{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:18:19Z","timestamp":1790651899677,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,5,31]],"date-time":"2020-05-31T00:00:00Z","timestamp":1590883200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["17930555"],"award-info":[{"award-number":["17930555"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K06474"],"award-info":[{"award-number":["19K06474"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K1816419"],"award-info":[{"award-number":["17K1816419"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["K09721"],"award-info":[{"award-number":["K09721"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Semenogelin 1 (SEMG1), a main component of human seminal plasma, is a multi-functional protein involved in the regulation of sperm motility and fertility. SEMG1 is orthologous to mouse seminal vesicle secretion 2 (SVS2), required for sperm survival in the female reproductive tract after copulation; however, its in vivo function remains unclear. In this study, we addressed this issue by examining the effect of recombinant SEMG1 on intrauterine mouse sperm survival. SEMG1 caused a dose-dependent decrease in mouse sperm motility, similar to its effect on human sperm, but SVS2 had no effect on mouse sperm motility. Mouse epididymal sperm in the presence of 100 \u00b5M SEMG1, a concentration that does not affect mouse sperm motility, were injected into the mouse uterus (intrauterine insemination, IUI). IUI combined with SEMG1 significantly increased the survival rate of intrauterine mouse sperm. The effect of SEMG1 on intrauterine sperm survival was comparable with that of SVS2. For clinical applications, three potentially sperm-protecting polypeptides that are easy to handle were designed from SEMG1, but their individual use was unable to mimic the ability of SEMG1. Our results indicate that SEMG1 has potential clinical applications for effective IUI and thereby for safe, simple, and effective internal fertilization.<\/jats:p>","DOI":"10.3390\/ijms21113961","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T04:09:03Z","timestamp":1591070943000},"page":"3961","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Human Semenogelin 1 Promotes Sperm Survival in the Mouse Female Reproductive Tract"],"prefix":"10.3390","volume":"21","author":[{"given":"Daiki","family":"Sakaguchi","sequence":"first","affiliation":[{"name":"Laboratory of Regulatory Biology, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8339-6738","authenticated-orcid":false,"given":"Kenji","family":"Miyado","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruaki","family":"Iwamoto","sequence":"additional","affiliation":[{"name":"Division of Male Infertility, Center for Human Reproduction, Sanno Hospital, International University of Health and Welfare, Tokyo 107-0052, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Urology, Dokkyo Medical University Saitama Medical Center, Saitama 343-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9805-2818","authenticated-orcid":false,"given":"Kaoru","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Faculty of Biomedical Engineering, Toin University of Yokohama, Kanagawa 225-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4880-3406","authenticated-orcid":false,"given":"Woojin","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4927-868X","authenticated-orcid":false,"given":"Manabu","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Misaki Marine Biological Station, School of Science, the University of Tokyo, Kanagawa 238-0225, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsuko","family":"Kawano","sequence":"additional","affiliation":[{"name":"Laboratory of Regulatory Biology, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1038\/168697b0","article-title":"Fertilizing capacity of spermatozoa deposited into the fallopian tubes","volume":"168","author":"Chang","year":"1951","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/170326a0","article-title":"The \u2019Capacitation\u2019 of the Mammalian Sperm","volume":"170","author":"Austin","year":"1952","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1095\/biolreprod.103.026435","article-title":"Cholesterol efflux alters lipid raft stability and distribution during capacitation of boar spermatozoa","volume":"71","author":"Shadan","year":"2004","journal-title":"Biol. 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