{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T19:52:40Z","timestamp":1766087560873,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T00:00:00Z","timestamp":1589155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["20930"],"award-info":[{"award-number":["20930"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]},{"name":"SIE-Societ\u00e0 Italiana di Ematologia- e Associazione \u201cAmici di Beat Leukemia Dr. Alessandro Cevenini ONLUS","award":["\/"],"award-info":[{"award-number":["\/"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Growing evidence highlights the endocannabinoid (EC) system involvement in cancer progression. Lipid mediators of this system are secreted by hematopoietic cells, including the ECs 2-arachidonoyl-glycerol (2AG) and arachidonoyl-ethanolamide (AEA), the 2AG metabolite 1AG, and members of N-acylethanolamine (NAE) family\u2014palmitoyl-ethanolamide (PEA) and oleoyl-ethanolamide (OEA). However, the relevance of the EC system in myeloproliferative neoplasms (MPN) was never investigated. We explored the EC plasma profile in 55 MPN patients, including myelofibrosis (MF; n = 41), polycythemia vera (PV; n = 9), and essential thrombocythemia (ET; n = 5) subclasses and in 10 healthy controls (HC). AEA, PEA, OEA, 2AG, and 1AG plasma levels were measured by LC\u2013MS\/MS. Overall considered, MPN patients displayed similar EC and NAE levels compared to HC. Nonetheless, AEA levels in MPN were directly associated with the platelet count. MF patients showed higher levels of the sum of 2AG and 1AG compared to ET and PV patients, higher OEA\/AEA ratios compared to HC and ET patients, and higher OEA\/PEA ratios compared to HC. Furthermore, the sum of 2AG and 1AG positively correlated with JAK2V617F variant allele frequency and splenomegaly in MF and was elevated in high-risk PV patients compared to in low-risk PV patients. In conclusion, our work revealed specific alterations of ECs and NAE plasma profile in MPN subclasses and potentially relevant associations with disease severity.<\/jats:p>","DOI":"10.3390\/ijms21093399","type":"journal-article","created":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T12:26:30Z","timestamp":1589199990000},"page":"3399","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Disease-Specific Derangement of Circulating Endocannabinoids and N-Acylethanolamines in Myeloproliferative Neoplasms"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8443-605X","authenticated-orcid":false,"given":"Dorian","family":"Forte","sequence":"first","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flaminia","family":"Fanelli","sequence":"additional","affiliation":[{"name":"Unit of Endocrinology and Prevention and Care of Diabetes, Center for Applied Biomedical Research, Department of Medical and Surgical Sciences, University of Bologna, University-Hospital S.Orsola, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Mezzullo","sequence":"additional","affiliation":[{"name":"Unit of Endocrinology and Prevention and Care of Diabetes, Center for Applied Biomedical Research, Department of Medical and Surgical Sciences, University of Bologna, University-Hospital S.Orsola, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Barone","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Corradi","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Auteri","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Bartoletti","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Martello","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuela","family":"Ottaviani","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Oncology and Hematology, University-Hospital S.Orsola, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Terragna","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Curti","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Oncology and Hematology, University-Hospital S.Orsola, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4182-3110","authenticated-orcid":false,"given":"Uberto","family":"Pagotto","sequence":"additional","affiliation":[{"name":"Unit of Endocrinology and Prevention and Care of Diabetes, Center for Applied Biomedical Research, Department of Medical and Surgical Sciences, University of Bologna, University-Hospital S.Orsola, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Palandri","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Oncology and Hematology, University-Hospital S.Orsola, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Cavo","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Catani","sequence":"additional","affiliation":[{"name":"Institute of Hematology \u201cL. e A. Ser\u00e0gnoli\u201d, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), School of Medicine, University of Bologna, 40138 Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.irn.2015.10.001","article-title":"The Endocannabinoid Signaling System in the CNS: A Primer","volume":"125","author":"Hillard","year":"2015","journal-title":"Int. Rev. Neurobiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.ynstr.2015.05.001","article-title":"Circulating endocannabinoids during hematopoietic stem cell transplantation: A pilot study","volume":"2","author":"Knight","year":"2015","journal-title":"Neurobiol. Stress."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s41232-018-0086-5","article-title":"Endocannabinoids and related N-acylethanolamines: Biological activities and metabolism","volume":"38","author":"Tsuboi","year":"2018","journal-title":"Inflamm. Regen."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.2174\/092986710790980023","article-title":"CB(1) cannabinoid receptors and their associated proteins","volume":"17","author":"Howlett","year":"2010","journal-title":"Curr. Med. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"839403","DOI":"10.1155\/2015\/839403","article-title":"Clinical Significance of Cannabinoid Receptors CB1 and CB2 Expression in Human Malignant and Benign Thyroid Lesions","volume":"2015","author":"Lakiotaki","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.exphem.2017.09.009","article-title":"Human bone marrow mesenchymal stem cells secrete endocannabinoids that stimulate in vitro hematopoietic stem cell migration effectively comparable to beta-adrenergic stimulation","volume":"57","author":"Kose","year":"2018","journal-title":"Exp. Hematol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1194\/jlr.M021378","article-title":"Estimation of reference intervals of five endocannabinoids and endocannabinoid related compounds in human plasma by two dimensional- LC\/MS\/MS","volume":"53","author":"Fanelli","year":"2012","journal-title":"J. Lipid Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.molmet.2017.03.005","article-title":"Plasma 2-arachidonoylglycerol is a biomarker of age and menopause related insulin resistance and dyslipidemia in lean but not in obese men and women","volume":"6","author":"Fanelli","year":"2017","journal-title":"Mol. Metab."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"39","DOI":"10.3389\/fncel.2017.00039","article-title":"The Ratio of 2-AG to Its Isomer 1-AG as an Intrinsic Fine Tuning Mechanism of CB1 Receptor Activation","volume":"11","author":"Docs","year":"2017","journal-title":"Front Cell Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.ab.2012.11.008","article-title":"Liquid chromatography-tandem mass spectrometry analysis of free and esterified fatty acid N-acyl ethanolamines in plasma and blood cells","volume":"434","author":"Balvers","year":"2013","journal-title":"Anal. Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/bph.12907","article-title":"Palmitoylethanolamide, a naturally occurring lipid, is an orally effective intestinal anti-inflammatory agent","volume":"172","author":"Borrelli","year":"2015","journal-title":"Br. J. Pharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1210\/jc.2010-2028","article-title":"Central and peripheral endocannabinoids and cognate acylethanolamides in humans: Association with race, adiposity, and energy expenditure","volume":"96","author":"Jumpertz","year":"2011","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.molmet.2018.06.002","article-title":"Profiling plasma N-Acylethanolamine levels and their ratios as a biomarker of obesity and dysmetabolism","volume":"14","author":"Fanelli","year":"2018","journal-title":"Mol. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1136\/gutjnl-2013-305005","article-title":"Palmitoylethanolamide improves colon inflammation through an enteric glia\/toll like receptor 4-dependent PPAR-alpha activation","volume":"63","author":"Esposito","year":"2014","journal-title":"Gut"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3938","DOI":"10.4161\/15384101.2014.982941","article-title":"2-Arachidonoylglycerol enhances platelet formation from human megakaryoblasts","volume":"13","author":"Gasperi","year":"2014","journal-title":"Cell Cycle."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.1017\/S1462399409000957","article-title":"Emerging role of the cannabinoid receptor CB2 in immune regulation: Therapeutic prospects for neuroinflammation","volume":"11","author":"Cabral","year":"2009","journal-title":"Expert Rev. Mol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/imm.12441","article-title":"Endocannabinoid signalling in innate and adaptive immunity","volume":"144","author":"Chiurchiu","year":"2015","journal-title":"Immunology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1096\/fj.201500132","article-title":"Oleoylethanolamine and palmitoylethanolamine modulate intestinal permeability in vitro via TRPV1 and PPARalpha","volume":"31","author":"Karwad","year":"2017","journal-title":"Faseb J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5716","DOI":"10.1096\/fj.201800107R","article-title":"Bioactive lipids ALIAmides differentially modulate inflammatory responses of distinct subsets of primary human T lymphocytes","volume":"32","author":"Chiurchiu","year":"2018","journal-title":"Faseb J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"65s","DOI":"10.1111\/j.1537-2995.2011.03368.x","article-title":"Regulation of hematopoietic stem cell trafficking and mobilization by the endocannabinoid system","volume":"51","author":"Jiang","year":"2011","journal-title":"Transfusion"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejphar.2008.05.002","article-title":"Endocannabinoids as positive or negative factors in hematopoietic cell migration and differentiation","volume":"595","author":"Patinkin","year":"2008","journal-title":"Eur. J. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.plipres.2013.05.004","article-title":"Cannabinoid receptor signaling in progenitor\/stem cell proliferation and differentiation","volume":"52","author":"Chiurchiu","year":"2013","journal-title":"Prog. Lipid Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1002\/ijc.30483","article-title":"Cannabinoid derivatives exert a potent anti-myeloma activity both in vitro and in vivo","volume":"140","author":"Barbado","year":"2017","journal-title":"Int. J. Cancer"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/sj.bjp.0707420","article-title":"Endocannabinoids and the haematological system","volume":"152","author":"Randall","year":"2007","journal-title":"Br. J. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2350","DOI":"10.2174\/0929867323666160530144934","article-title":"Endocannabinoid System: A Promising Therapeutic Target for the Treatment of Haematological Malignancies?","volume":"23","author":"Giaginis","year":"2016","journal-title":"Curr. Med. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/S0301-472X(01)00779-2","article-title":"Leukemic predisposition of pSca-1\/Cb2 transgenic mice","volume":"30","author":"Joosten","year":"2002","journal-title":"Exp. Hematol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1182\/blood-2003-12-4357","article-title":"The peripheral cannabinoid receptor Cb2, frequently expressed on AML blasts, either induces a neutrophilic differentiation block or confers abnormal migration properties in a ligand-dependent manner","volume":"104","author":"Jorda","year":"2004","journal-title":"Blood"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1749-6632.2003.tb03227.x","article-title":"Identification, characterization, and function of a novel oncogene: The peripheral cannabinoid receptor Cb2","volume":"996","author":"Jorda","year":"2003","journal-title":"Ann. N. Y. Acad. Sci."},{"doi-asserted-by":"crossref","unstructured":"Kampa-Schittenhelm, K.M., Salitzky, O., Akmut, F., Illing, B., Kanz, L., Salih, H.R., and Schittenhelm, M.M. (2016). Dronabinol has preferential antileukemic activity in acute lymphoblastic and myeloid leukemia with lymphoid differentiation patterns. BMC Cancer, 16.","key":"ref_29","DOI":"10.1186\/s12885-015-2029-8"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1200\/JCO.2010.29.8711","article-title":"Myeloproliferative Neoplasms: Molecular Pathophysiology, Essential Clinical Understanding, and Treatment Strategies","volume":"29","author":"Tefferi","year":"2011","journal-title":"J. Clin. Oncol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1002\/cncr.32664","article-title":"Life after ruxolitinib: Reasons for discontinuation, impact of disease phase, and outcomes in 218 patients with myelofibrosis","volume":"126","author":"Palandri","year":"2020","journal-title":"Cancer"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1182\/blood-2009-09-245837","article-title":"A dynamic prognostic model to predict survival in primary myelofibrosis: A study by the IWG-MRT (International Working Group for Myeloproliferative Neoplasms Research and Treatment)","volume":"115","author":"Passamonti","year":"2009","journal-title":"Blood"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"339","DOI":"10.3389\/fphar.2019.00339","article-title":"The Endocannabinoid System as a Target in Cancer Diseases: Are We There Yet?","volume":"10","author":"Moreno","year":"2019","journal-title":"Front Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"272","DOI":"10.18632\/oncoscience.33","article-title":"Regulation of circulating endocannabinoids associated with cancer and metastases in mice and humans","volume":"1","author":"Sailler","year":"2014","journal-title":"Oncoscience"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"43974","DOI":"10.18632\/oncotarget.9949","article-title":"Crucial factors of the inflammatory microenvironment (IL-1beta\/TNF-alpha\/TIMP-1) promote the maintenance of the malignant hemopoietic clone of myelofibrosis: An in vitro study","volume":"7","author":"Sollazzo","year":"2016","journal-title":"Oncotarget"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s00018-009-0198-9","article-title":"Anandamide extends platelets survival through CB(1)-dependent Akt signaling","volume":"67","author":"Catani","year":"2010","journal-title":"Cell Mol. Life Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.2337\/db06-0812","article-title":"Dysregulation of the peripheral and adipose tissue endocannabinoid system in human abdominal obesity","volume":"55","author":"Bluher","year":"2006","journal-title":"Diabetes"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1189\/jlb.3RU0115-021R","article-title":"Regulation of inflammation by cannabinoids, the endocannabinoids 2-arachidonoyl-glycerol and arachidonoyl-ethanolamide, and their metabolites","volume":"97","author":"Turcotte","year":"2015","journal-title":"J. Leukoc. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4449","DOI":"10.1007\/s00018-016-2300-4","article-title":"The CB2 receptor and its role as a regulator of inflammation","volume":"73","author":"Turcotte","year":"2016","journal-title":"Cell Mol. Life Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/npp.2017.130","article-title":"Circulating Endocannabinoids: From Whence Do They Come and Where are They Going?","volume":"43","author":"Hillard","year":"2018","journal-title":"Neuropsychopharmacology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5860657","DOI":"10.1155\/2016\/5860657","article-title":"Circulating Calreticulin Is Increased in Myelofibrosis: Correlation with Interleukin-6 Plasma Levels, Bone Marrow Fibrosis, and Splenomegaly","volume":"2016","author":"Sollazzo","year":"2016","journal-title":"Mediat. Inflamm."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1182\/blood-2006-12-064626","article-title":"Pivotal contributions of megakaryocytes to the biology of idiopathic myelofibrosis","volume":"110","author":"Ciurea","year":"2007","journal-title":"Blood"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e2018068","DOI":"10.4084\/mjhid.2018.068","article-title":"Megakaryocyte Contribution to Bone Marrow Fibrosis: Many Arrows in the Quiver","volume":"10","author":"Malara","year":"2018","journal-title":"Mediterr. J. Hematol. Infect Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.ccell.2018.08.016","article-title":"Subversion of Systemic Glucose Metabolism as a Mechanism to Support the Growth of Leukemia Cells","volume":"34","author":"Ye","year":"2018","journal-title":"Cancer Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1096\/fj.01-0181com","article-title":"Activation of PAF receptors results in enhanced synthesis of 2-arachidonoylglycerol (2-AG) in immune cells","volume":"15","author":"Berdyshev","year":"2001","journal-title":"Faseb J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e1345402","DOI":"10.1080\/2162402X.2017.1345402","article-title":"Mutations in JAK2 and Calreticulin genes are associated with specific alterations of the immune system in myelofibrosis","volume":"6","author":"Romano","year":"2017","journal-title":"Oncoimmunology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/284706","article-title":"Impact of Inflammation on Myeloproliferative Neoplasm Symptom Development","volume":"2015","author":"Geyer","year":"2015","journal-title":"Mediat. Inflamm."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3324\/haematol.2016.149559","article-title":"Associations between gender, disease features and symptom burden in patients with myeloproliferative neoplasms: An analysis by the MPN QOL International Working Group","volume":"102","author":"Geyer","year":"2017","journal-title":"Haematologica"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/s41408-018-0128-x","article-title":"Gender effect on phenotype and genotype in patients with post- polycythemia vera and post-essential thrombocythemia myelofibrosis: Results from the MYSEC project","volume":"8","author":"Barraco","year":"2018","journal-title":"Blood Cancer J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1007\/s00018-015-1924-0","article-title":"Downstream effects of endocannabinoid on blood cells: Implications for health and disease","volume":"72","author":"Gasperi","year":"2015","journal-title":"Cell Mol. Life Sci."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/9\/3399\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:27:47Z","timestamp":1760174867000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/9\/3399"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,11]]},"references-count":50,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["ijms21093399"],"URL":"https:\/\/doi.org\/10.3390\/ijms21093399","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2020,5,11]]}}}