{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:51:47Z","timestamp":1787316707536,"version":"build-2736575974"},"reference-count":27,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T00:00:00Z","timestamp":1588118400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hallym University Research Fund","award":["2019"],"award-info":[{"award-number":["2019"]}]},{"DOI":"10.13039\/501100003665","name":"National IT Industry Promotion Agency","doi-asserted-by":"publisher","award":["R-20190315-004075"],"award-info":[{"award-number":["R-20190315-004075"]}],"id":[{"id":"10.13039\/501100003665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Little is known of the adaptive immune response to subarachnoid hemorrhage (SAH). This study was the first to investigate whether T cell receptor (TCR) immune repertoire may provide a better understanding of T cell immunology in delayed cerebral ischemia (DCI). We serially collected peripheral blood in five SAH patients with DCI. High-throughput sequencing was used to analyze the TCR \u03b2 chain (TCRB) complimentary determining regions (CDR) 3 repertoire. We evaluated the compositions and variations of the repertoire between admission and the DCI period, for severe DCI and non-severe DCI patients. Clonality did not differ significantly between admission and DCI. Severe DCI patients had significantly lower clonality than non-severe DCI patients (p value = 0.019). A read frequency of 0.005% \u2264 \u2013 &lt; 0.05% dominated the clonal expansion in non-severe DCI patients. Regarding repertoire diversity, severe DCI had a higher diversity score on admission than non-severe DCI. The CDR3 lengths were similar between admission and DCI. Among 728 annotated V-J gene pairs, we found that the relative frequencies of two V-J pairs were different at the occurrence of DCI than at admission, with T cells increasing by over 15%. TCRB CDR3 repertoires may serve as biomarkers to identify severe DCI patients.<\/jats:p>","DOI":"10.3390\/ijms21093149","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T13:23:45Z","timestamp":1588166625000},"page":"3149","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of the TCR \u03b2 Chain CDR3 Repertoire in Subarachnoid Hemorrhage Patients with Delayed Cerebral Ischemia"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3374-5554","authenticated-orcid":false,"given":"Bong Jun","family":"Kim","sequence":"first","affiliation":[{"name":"Institute of New Frontier Stroke Research, Hallym University College of Medicine, Chuncheon 24253, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Hyuk","family":"Youn","sequence":"additional","affiliation":[{"name":"Institute of New Frontier Stroke Research, Hallym University College of Medicine, Chuncheon 24253, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngmi","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of New Frontier Stroke Research, Hallym University College of Medicine, Chuncheon 24253, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8543-6855","authenticated-orcid":false,"given":"Jin Pyeong","family":"Jeon","sequence":"additional","affiliation":[{"name":"Institute of New Frontier Stroke Research, Hallym University College of Medicine, Chuncheon 24253, Korea"},{"name":"Genetic and Research Inc., Chuncheon 24253, Korea"},{"name":"Department of Neurosurgery, Hallym University College of Medicine, Chuncheon 24253, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s12967-019-1788-4","article-title":"Peripheral T cell receptor \u03b2 immune repertoire is promptly reconstituted after acute myocardial infarction","volume":"17","author":"Li","year":"2019","journal-title":"J. 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