"Metabolites"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/j.1747-0285.2009.00787.x","article-title":"Natural products-Based drugs and membrane permeability, a hypothesis","volume":"73","author":"John","year":"2009","journal-title":"Chem. 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