{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T05:21:21Z","timestamp":1783660881530,"version":"3.55.0"},"reference-count":45,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T00:00:00Z","timestamp":1590451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","award":["K115990"],"award-info":[{"award-number":["K115990"]}],"id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union and the European Regional Development Funds","award":["GINOP 2.3.2-15-2016-00006; EFOP 3.6.2-16-2017-00006"],"award-info":[{"award-number":["GINOP 2.3.2-15-2016-00006; EFOP 3.6.2-16-2017-00006"]}]},{"DOI":"10.13039\/501100005881","name":"Emberi Eroforr\u00e1sok Miniszt\u00e9riuma","doi-asserted-by":"publisher","award":["20391 3\/2018\/FEKUSTRAT"],"award-info":[{"award-number":["20391 3\/2018\/FEKUSTRAT"]}],"id":[{"id":"10.13039\/501100005881","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","award":["BO\/00522\/19\/8"],"award-info":[{"award-number":["BO\/00522\/19\/8"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015498","name":"Ministry for Innovation and Technology","doi-asserted-by":"publisher","award":["\u00daNKP-19-3-SZTE-269, \u00daNKP-18-4-SZTE-63, \u00daNKP-19-4-SZTE-89, \u00daNKP-19-4-SZTE-8"],"award-info":[{"award-number":["\u00daNKP-19-3-SZTE-269, \u00daNKP-18-4-SZTE-63, \u00daNKP-19-4-SZTE-89, \u00daNKP-19-4-SZTE-8"]}],"id":[{"id":"10.13039\/501100015498","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ischemic preconditioning (IPre) reduces ischemia\/reperfusion (I\/R) injury in the heart. The non-coding microRNA miR-125b-1-3p has been demonstrated to play a role in the mechanism of IPre. Hypercholesterolemia is known to attenuate the cardioprotective effect of preconditioning; nevertheless, the exact underlying mechanisms are not clear. Here we investigated, whether hypercholesterolemia influences the induction of miR-125b-1-3p by IPre. Male Wistar rats were fed with a rodent chow supplemented with 2% cholesterol and 0.25% sodium-cholate hydrate for 8 weeks to induce high blood cholesterol levels. The hearts of normo- and hypercholesterolemic animals were then isolated and perfused according to Langendorff, and were subjected to 35 min global ischemia and 120 min reperfusion with or without IPre (3 \u00d7 5 min I\/R cycles applied before index ischemia). IPre significantly reduced infarct size in the hearts of normocholesterolemic rats; however, IPre was ineffective in the hearts of hypercholesterolemic animals. Similarly, miR-125b-1-3p was upregulated by IPre in hearts of normocholesterolemic rats, while in the hearts of hypercholesterolemic animals IPre failed to increase miR-125b-1-3p significantly. Phosphorylation of cardiac Akt, ERK, and STAT3 was not significantly different in any of the groups at the end of reperfusion. Based on these results we propose here that hypercholesterolemia attenuates the upregulation of miR-125b-1-3p by IPre, which seems to be associated with the loss of cardioprotection.<\/jats:p>","DOI":"10.3390\/ijms21113744","type":"journal-article","created":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T05:35:52Z","timestamp":1590557752000},"page":"3744","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Hypercholesterolemia Interferes with Induction of miR-125b-1-3p in Preconditioned Hearts"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0415-5192","authenticated-orcid":false,"given":"M\u00e1rton R.","family":"Szab\u00f3","sequence":"first","affiliation":[{"name":"Metabolic Diseases and Cell Signaling (MEDICS) Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, D\u00f3m t\u00e9r 9., H-6720 Szeged, Hungary"},{"name":"Interdisciplinary Centre of Excellence, University of Szeged, Dugonics t\u00e9r 13., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e1ta","family":"G\u00e1sp\u00e1r","sequence":"additional","affiliation":[{"name":"Metabolic Diseases and Cell Signaling (MEDICS) Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, D\u00f3m t\u00e9r 9., H-6720 Szeged, Hungary"},{"name":"Interdisciplinary Centre of Excellence, University of Szeged, Dugonics t\u00e9r 13., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rton","family":"Pipicz","sequence":"additional","affiliation":[{"name":"Metabolic Diseases and Cell Signaling (MEDICS) Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, D\u00f3m t\u00e9r 9., H-6720 Szeged, Hungary"},{"name":"Interdisciplinary Centre of Excellence, University of Szeged, Dugonics t\u00e9r 13., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00f3ra","family":"Zsindely","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p fasor 52., H-6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Di\u00f3szegi","sequence":"additional","affiliation":[{"name":"Metabolic Diseases and Cell Signaling (MEDICS) Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, D\u00f3m t\u00e9r 9., H-6720 Szeged, Hungary"},{"name":"Interdisciplinary Centre of Excellence, University of Szeged, Dugonics t\u00e9r 13., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5929-2146","authenticated-orcid":false,"given":"M\u00e1rta","family":"S\u00e1rk\u00f6zy","sequence":"additional","affiliation":[{"name":"Metabolic Diseases and Cell Signaling (MEDICS) Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, D\u00f3m t\u00e9r 9., H-6720 Szeged, Hungary"},{"name":"Interdisciplinary Centre of Excellence, University of Szeged, Dugonics t\u00e9r 13., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8411-626X","authenticated-orcid":false,"given":"L\u00e1szl\u00f3","family":"Bodai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p fasor 52., H-6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tam\u00e1s","family":"Csont","sequence":"additional","affiliation":[{"name":"Metabolic Diseases and Cell Signaling (MEDICS) Research Group, Department of Biochemistry, Faculty of Medicine, University of Szeged, D\u00f3m t\u00e9r 9., H-6720 Szeged, Hungary"},{"name":"Interdisciplinary Centre of Excellence, University of Szeged, Dugonics t\u00e9r 13., H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e005375","DOI":"10.1161\/CIRCOUTCOMES.118.005375","article-title":"Mortality From Ischemic Heart Disease","volume":"12","author":"Nowbar","year":"2019","journal-title":"Circ. 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