{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:40:17Z","timestamp":1788925217447,"version":"build-2803163510"},"reference-count":67,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T00:00:00Z","timestamp":1589760000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["2010ARBLT7_005"],"award-info":[{"award-number":["2010ARBLT7_005"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Carbonic anhydrase (CA) is a widespread metalloenzyme playing a pivotal role in several physiological processes. Many studies have demonstrated the in vitro and in vivo sensitivity of CA to the exposure to several classes of pesticides in both humans and wildlife. This review aims to analyze and to discuss the literature available in this field, providing a comprehensive view useful to foresee perspectives for the development of novel CA-based pesticide biomarkers. The analysis of the available data highlighted the ability of several pesticide molecules to interact directly with the enzyme in humans and wildlife and to inhibit CA activity in vitro and in vivo, with possible alterations of key physiological functions. The analysis disclosed key areas of further research and, at the same time, identified some perspectives for the development of novel CA-based sensitive biomarkers to pesticide exposure, suitable to be used in several fields from human biomonitoring in occupational and environmental medicine to environmental monitoring on non-target species.<\/jats:p>","DOI":"10.3390\/ijms21103562","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T11:34:14Z","timestamp":1589801654000},"page":"3562","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Carbonic Anhydrase Sensitivity to Pesticides: Perspectives for Biomarker Development"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3752-0192","authenticated-orcid":false,"given":"Maria Giulia","family":"Lionetto","sequence":"first","affiliation":[{"name":"Dept. of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Via prov.le Lecce-Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7928-4573","authenticated-orcid":false,"given":"Roberto","family":"Caricato","sequence":"additional","affiliation":[{"name":"Dept. of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Via prov.le Lecce-Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elena","family":"Giordano","sequence":"additional","affiliation":[{"name":"Dept. of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Via prov.le Lecce-Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1042\/BCJ20160115","article-title":"Structure and function of carbonic anhydrases","volume":"473","author":"Supuran","year":"2016","journal-title":"Biochem. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.plantsci.2017.12.002","article-title":"The many types of carbonic anhydrases in photosynthetic organisms","volume":"268","author":"DiMario","year":"2018","journal-title":"Plant Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1080\/14756366.2020.1755852","article-title":"Bacterial \u03b9-carbonic anhydrase: A new active class of carbonic anhydrase identified in the genome of the Gram-negative bacterium Burkholderia territorii","volume":"35","author":"Nocentini","year":"2020","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lionetto, M.G., Caricato, R., Giordano, M.E., and Schettino, T. (2016). The complex relationship between metals and carbonic anhydrase: New insights and perspectives. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17010127"},{"key":"ref_5","first-page":"1","article-title":"Protective role of carbonic anhydrases III and VII in cellular defense mechanisms upon redox unbalance","volume":"2018","author":"Monti","year":"2018","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"345","DOI":"10.3109\/14756366.2015.1122001","article-title":"How many carbonic anhydrase inhibition mechanisms exist?","volume":"31","author":"Supuran","year":"2016","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4336","DOI":"10.1016\/j.bmc.2007.04.020","article-title":"Carbonic anhydrases as targets for medicinal chemistry","volume":"15","author":"Supuran","year":"2007","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3467","DOI":"10.1016\/j.bmcl.2010.05.009","article-title":"Carbonic anhydrase inhibitors","volume":"15","author":"Supuran","year":"2010","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.jes.2016.11.012","article-title":"Pesticide exposures and respiratory health in general populations","volume":"51","author":"Ye","year":"2017","journal-title":"J. Environ. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1126\/science.1237227","article-title":"Current Challenges and Trends in the Discovery of Agrochemicals","volume":"341","author":"Lamberth","year":"2013","journal-title":"Science"},{"key":"ref_11","first-page":"159","article-title":"Literature review on epidemiological studies linking exposure to pesticides and health effects","volume":"EN-497","author":"Ntzani","year":"2013","journal-title":"EFSA"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Stanley, J., and Preetha, G. (2016). Pesticide Toxicity to Non-Target Organisms: Exposure, Toxicity and Risk Assessment Methodologies, Springer.","DOI":"10.1007\/978-94-017-7752-0"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Stoytcheva, M. (2011). Pesticide biomarkers. Pesticides in the Modern World\u2014Pests Control and Pesticides Exposure and Toxicity Assessment, InTech.","DOI":"10.5772\/948"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Stoytcheva, M. (2011). Biological markers of human exposure to pesticides. Pesticides in the Modern World\u2014Pests Control and Pesticides Exposure and Toxicity Assessment, InTech.","DOI":"10.5772\/948"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/321213","article-title":"Acetylcholinesterase as a biomarker in Environmental and occupational medicine: New insights and future perspectives","volume":"2013","author":"Lionetto","year":"2013","journal-title":"BioMed Res. Intern."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2174\/1875318301909010001","article-title":"Pollution Biomarkers in Environmental and Human Biomonitoring","volume":"9","author":"Lionetto","year":"2019","journal-title":"Open Biomark. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.3390\/ijerph9113965","article-title":"Carbonic anhydrase as pollution biomarker: An ancient enzyme with a new use","volume":"9","author":"Lionetto","year":"2012","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4475","DOI":"10.3892\/mmr.2016.5817","article-title":"Occupational exposure to pesticides as a possible risk factor for the development of chronic diseases in humans","volume":"14","author":"Gangemi","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1139\/o91-116","article-title":"Why are there carbonic anhydrases in the liver?","volume":"69","author":"Dodgson","year":"1991","journal-title":"Biochem. Cell. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"77","DOI":"10.3109\/2000-1967-131","article-title":"Carbonic Anhydrase III in Liver and Muscle of Male Rats Purification and Properties","volume":"107","author":"Wistrand","year":"2009","journal-title":"Upsala J. Med Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.resp.2012.06.001","article-title":"New insights into the many functions of carbonic anhydrase in fish gills","volume":"184","author":"Gilmour","year":"2012","journal-title":"Respir. Physiol. Neurobiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.pestbp.2010.01.003","article-title":"In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills","volume":"97","author":"Ceyhun","year":"2010","journal-title":"Pestic. Biochem. Phys."},{"key":"ref_23","first-page":"171","article-title":"The in vitro effects of some pesticides on carbonic anhydrase activity of Oncorhynchus mykiss and Cyprinus carpio carpio fish","volume":"A132","year":"2006","journal-title":"J. Hazard. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.etap.2012.04.007","article-title":"Influence of pesticides on the pH regulatory enzyme, carbonic anhydrase, from European Seabass liver and bovine erythrocytes","volume":"34","author":"Yerlikaya","year":"2012","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1177\/0748233713475508","article-title":"Influence of pesticide exposure on carbonic anhydrase II from sheep stomach","volume":"31","author":"Isgor","year":"2015","journal-title":"Toxicol. Ind. Health"},{"key":"ref_26","first-page":"25","article-title":"Differential in vitro effects of some pesticides on carbonic anhydrase activities from some freshwater and seawater fish erythrocytes","volume":"13","author":"Guler","year":"2004","journal-title":"Fresen. Environ. Bull."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.pestbp.2007.01.006","article-title":"In vitro inhibition of the carbonic anhydrase from saanen goat (Capra hircus) with pesticides","volume":"88","author":"Sinan","year":"2007","journal-title":"Pestic. Biochem. Phys."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"895","DOI":"10.3109\/14756366.2011.554415","article-title":"Characterization and inhibition studies of an \u03b1-carbonic anhydrase from the endangered sturgeon species Acipenser gueldenstaedti","volume":"26","author":"Kolayli","year":"2011","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_29","first-page":"13473","article-title":"Inhibitory effects of some pesticides and metals on carbonic anhydrase purified from shabut fish (Barbus grypus) gill tissue","volume":"17","author":"Yerlinkaya","year":"2019","journal-title":"Appl. Ecol. Env. Res."},{"key":"ref_30","first-page":"549","article-title":"In vitro effects of some herbicides and fungicides on human erythrocyte carbonic anhydrase activity","volume":"21","author":"Demir","year":"2012","journal-title":"Fresen. Environ. Bull."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1080\/14756366.2016.1232255","article-title":"Carbonic anhydrase from Apis mellifera: Purification and inhibition by pesticides","volume":"32","author":"Soydan","year":"2017","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1080\/03067310500154320","article-title":"Carbonic anhydrase-based environmental bioassay","volume":"85","author":"Lionetto","year":"2005","journal-title":"Int. J. Envir. Anal. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.pestbp.2009.12.006","article-title":"Risk assessment of pesticides and fungicides for acid\u2013base regulation and salt transport in rainbow trout tissues","volume":"97","author":"Ekinci","year":"2010","journal-title":"Pest. Biochem. Physiol."},{"key":"ref_34","first-page":"108605","article-title":"Purification and characterization of the carbonic anhydrase enzyme from horse mackerel (Trachurus trachurus) muscle and the impact of some metal ions and pesticides on enzyme activity","volume":"226","author":"Caglayan","year":"2019","journal-title":"Comp. Biochem. Physiol. C."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"10607","DOI":"10.1007\/s11356-020-07611-z","article-title":"Inhibition effects of some pesticides and heavy metals on carbonic anhydrase enzyme activity purified from horse mackerel (Trachurus trachurus) gill tissues","volume":"27","author":"Caglayan","year":"2020","journal-title":"Environ. Sci. Pollut. R."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"773","DOI":"10.3109\/14756366.2015.1064406","article-title":"The effects of some avermectins on bovine carbonic anhydrase enzyme","volume":"31","author":"Kose","year":"2016","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e21894","DOI":"10.1002\/jbt.21894","article-title":"Herbicide oryzalin inhibits human carbonic anhydrases in vitro","volume":"31","author":"Baranauskiene","year":"2017","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/arch.10112","article-title":"Chloride channels as tools for developing selective insecticides","volume":"54","author":"Bloomquist","year":"2003","journal-title":"Arch Insect Biochem Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.exppara.2016.04.017","article-title":"Anthelmintic effect of herbicidal dinitroanilines on the nematode model Caenorhabditis elegans","volume":"167","author":"Vommaro","year":"2016","journal-title":"Exp Parasitol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1021\/jm300031j","article-title":"Dithiocarbamates Strongly Inhibit Carbonic Anhydrases and Show Antiglaucoma Action in Vivo","volume":"55","author":"Carta","year":"2012","journal-title":"J. Med. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2368","DOI":"10.1016\/j.bmc.2015.03.068","article-title":"Synthesis of a new series of dithiocarbamates with effective human carbonic anhydrase inhibitory activity and antiglaucoma action","volume":"23","author":"Bozdag","year":"2015","journal-title":"Bioorg. Med. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1016\/j.bmcl.2007.01.113","article-title":"Phosph(on)ate as a zinc-binding group in metalloenzyme inhibitors: X-ray crystal structure of the antiviral drug foscarnet complexed to human carbonic anhydrase I","volume":"17","author":"Temperini","year":"2007","journal-title":"Bioorganic Med. Chem. Lett."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Scozzafava, A., and Conway, J. (2004). Carbonic anhydrase activators. Carbonic Anhydrase\u2014Its Inhibitors and Activators, Supuran, C.T., CRC Press.","DOI":"10.1201\/9780203475300"},{"key":"ref_44","first-page":"929","article-title":"Parathion and salinity effects on gills and mesonephros carbonic anhydrase activity of the fish Oreochromis hornorum","volume":"49","author":"Beatriz","year":"1992","journal-title":"B Environ. Contam. Toxicol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"377","DOI":"10.2478\/10004-1254-65-2014-2547","article-title":"In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout","volume":"65","author":"Topal","year":"2014","journal-title":"Arh. Hig. Rada Toksikol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.chemosphere.2017.04.018","article-title":"An approach to clarify the effect mechanism of glyphosate on body malformations during embryonic development of zebrafish (Daino rerio)","volume":"180","author":"Sulukan","year":"2017","journal-title":"Chemosphere"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chemosphere.2011.08.033","article-title":"Effects of atrazine on the gill cells and ionic balance in a neotropical fish, Prochilodus lineatus","volume":"86","author":"Paulino","year":"2012","journal-title":"Chemosphere"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.resp.2006.03.007","article-title":"The structure and function of carbonic anhydrase isozymes in the respiratory system of vertebrates","volume":"154","author":"Esbaugh","year":"2006","journal-title":"Respir. Physiol. Neurobiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Mboge, M.Y., Mahon, B.P., McKenna, R., and Frost, S.C. (2018). Carbonic anhydrases: Role in pH control and cancer. Metabolites, 8.","DOI":"10.3390\/metabo8010019"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"5299","DOI":"10.1113\/JP271378","article-title":"Essential role of carbonic anhydrase XII in secretory gland fluid and HCO3\u2212 secretion revealed by diseasecausing human mutation","volume":"593","author":"Hong","year":"2015","journal-title":"J. Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Supuran, C.T. (2018). Carbonic anhydrases and metabolism. Metabolites, 8.","DOI":"10.3390\/metabo8020025"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1002\/mc.20448","article-title":"Carbonic anhydrase III promotes transformation and invasion capability in hepatoma cells through FAK signaling pathway","volume":"47","author":"Dai","year":"2008","journal-title":"Mol. Carcinog."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1080\/14756366.2016.1225046","article-title":"Insights into the role of reactive sulfhydryl groups of Carbonic Anhydrase III and VII during oxidative damage","volume":"32","author":"Monti","year":"2017","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"319","DOI":"10.3389\/fphys.2018.00319","article-title":"Functional involvement of carbonic anhydrase in the lysosomal response to cadmium exposure in Mytilus galloprovincialis digestive gland","volume":"9","author":"Caricato","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.scitotenv.2019.06.446","article-title":"Carbonic anhydrase integrated into a multimarker approach for the detection of the stress status induced by pollution exposure in Mytilus galloprovincialis: A field case study","volume":"690","author":"Caricato","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"231","DOI":"10.3109\/14756366.2012.700640","article-title":"Carbonic anhydrase III: A neglected isozyme is stepping into the limelight","volume":"28","author":"Harju","year":"2013","journal-title":"J. Enzyme Inhib. Med. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0009-8981(01)00682-9","article-title":"Alternations in quantities and activities of erythrocyte cytosolic carbonic anhydrase isoenzymes in glucose-6-phosphate dehydrogenase-deficient individuals","volume":"314","author":"Chiang","year":"2001","journal-title":"Clin. Chim. Acta"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.cccn.2004.07.009","article-title":"Differential expression of carbonic anhydrase isoenzymes invarious types of anemia","volume":"351","author":"Kuo","year":"2005","journal-title":"Clin. Chim. Acta"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4170","DOI":"10.1073\/pnas.93.9.4170","article-title":"The phosphatase activity of carbonic anhydrase III is reversibly regulated by glutathiolation","volume":"93","author":"Cabiscol","year":"1996","journal-title":"PNAS"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0014-5793(00)02022-6","article-title":"Crystal structure of S-glutathiolated carbonic anhydrase III","volume":"482","author":"Mallis","year":"2011","journal-title":"FEBS Lett."},{"key":"ref_61","first-page":"215","article-title":"Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout","volume":"152","author":"Ceyhun","year":"2010","journal-title":"Comp. Biochem. Physiol. C"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"106186","DOI":"10.1016\/j.ecolind.2020.106186","article-title":"Fish inhabiting rice fields: Bioaccumulation, oxidative stress and neurotoxic effects after pesticides application","volume":"113","author":"Rossi","year":"2020","journal-title":"Ecol. Indic."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0378-4274(99)00029-6","article-title":"Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers","volume":"107","author":"Banerjee","year":"1999","journal-title":"Toxicol. Lett."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1191\/0960327102ht238oa","article-title":"Induction of oxidative stress and acetylcholinesterase inhibition in organophosphorus pesticide manufacturing workers","volume":"21","author":"Ranjbar","year":"2002","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"503138","DOI":"10.1016\/j.mrgentox.2020.503138","article-title":"Oxidative stress-mediated genotoxicity of malathion in human lymphocytes","volume":"849","author":"Olakkaran","year":"2020","journal-title":"Mutat Res-Gen. Tox. En."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Carlsson, H., Rappaport, S.M., and T\u00f6rnqvist, M. (2019). Protein adductomics: Methodologies for untargeted screening of adducts to serum albumin and hemoglobin in human blood samples. High Throughput., 8.","DOI":"10.3390\/ht8010006"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.tox.2012.12.007","article-title":"Protein adducts as biomarkers of exposure to organophosphorus compounds","volume":"307","author":"Marsillach","year":"2013","journal-title":"Toxicology"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/10\/3562\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:29:58Z","timestamp":1760174998000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/10\/3562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,18]]},"references-count":67,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["ijms21103562"],"URL":"https:\/\/doi.org\/10.3390\/ijms21103562","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,18]]}}}