{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:51:50Z","timestamp":1790077910346,"version":"4.0.1"},"reference-count":33,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T00:00:00Z","timestamp":1590364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","award":["LP2016-8\/2016"],"award-info":[{"award-number":["LP2016-8\/2016"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","award":["460050"],"award-info":[{"award-number":["460050"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","award":["K131796"],"award-info":[{"award-number":["K131796"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005881","name":"Emberi Eroforr\u00e1sok Miniszt\u00e9riuma","doi-asserted-by":"publisher","award":["TUDFO\/4738-1\/2019 ITM"],"award-info":[{"award-number":["TUDFO\/4738-1\/2019 ITM"]}],"id":[{"id":"10.13039\/501100005881","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Lichtheimia corymbifera is considered as one of the most frequent agents of mucormycosis. The lack of efficient genetic manipulation tools hampers the characterization of the pathomechanisms and virulence factors of this opportunistic pathogenic fungus. Although such techniques have been described for certain species, the performance of targeted mutagenesis and the construction of stable transformants have remained a great challenge in Mucorales fungi. In the present study, a plasmid-free CRISPR-Cas9 system was applied to carry out a targeted gene disruption in L. corymbifera. The described method is based on the non-homologous end-joining repair of the double-strand break caused by the Cas9 enzyme. Using this method, short, one-to-five nucleotide long-targeted deletions could be induced in the orotidine 5\u2032-phosphate decarboxylase gene (pyrG) and, as a result, uracil auxotrophic strains were constructed. These strains are applicable as recipient strains in future gene manipulation studies. As we know, this is the first genetic modification of this clinically relevant fungus.<\/jats:p>","DOI":"10.3390\/ijms21103727","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T11:42:02Z","timestamp":1590406922000},"page":"3727","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["CRISPR-Cas9-Based Mutagenesis of the Mucormycosis-Causing Fungus Lichtheimia corymbifera"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7113-7360","authenticated-orcid":false,"given":"Sandugash","family":"Ibragimova","sequence":"first","affiliation":[{"name":"MTA-SZTE Fungal Pathogenicity Mechanisms Research Group, Hungarian Academy of Sciences\u2014University of Szeged, 6726 Szeged, Hungary"},{"name":"Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csilla","family":"Szebenyi","sequence":"additional","affiliation":[{"name":"MTA-SZTE Fungal Pathogenicity Mechanisms Research Group, Hungarian Academy of Sciences\u2014University of Szeged, 6726 Szeged, Hungary"},{"name":"Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Sinka","sequence":"additional","affiliation":[{"name":"Department of Genetics, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham I.","family":"Alzyoud","sequence":"additional","affiliation":[{"name":"Department of Genetics, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nika","family":"Homa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0009-7773","authenticated-orcid":false,"given":"Csaba","family":"V\u00e1gv\u00f6lgyi","sequence":"additional","affiliation":[{"name":"MTA-SZTE Fungal Pathogenicity Mechanisms Research Group, Hungarian Academy of Sciences\u2014University of Szeged, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7871-6098","authenticated-orcid":false,"given":"G\u00e1bor","family":"Nagy","sequence":"additional","affiliation":[{"name":"MTA-SZTE Fungal Pathogenicity Mechanisms Research Group, Hungarian Academy of Sciences\u2014University of Szeged, 6726 Szeged, Hungary"},{"name":"Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8211-5431","authenticated-orcid":false,"given":"Tam\u00e1s","family":"Papp","sequence":"additional","affiliation":[{"name":"MTA-SZTE Fungal Pathogenicity Mechanisms Research Group, Hungarian Academy of Sciences\u2014University of Szeged, 6726 Szeged, Hungary"},{"name":"Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1177\/1060028016655425","article-title":"Breaking the mold: A review of mucormycosis and current pharmacological treatment options","volume":"50","author":"Riley","year":"2016","journal-title":"Ann. 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