{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:09:16Z","timestamp":1782122956113,"version":"3.54.5"},"reference-count":70,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["18-15-00043"],"award-info":[{"award-number":["18-15-00043"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004191","name":"Novo Nordisk","doi-asserted-by":"publisher","award":["NNF19OC0056371"],"award-info":[{"award-number":["NNF19OC0056371"]}],"id":[{"id":"10.13039\/501100004191","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004191","name":"Novo Nordisk","doi-asserted-by":"publisher","award":["NNF18OC0052021"],"award-info":[{"award-number":["NNF18OC0052021"]}],"id":[{"id":"10.13039\/501100004191","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>While the role of circulating ouabain-like compounds in the cardiovascular and central nervous systems, kidney and other tissues in health and disease is well documented, little is known about its effects in skeletal muscle. In this study, rats were intraperitoneally injected with ouabain (0.1\u201310 \u00b5g\/kg for 4 days) alone or with subsequent injections of lipopolysaccharide (1 mg\/kg). Some rats were also subjected to disuse for 6 h by hindlimb suspension. In the diaphragm muscle, chronic ouabain (1 \u00b5g\/kg) hyperpolarized resting potential of extrajunctional membrane due to specific increase in electrogenic transport activity of the \u03b12 Na,K-ATPase isozyme and without changes in \u03b11 and \u03b12 Na,K-ATPase protein content. Ouabain (10\u201320 nM), acutely applied to isolated intact diaphragm muscle from not injected rats, hyperpolarized the membrane to a similar extent. Chronic ouabain administration prevented lipopolysaccharide-induced (diaphragm muscle) or disuse-induced (soleus muscle) depolarization of the extrajunctional membrane. No stimulation of the \u03b11 Na,K-ATPase activity in human red blood cells, purified lamb kidney and Torpedo membrane preparations by low ouabain concentrations was observed. Our results suggest that skeletal muscle electrogenesis is subjected to regulation by circulating ouabain via the \u03b12 Na,K-ATPase isozyme that could be important for adaptation of this tissue to functional impairment.<\/jats:p>","DOI":"10.3390\/ijms21082875","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T04:49:38Z","timestamp":1587444578000},"page":"2875","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain"],"prefix":"10.3390","volume":"21","author":[{"given":"Violetta V.","family":"Kravtsova","sequence":"first","affiliation":[{"name":"Department of General Physiology, St. Petersburg State University, St. Petersburg 199034, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Bouzinova","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, University of Aarhus, C 8000 Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3303-1095","authenticated-orcid":false,"given":"Vladimir V.","family":"Matchkov","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, University of Aarhus, C 8000 Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2690-9226","authenticated-orcid":false,"given":"Igor I.","family":"Krivoi","sequence":"additional","affiliation":[{"name":"Department of General Physiology, St. Petersburg State University, St. Petersburg 199034, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1085\/jgp.201310980","article-title":"Quantification of Na+,K+ pumps and their transport rate in skeletal muscle: Functional significance","volume":"142","author":"Clausen","year":"2013","journal-title":"J. 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